GSP1_ENCCU
ID GSP1_ENCCU Reviewed; 214 AA.
AC Q8SS11;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=GTP-binding nuclear protein GSP1;
DE AltName: Full=GTPase Ran homolog;
GN Name=GSP1; OrderedLocusNames=ECU04_1560;
OS Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC Encephalitozoon.
OX NCBI_TaxID=284813;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GB-M1;
RX PubMed=11719806; DOI=10.1038/35106579;
RA Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA Vivares C.P.;
RT "Genome sequence and gene compaction of the eukaryote parasite
RT Encephalitozoon cuniculi.";
RL Nature 414:450-453(2001).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL
RP STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=16691553; DOI=10.1002/pmic.200500796;
RA Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT (microsporidia): a reference map for proteins expressed in late sporogonial
RT stages.";
RL Proteomics 6:3625-3635(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH GDP.
RG Seattle structural genomics center for infectious disease (SSGCID);
RT "Crystal structure of a nuclear GTP-binding protein from Encephalitozoon
RT cuniculi bound to GDP-MG2+.";
RL Submitted (FEB-2012) to the PDB data bank.
CC -!- FUNCTION: GTP-binding protein involved in nucleocytoplasmic transport.
CC Required for the import of protein into the nucleus and also for RNA
CC export (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Found in a nuclear export complex with RanGTP, exportin and
CC pre-miRNA (By similarity). {ECO:0000250|UniProtKB:P62825}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC {ECO:0000269|PubMed:16691553}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ran family.
CC {ECO:0000305}.
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DR EMBL; AL590444; CAD25345.1; -; Genomic_DNA.
DR RefSeq; NP_584841.1; NM_001041191.1.
DR PDB; 4DJT; X-ray; 1.80 A; A/B=1-214.
DR PDBsum; 4DJT; -.
DR AlphaFoldDB; Q8SS11; -.
DR SMR; Q8SS11; -.
DR STRING; 284813.Q8SS11; -.
DR GeneID; 858989; -.
DR KEGG; ecu:ECU04_1560; -.
DR VEuPathDB; MicrosporidiaDB:ECU04_1560; -.
DR HOGENOM; CLU_041217_10_6_1; -.
DR InParanoid; Q8SS11; -.
DR OMA; IRFNIWD; -.
DR OrthoDB; 1083175at2759; -.
DR Proteomes; UP000000819; Chromosome IV.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002041; Ran_GTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR PANTHER; PTHR24071; PTHR24071; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Nucleotide-binding; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..214
FT /note="GTP-binding nuclear protein GSP1"
FT /id="PRO_0000382910"
FT BINDING 17..22
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0007744|PDB:4DJT"
FT BINDING 66
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 121..124
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0007744|PDB:4DJT"
FT BINDING 151..153
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0007744|PDB:4DJT"
FT STRAND 7..13
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 20..24
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:4DJT"
FT TURN 38..41
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 42..51
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 56..64
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 75..78
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 82..89
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 93..97
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 99..110
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 116..121
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 133..139
FT /evidence="ECO:0007829|PDB:4DJT"
FT TURN 140..142
FT /evidence="ECO:0007829|PDB:4DJT"
FT STRAND 146..151
FT /evidence="ECO:0007829|PDB:4DJT"
FT TURN 152..155
FT /evidence="ECO:0007829|PDB:4DJT"
FT TURN 157..159
FT /evidence="ECO:0007829|PDB:4DJT"
FT HELIX 160..170
FT /evidence="ECO:0007829|PDB:4DJT"
FT TURN 201..204
FT /evidence="ECO:0007829|PDB:4DJT"
SQ SEQUENCE 214 AA; 24364 MW; 27FD8429479467C7 CRC64;
MERRELTYKI CLIGDGGVGK TTYINRVLDG RFEKNYNATV GAVNHPVTFL DDQGNVIKFN
VWDTAGQEKK AVLKDVYYIG ASGAIFFFDV TSRITCQNLA RWVKEFQAVV GNEAPIVVCA
NKIDIKNRQK ISKKLVMEVL KGKNYEYFEI SAKTAHNFGL PFLHLARIFT GRPDLIFVSN
VNLEPTEVNY DYHSPEESKY IDYMEQASKM APEE
