GSPF_XANCP
ID GSPF_XANCP Reviewed; 405 AA.
AC P31744;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 3.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Type II secretion system protein F;
DE Short=T2SS protein F;
DE AltName: Full=General secretion pathway protein F;
GN Name=xpsF; Synonyms=pefF; OrderedLocusNames=XCC0661;
OS Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB
OS 528 / LMG 568 / P 25).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=190485;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=8000 NCPPB 1145;
RX PubMed=1944223; DOI=10.1007/bf00267456;
RA Dums F., Dow J.M., Daniels M.J.;
RT "Structural characterization of protein secretion genes of the bacterial
RT phytopathogen Xanthomonas campestris pathovar campestris: relatedness to
RT secretion systems of other Gram-negative bacteria.";
RL Mol. Gen. Genet. 229:357-364(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Xc1701;
RA Hu N.-T.T., Hung M.-N., Wang K.C.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25;
RX PubMed=12024217; DOI=10.1038/417459a;
RA da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT specificities.";
RL Nature 417:459-463(2002).
CC -!- FUNCTION: Component of the type II secretion system inner membrane
CC complex required for the energy-dependent secretion of extracellular
CC factors such as proteases and toxins from the periplasm.
CC {ECO:0000250|UniProtKB:Q00514}.
CC -!- SUBUNIT: Type II secretion system is composed of four main components:
CC the outer membrane complex, the inner membrane complex, the cytoplasmic
CC secretion ATPase and the periplasm-spanning pseudopilus (By
CC similarity). Homodimer (By similarity). Interacts with XpsE and XpsL
CC components (By similarity). {ECO:0000250|UniProtKB:P45780,
CC ECO:0000250|UniProtKB:Q00513, ECO:0000250|UniProtKB:Q00514}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GSP F family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA41804.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X59079; CAA41804.1; ALT_INIT; Genomic_DNA.
DR EMBL; L02630; AAC27376.3; -; Genomic_DNA.
DR EMBL; AE008922; AAM39977.1; -; Genomic_DNA.
DR PIR; S17938; S17938.
DR PIR; T12057; T12057.
DR RefSeq; NP_636053.1; NC_003902.1.
DR RefSeq; WP_011035901.1; NC_003902.1.
DR AlphaFoldDB; P31744; -.
DR SMR; P31744; -.
DR STRING; 340.xcc-b100_3694; -.
DR EnsemblBacteria; AAM39977; AAM39977; XCC0661.
DR KEGG; xcc:XCC0661; -.
DR PATRIC; fig|190485.4.peg.725; -.
DR eggNOG; COG1459; Bacteria.
DR HOGENOM; CLU_035032_2_1_6; -.
DR OMA; YVVPQFV; -.
DR Proteomes; UP000001010; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IBA:GO_Central.
DR Gene3D; 1.20.81.30; -; 2.
DR InterPro; IPR003004; GspF/PilC.
DR InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR InterPro; IPR018076; T2SS_GspF_dom.
DR InterPro; IPR042094; T2SS_GspF_sf.
DR PANTHER; PTHR30012; PTHR30012; 1.
DR Pfam; PF00482; T2SSF; 2.
DR PRINTS; PR00812; BCTERIALGSPF.
DR PROSITE; PS00874; T2SP_F; 1.
PE 3: Inferred from homology;
KW Calcium; Cell inner membrane; Cell membrane; Membrane; Metal-binding;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..405
FT /note="Type II secretion system protein F"
FT /id="PRO_0000207837"
FT TOPO_DOM 1..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..218
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q00513"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..405
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P41441"
FT BINDING 98
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P45780"
FT BINDING 151
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P45780"
FT BINDING 155
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P45780"
FT CONFLICT 67
FT /note="A -> S (in Ref. 2; AAC27376)"
FT /evidence="ECO:0000305"
FT CONFLICT 114..115
FT /note="GG -> A (in Ref. 1; CAA41804)"
FT /evidence="ECO:0000305"
FT CONFLICT 346
FT /note="G -> A (in Ref. 1; CAA41804)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 405 AA; 44024 MW; B5623E53CD137E23 CRC64;
MPLYRYKALD AHGEMLDGQM EAANDAEVAL RLQEQGHLPV ETRLATGENG SPSLRMLLRK
KPFDNAALVQ FTQQLATLIG AGQPLDRALS ILMDLPEDDK SRRVIADIRD TVRGGAPLSV
ALERQHGLFS KLYINMVRAG EAGGSMQDTL QRLADYLERS RALKGKVINA LIYPAILLAV
VGCALLFLLG YVVPQFAQMY ESLDVALPWF TQAVLSVGLL VRDWWLVLVV IPGVLGLWLD
RKRRNAAFRA ALDAWLLRQK VIGSLIARLE TARLTRTLGT LLRNGVPLLA AIGIARNVMS
NTALVEDVAA AADDVKNGHG LSMSLARGKR FPRLALQMIQ VGEESGALDT MLLKTADTFE
LETAQAIDRA LAALVPLITL VLASVVGLVI ISVLVPLYDL TNAIG
