ID   GSPC_ECOLI              Reviewed;         271 AA.
AC   P45757; Q2M6Y9;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=Putative type II secretion system protein C;
DE            Short=T2SS protein C;
DE   AltName: Full=Putative general secretion pathway protein C;
GN   Name=gspC; Synonyms=yheE; OrderedLocusNames=b3324, JW3286;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   LACK OF EXPRESSION.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=8655552; DOI=10.1128/jb.178.12.3544-3549.1996;
RA   Francetic O., Pugsley A.P.;
RT   "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-
RT   12 encodes functional proteins.";
RL   J. Bacteriol. 178:3544-3549(1996).
RN   [4]
RP   LACK OF EXPRESSION, AND TRANSCRIPTIONAL REGULATION.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=11118204; DOI=10.1093/emboj/19.24.6697;
RA   Francetic O., Belin D., Badaut C., Pugsley A.P.;
RT   "Expression of the endogenous type II secretion pathway in Escherichia coli
RT   leads to chitinase secretion.";
RL   EMBO J. 19:6697-6703(2000).
CC   -!- FUNCTION: Involved in a type II secretion system (T2SS, formerly
CC       general secretion pathway, GSP) for the export of proteins.
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Silenced by the DNA-binding protein H-NS under standard
CC       growth conditions. {ECO:0000269|PubMed:11118204}.
CC   -!- MISCELLANEOUS: Part of a cryptic operon that encodes proteins involved
CC       in type II secretion machinery in other organisms, but is not expressed
CC       in strain K12.
CC   -!- SIMILARITY: Belongs to the GSP C family. {ECO:0000305}.
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DR   EMBL; U18997; AAA58121.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76349.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77967.1; -; Genomic_DNA.
DR   PIR; G65125; G65125.
DR   RefSeq; NP_417783.1; NC_000913.3.
DR   RefSeq; WP_001142708.1; NZ_SSZK01000040.1.
DR   AlphaFoldDB; P45757; -.
DR   SMR; P45757; -.
DR   BioGRID; 4261296; 198.
DR   STRING; 511145.b3324; -.
DR   PaxDb; P45757; -.
DR   PRIDE; P45757; -.
DR   EnsemblBacteria; AAC76349; AAC76349; b3324.
DR   EnsemblBacteria; BAE77967; BAE77967; BAE77967.
DR   GeneID; 947824; -.
DR   KEGG; ecj:JW3286; -.
DR   KEGG; eco:b3324; -.
DR   PATRIC; fig|1411691.4.peg.3407; -.
DR   EchoBASE; EB2726; -.
DR   eggNOG; COG3031; Bacteria.
DR   HOGENOM; CLU_102113_0_0_6; -.
DR   OMA; ICCSTIA; -.
DR   BioCyc; EcoCyc:G7702-MON; -.
DR   BioCyc; MetaCyc:G7702-MON; -.
DR   PRO; PR:P45757; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProt.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProt.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR024961; T2SS_GspC_N.
DR   InterPro; IPR001639; T2SS_protein-GspC.
DR   Pfam; PF11356; T2SSC; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR01713; typeII_sec_gspC; 1.
DR   PROSITE; PS01141; T2SP_C; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..271
FT                   /note="Putative type II secretion system protein C"
FT                   /id="PRO_0000215000"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        39..271
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   271 AA;  30038 MW;  439E9EDCA50C5E28 CRC64;
     MPTLRFPFHL ANHNKDAAIN ILIIFISIGS IIFNVNYFHT TIVKNGQIIN QPTNAFQSDF
     SLAALWRNEN HAGVKDANPV AVNQETPKLS IALNGIVLTS NDETSFVLIN EGSEQKRYSL
     NEALESAPGT FIRKINKTSV VFETHGHYEK VTLHPGLPDI IKQPDSESQN VLADYIIATP
     IRDGEQIYGL RLNPRKGLNA FTTSLLQPGD IALRINNLSL THPDEVSQAL SLLLTQQSAQ
     FTIRRNGVPR LINVSVGELT GMNGLRHERT Q