ID   GSPB_DICD3              Reviewed;         220 AA.
AC   Q01563; E0SM44;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=General secretion pathway protein B;
GN   Name=outB; OrderedLocusNames=Dda3937_02412;
OS   Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=198628;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=3937;
RX   PubMed=1453958; DOI=10.1111/j.1365-2958.1992.tb01775.x;
RA   Condemine G., Dorel C., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J.;
RT   "Some of the out genes involved in the secretion of pectate lyases in
RT   Erwinia chrysanthemi are regulated by kdgR.";
RL   Mol. Microbiol. 6:3199-3211(1992).
RN   [2]
RP   SEQUENCE REVISION.
RA   Condemine G.;
RL   Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3937;
RX   PubMed=21217001; DOI=10.1128/jb.01513-10;
RA   Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA   Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA   Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA   Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA   Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA   Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA   Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA   Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA   Blattner F.R., Keen N.T., Perna N.T.;
RT   "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL   J. Bacteriol. 193:2076-2077(2011).
CC   -!- FUNCTION: Out proteins are required for the translocation of pectate
CC       lyases and cellulases across the outer membrane.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ExeB/OutB/PulB family. {ECO:0000305}.
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DR   EMBL; X65265; CAA46373.1; -; Genomic_DNA.
DR   EMBL; CP002038; ADM99379.1; -; Genomic_DNA.
DR   RefSeq; WP_013318813.1; NC_014500.1.
DR   PDB; 4WFW; X-ray; 2.05 A; A=112-201.
DR   PDBsum; 4WFW; -.
DR   AlphaFoldDB; Q01563; -.
DR   SMR; Q01563; -.
DR   STRING; 198628.Dda3937_02412; -.
DR   EnsemblBacteria; ADM99379; ADM99379; Dda3937_02412.
DR   GeneID; 9734628; -.
DR   KEGG; ddd:Dda3937_02412; -.
DR   PATRIC; fig|198628.6.peg.3140; -.
DR   eggNOG; COG3266; Bacteria.
DR   HOGENOM; CLU_1426772_0_0_6; -.
DR   OMA; DSLQDWP; -.
DR   OrthoDB; 1461076at2; -.
DR   BioCyc; DDAD198628:DDA3937_RS14815-MON; -.
DR   Proteomes; UP000006859; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0016485; P:protein processing; IMP:ASAP.
DR   InterPro; IPR032389; GspB.
DR   Pfam; PF16537; T2SSB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..220
FT                   /note="General secretion pathway protein B"
FT                   /id="PRO_0000214996"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          85..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          188..220
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        85..110
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          132..136
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   HELIX           141..143
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   STRAND          145..148
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   STRAND          151..153
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   STRAND          163..168
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   STRAND          170..177
FT                   /evidence="ECO:0007829|PDB:4WFW"
FT   STRAND          180..185
FT                   /evidence="ECO:0007829|PDB:4WFW"
SQ   SEQUENCE   220 AA;  23486 MW;  D094FE2DC0931978 CRC64;
     MKNTPEVKAS PQTGYRIPGY LLVVYALLLF TLGWFGHQRW ADISPIPLST SAAAIAAPPT
     KVGMVPASTA VTADENHPGS LHAAAENSAT QTAASGSQTS ASSQEATPDT KPAKLVTGWQ
     TAKPGELPYI AFSAHVYTSA PDKRSVTLNG ERYREGDSPY QGLVIEQIEQ DMVIFSFNGE
     PFILDSLQDW PGGKPGDDAA QGNEQEPTSK PEQTVRTTKK