GSPA_BACSU
ID GSPA_BACSU Reviewed; 286 AA.
AC P25148;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=General stress protein A;
GN Name=gspA; Synonyms=ywaG; OrderedLocusNames=BSU38430; ORFNames=ipa-12d;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=1806041; DOI=10.3109/10425179109020780;
RA Glaser P., Kunst F., Debarbouille M., Vertes A., Danchin A., Dedonder R.;
RT "A gene encoding a tyrosine tRNA synthetase is located near sacS in
RT Bacillus subtilis.";
RL DNA Seq. 1:251-261(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7934828; DOI=10.1111/j.1365-2958.1993.tb01963.x;
RA Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F.,
RA Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E.,
RA Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A.;
RT "Bacillus subtilis genome project: cloning and sequencing of the 97 kb
RT region from 325 degrees to 333 degrees.";
RL Mol. Microbiol. 10:371-384(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP PROTEIN SEQUENCE OF 1-28, CHARACTERIZATION, AND INDUCTION.
RX PubMed=7768864; DOI=10.1128/jb.177.12.3540-3545.1995;
RA Antelmann H., Bernhardt J., Schmid R., Hecker M.;
RT "A gene at 333 degrees on the Bacillus subtilis chromosome encodes the
RT newly identified sigma B-dependent general stress protein GspA.";
RL J. Bacteriol. 177:3540-3545(1995).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 110-286.
RC STRAIN=QB1072;
RX PubMed=2116367; DOI=10.1016/0378-1119(90)90453-x;
RA Zukowski M.M., Miller L., Cosgwell P., Chen K., Aymerich S., Steinmetz M.;
RT "Nucleotide sequence of the sacS locus of Bacillus subtilis reveals the
RT presence of two regulatory genes.";
RL Gene 90:153-155(1990).
CC -!- INDUCTION: By different stresses such as heat shock and salt stress and
CC by starvation. {ECO:0000269|PubMed:7768864}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA36721.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X52480; CAA36721.1; ALT_INIT; Genomic_DNA.
DR EMBL; X73124; CAA51568.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15869.1; -; Genomic_DNA.
DR EMBL; M29333; AAA75337.1; -; Genomic_DNA.
DR PIR; S16423; S16423.
DR RefSeq; NP_391722.1; NC_000964.3.
DR RefSeq; WP_003242748.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P25148; -.
DR SMR; P25148; -.
DR STRING; 224308.BSU38430; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR PaxDb; P25148; -.
DR PRIDE; P25148; -.
DR DNASU; 937339; -.
DR EnsemblBacteria; CAB15869; CAB15869; BSU_38430.
DR GeneID; 937339; -.
DR KEGG; bsu:BSU38430; -.
DR PATRIC; fig|224308.179.peg.4160; -.
DR eggNOG; COG1442; Bacteria.
DR InParanoid; P25148; -.
DR OMA; YMNEICE; -.
DR PhylomeDB; P25148; -.
DR BioCyc; BSUB:BSU38430-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosyltransferase; Manganese; Metal-binding;
KW Nucleotide-binding; Reference proteome; Stress response; Transferase.
FT CHAIN 1..286
FT /note="General stress protein A"
FT /id="PRO_0000083867"
FT BINDING 12..17
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 111..112
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 111
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 113
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 247..253
FT /ligand="UDP"
FT /ligand_id="ChEBI:CHEBI:58223"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT BINDING 247
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:A0A0H2URJ6"
FT CONFLICT 12
FT /note="C -> A (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 27
FT /note="S -> V (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 286 AA; 33522 MW; A282BE6E3B6518DE CRC64;
MRKDEIMHIV SCADDNYARH LGGMFVSLLT NMDQEREVKL YVIDGGIKPD NKKRLEETTL
KFGVPIEFLE VDTNMYEHAV ESSHITKAAY YRISIPDLIK DESIKRMIYI DCDALVLEDI
SKLWDLDIAP YTVAAVEDAG QHERLKEMNV TDTGKYFNSG IMIIDFESWR KQNITEKVIN
FINEHPDEDF LVLHDQDALN AILYDQWYEL HPRWNAQTYI MLKLKTPSTL LGRKQYNETR
ENPAIVHFCG GEKPWNSNTK HPYRDEYFHY MSYTKWNTIG NPAINQ
