GSPF_KLEPN
ID GSPF_KLEPN Reviewed; 381 AA.
AC P15745;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Type II secretion system protein F;
DE Short=T2SS protein F;
DE AltName: Full=General secretion pathway protein F;
DE AltName: Full=Pullulanase secretion protein PulF;
GN Name=pulF;
OS Klebsiella pneumoniae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Pugsley A.P., D'Enfert C., Reyss I., Wandersman C.;
RL Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION.
RX PubMed=1738317; DOI=10.1111/j.1365-2958.1992.tb00841.x;
RA Possot O., d'Enfert C., Reyss I., Pugsley A.P.;
RT "Pullulanase secretion in Escherichia coli K-12 requires a cytoplasmic
RT protein and a putative polytopic cytoplasmic membrane protein.";
RL Mol. Microbiol. 6:95-105(1992).
CC -!- FUNCTION: Component of the type II secretion system inner membrane
CC complex required for the energy-dependent secretion of extracellular
CC factors such as proteases and toxins from the periplasm.
CC {ECO:0000250|UniProtKB:Q00514}.
CC -!- SUBUNIT: Type II secretion system is composed of four main components:
CC the outer membrane complex, the inner membrane complex, the cytoplasmic
CC secretion ATPase and the periplasm-spanning pseudopilus (By
CC similarity). Homodimer (By similarity). Interacts with PulE and PulL
CC components (By similarity). {ECO:0000250|UniProtKB:P45780,
CC ECO:0000250|UniProtKB:Q00513, ECO:0000250|UniProtKB:Q00514}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GSP F family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M32613; AAA25128.1; -; Genomic_DNA.
DR PIR; S20035; S20035.
DR AlphaFoldDB; P15745; -.
DR SMR; P15745; -.
DR TCDB; 3.A.15.1.1; the outer membrane protein secreting main terminal branch (mtb) family.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 1.20.81.30; -; 2.
DR InterPro; IPR003004; GspF/PilC.
DR InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR InterPro; IPR018076; T2SS_GspF_dom.
DR InterPro; IPR042094; T2SS_GspF_sf.
DR PANTHER; PTHR30012; PTHR30012; 2.
DR Pfam; PF00482; T2SSF; 2.
DR PRINTS; PR00812; BCTERIALGSPF.
DR PROSITE; PS00874; T2SP_F; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..381
FT /note="Type II secretion system protein F"
FT /id="PRO_0000207834"
FT TOPO_DOM 1..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..197
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..381
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P41441"
SQ SEQUENCE 381 AA; 41873 MW; 01347C0994B29933 CRC64;
MALFRYQALD EQGKPRRGVQ QADSARHARQ LLREKGWLAL DIDPAAGGGR PSRFMRRTSA
RDLALVTRQL ATLVAAAIPL EKALDAVAQQ SEKPQLKTLI AGVRGKVLEG HSLAEAMRGH
PGCFDALYCA MVAAGEASGH RLLQAMIYPI VLTLVAVSVI VILLSTVVPK VVEQFIHLKQ
ALPFSTRLLM AMSDMLRAAG PWLLLAILLL ILLLRYLLRQ PAKRLAWHRL LLRLPLIGRV
ARSVNSARYA RTLSILNASA VPLLLAMRIS ADVLSNAWAK RQLEAASDAV REGVSLHRAL
EMTQLFPPMM RYMVASGERS GELNSMLERA ADNQDRDLSA QIQLALSLFE PLLVVAMAGM
VLFIVLAILQ PILQLNTLMS M