ID   GSPL_DICCH              Reviewed;         399 AA.
AC   P31707;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Type II secretion system protein L;
DE            Short=T2SS protein L;
DE   AltName: Full=General secretion pathway protein L;
DE   AltName: Full=Pectic enzymes secretion protein OutL;
GN   Name=outL;
OS   Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=556;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EC16;
RX   PubMed=1429461; DOI=10.1128/jb.174.22.7385-7397.1992;
RA   Lindeberg M., Collmer A.;
RT   "Analysis of eight out genes in a cluster required for pectic enzyme
RT   secretion by Erwinia chrysanthemi: sequence comparison with secretion genes
RT   from other Gram-negative bacteria.";
RL   J. Bacteriol. 174:7385-7397(1992).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH OUTE.
RX   PubMed=10356336; DOI=10.1006/jmbi.1999.2803;
RA   Py B., Loiseau L., Barras F.;
RT   "Assembly of the type II secretion machinery of Erwinia chrysanthemi:
RT   direct interaction and associated conformational change between OutE, the
RT   putative ATP-binding component and the membrane protein OutL.";
RL   J. Mol. Biol. 289:659-670(1999).
RN   [3]
RP   INTERACTION WITH OUTE/GSPE; OUTE/GSPE AND OUTM/GSPM, AND FUNCTION.
RX   PubMed=11266368; DOI=10.1093/embo-reports/kve042;
RA   Py B., Loiseau L., Barras F.;
RT   "An inner membrane platform in the type II secretion machinery of Gram-
RT   negative bacteria.";
RL   EMBO Rep. 2:244-248(2001).
CC   -!- FUNCTION: Inner membrane component of the type II secretion system
CC       required for the energy-dependent secretion of extracellular factors
CC       such as proteases and toxins from the periplasm. Plays a role in the
CC       complex assembly and recruits OutM resulting in a stable complex in the
CC       inner membrane (By similarity). Provides thus a link between the
CC       energy-providing OutE protein in the cytoplasm and the rest of the T2SS
CC       machinery (PubMed:10356336, PubMed:11266368).
CC       {ECO:0000250|UniProtKB:P25060, ECO:0000269|PubMed:10356336,
CC       ECO:0000269|PubMed:11266368}.
CC   -!- SUBUNIT: Type II secretion system is composed of four main components:
CC       the outer membrane complex, the inner membrane complex, the cytoplasmic
CC       secretion ATPase and the periplasm-spanning pseudopilus (By
CC       similarity). Forms homodimers. Interacts with OutM/GspM
CC       (PubMed:11266368). Interacts with OutE/GspE and OutF/GspF
CC       (PubMed:10356336, PubMed:11266368). {ECO:0000250|UniProtKB:Q00514,
CC       ECO:0000269|PubMed:10356336, ECO:0000269|PubMed:11266368}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P25060}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P25060}.
CC   -!- SIMILARITY: Belongs to the GSP L family. {ECO:0000305}.
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DR   EMBL; L02214; AAA24839.1; -; Genomic_DNA.
DR   PIR; A47755; A47755.
DR   AlphaFoldDB; P31707; -.
DR   SMR; P31707; -.
DR   GO; GO:0009276; C:Gram-negative-bacterium-type cell wall; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR024230; GspL_cyto_dom.
DR   InterPro; IPR025691; GspL_pp_dom.
DR   InterPro; IPR007812; T2SS_protein-GspL.
DR   Pfam; PF12693; GspL_C; 1.
DR   Pfam; PF05134; T2SSL; 1.
DR   PIRSF; PIRSF015761; Protein_L; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR01709; typeII_sec_gspL; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..399
FT                   /note="Type II secretion system protein L"
FT                   /id="PRO_0000207316"
FT   TOPO_DOM        1..247
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25060"
FT   TRANSMEM        248..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        265..399
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25060"
SQ   SEQUENCE   399 AA;  45405 MW;  154138F5E39394BD CRC64;
     MSKAENTSGK QQLILRLSAD TSDSLEWLIW SVSRHQTLTT GSGTLESLQA VLADYPVISA
     RVLVPSTDVT FHTLSLPRQS RRQLLQAIPF MLEEQVASDI DQLHFAVMDM HGDNATVAVV
     QKSRLRAWLN QCETLGVPVE TVVPDVMALP RADSAWSAIS HRNLWLFRLD SGIGMAAEEN
     WYQSLLAFQP LPAVHCYSPV PASALTWQPQ PVTDLLTLAA QVNLSMSMDL RQGEYAPVKP
     WKQALLPWRN VLIALSAWLL LVLGESVWTH YQWYRQADYW RQESVRVYRK LFPDEKQVVN
     PRAQMQRHLQ EVRAGVSGFA LTEQMNRLQQ LVAQNEGVSL QSLSYDRSRD ELRLSLRATS
     YAQMEQFRQQ AQAYFQIPPG EMKQEKDHVE GQLTLRSQP