ID   GSPA_ECOLI              Reviewed;         489 AA.
AC   P45756; Q2M6Y8;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Putative general secretion pathway protein A;
GN   Name=gspA; Synonyms=yheD; OrderedLocusNames=b3323, JW3285;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   LACK OF EXPRESSION.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=8655552; DOI=10.1128/jb.178.12.3544-3549.1996;
RA   Francetic O., Pugsley A.P.;
RT   "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-
RT   12 encodes functional proteins.";
RL   J. Bacteriol. 178:3544-3549(1996).
RN   [4]
RP   LACK OF EXPRESSION, FUNCTION, AND TRANSCRIPTIONAL REGULATION.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=11118204; DOI=10.1093/emboj/19.24.6697;
RA   Francetic O., Belin D., Badaut C., Pugsley A.P.;
RT   "Expression of the endogenous type II secretion pathway in Escherichia coli
RT   leads to chitinase secretion.";
RL   EMBO J. 19:6697-6703(2000).
CC   -!- FUNCTION: May play a regulatory role under conditions of derepressed
CC       gsp gene expression. {ECO:0000269|PubMed:11118204}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Silenced by the DNA-binding protein H-NS under standard
CC       growth conditions. {ECO:0000269|PubMed:11118204}.
CC   -!- MISCELLANEOUS: Part of a cryptic operon that encodes proteins involved
CC       in type II secretion machinery in other organisms, but is not expressed
CC       in strain K12.
CC   -!- SIMILARITY: Belongs to the ExeA family. {ECO:0000305}.
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DR   EMBL; U18997; AAA58120.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76348.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77968.1; -; Genomic_DNA.
DR   PIR; F65125; F65125.
DR   RefSeq; NP_417782.1; NC_000913.3.
DR   RefSeq; WP_000107576.1; NZ_STEB01000038.1.
DR   AlphaFoldDB; P45756; -.
DR   SMR; P45756; -.
DR   BioGRID; 4261295; 259.
DR   IntAct; P45756; 1.
DR   STRING; 511145.b3323; -.
DR   TCDB; 9.B.42.1.3; the exeab (exeab) secretin assembly/export complex.
DR   PaxDb; P45756; -.
DR   PRIDE; P45756; -.
DR   EnsemblBacteria; AAC76348; AAC76348; b3323.
DR   EnsemblBacteria; BAE77968; BAE77968; BAE77968.
DR   GeneID; 947825; -.
DR   KEGG; ecj:JW3285; -.
DR   KEGG; eco:b3323; -.
DR   PATRIC; fig|1411691.4.peg.3408; -.
DR   EchoBASE; EB2725; -.
DR   eggNOG; COG3267; Bacteria.
DR   eggNOG; COG3409; Bacteria.
DR   HOGENOM; CLU_044050_0_0_6; -.
DR   InParanoid; P45756; -.
DR   OMA; WFTRHFS; -.
DR   BioCyc; EcoCyc:G7701-MON; -.
DR   PRO; PR:P45756; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.101.10; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002477; Peptidoglycan-bd-like.
DR   InterPro; IPR036365; PGBD-like_sf.
DR   InterPro; IPR036366; PGBDSf.
DR   Pfam; PF13401; AAA_22; 1.
DR   Pfam; PF01471; PG_binding_1; 1.
DR   SUPFAM; SSF47090; SSF47090; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..489
FT                   /note="Putative general secretion pathway protein A"
FT                   /id="PRO_0000214994"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         26..33
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   489 AA;  55298 MW;  7C7DDB238274E15C CRC64;
     MSTRREVILS WLCEKRQTWR LCYLLGEAGS GKTWLAQQLQ KDKHRRVITL SLVVSWQGKA
     AWIVTDDNAA EQGCRDSAWT RDEMAGQLLH ALHRTDSRCP LIIIENAHLN HRRILDDLQR
     AISLIPDGQF LLIGRPDRKV ERDFKKQGIE LVSIGRLTEH ELKASILEGQ NIDQPDLLLT
     ARVLKRIALL CRGDRRKLAL AGETIRLLQQ AEQTSVFTAK QWRMIYRILG DNRPRKMQLA
     VVMSGTIIAL TCGWLLLSSF TATLPVPAWL IPVTPVVKQD MTKDIAHVVM RDSEALSVLY
     GVWGYEVPAD SAWCDQAVRA GLACKSGNAS LQTLVDQNLP WIASLKVGDK KLPVVVVRVG
     EASVDVLVGQ QTWTLTHKWF ESVWTGDYLL LWKMSPEGES TITRDSSEEE ILWLETMLNR
     ALHISTEPSA EWRPLLVEKI KQFQKSHHLK TDGVVGFSTL VHLWQVAGES AYLYRDEANI
     SPETTVKGK