GSPL_ECOLI
ID GSPL_ECOLI Reviewed; 387 AA.
AC P45763; Q2M6Z8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Putative type II secretion system protein L;
DE Short=T2SS protein L;
DE AltName: Full=Putative general secretion pathway protein L;
GN Name=gspL; Synonyms=yheK; OrderedLocusNames=b3333, JW5705;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP LACK OF EXPRESSION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=8655552; DOI=10.1128/jb.178.12.3544-3549.1996;
RA Francetic O., Pugsley A.P.;
RT "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-
RT 12 encodes functional proteins.";
RL J. Bacteriol. 178:3544-3549(1996).
RN [4]
RP LACK OF EXPRESSION, AND TRANSCRIPTIONAL REGULATION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=11118204; DOI=10.1093/emboj/19.24.6697;
RA Francetic O., Belin D., Badaut C., Pugsley A.P.;
RT "Expression of the endogenous type II secretion pathway in Escherichia coli
RT leads to chitinase secretion.";
RL EMBO J. 19:6697-6703(2000).
CC -!- FUNCTION: Inner membrane component of the type II secretion system
CC required for the energy-dependent secretion of extracellular factors
CC such as proteases and toxins from the periplasm. Plays a role in the
CC complex assembly and recruits GspM resulting in a stable complex in the
CC inner membrane. Provides thus a link between the energy-providing GspE
CC protein in the cytoplasm and the rest of the T2SS machinery.
CC {ECO:0000250|UniProtKB:P25060}.
CC -!- SUBUNIT: Type II secretion system is composed of four main components:
CC the outer membrane complex, the inner membrane complex, the cytoplasmic
CC secretion ATPase and the periplasm-spanning pseudopilus (By
CC similarity). Forms homodimers. Interacts with GspM. Interacts with GspE
CC and GspF (By similarity). {ECO:0000250|UniProtKB:P25060,
CC ECO:0000250|UniProtKB:Q00514}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P25060}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P25060}.
CC -!- INDUCTION: Silenced by the DNA-binding protein H-NS under standard
CC growth conditions. {ECO:0000269|PubMed:11118204}.
CC -!- MISCELLANEOUS: Part of a cryptic operon that encodes proteins involved
CC in type II secretion machinery in other organisms, but is not expressed
CC in strain K12.
CC -!- SIMILARITY: Belongs to the GSP L family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA58130.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U18997; AAA58130.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76358.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77958.1; -; Genomic_DNA.
DR PIR; H65126; H65126.
DR RefSeq; NP_417792.2; NC_000913.3.
DR RefSeq; WP_001115238.1; NZ_SSZK01000040.1.
DR AlphaFoldDB; P45763; -.
DR SMR; P45763; -.
DR BioGRID; 4263437; 79.
DR STRING; 511145.b3333; -.
DR PaxDb; P45763; -.
DR PRIDE; P45763; -.
DR EnsemblBacteria; AAC76358; AAC76358; b3333.
DR EnsemblBacteria; BAE77958; BAE77958; BAE77958.
DR GeneID; 947842; -.
DR KEGG; ecj:JW5705; -.
DR KEGG; eco:b3333; -.
DR PATRIC; fig|1411691.4.peg.3398; -.
DR EchoBASE; EB2732; -.
DR eggNOG; COG3297; Bacteria.
DR HOGENOM; CLU_061518_0_0_6; -.
DR OMA; HFAILHQ; -.
DR BioCyc; EcoCyc:G7711-MON; -.
DR BioCyc; MetaCyc:G7711-MON; -.
DR PRO; PR:P45763; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009276; C:Gram-negative-bacterium-type cell wall; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR024230; GspL_cyto_dom.
DR InterPro; IPR025691; GspL_pp_dom.
DR InterPro; IPR007812; T2SS_protein-GspL.
DR Pfam; PF12693; GspL_C; 1.
DR Pfam; PF05134; T2SSL; 1.
DR PIRSF; PIRSF015761; Protein_L; 1.
DR SUPFAM; SSF53067; SSF53067; 1.
DR TIGRFAMs; TIGR01709; typeII_sec_gspL; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..387
FT /note="Putative type II secretion system protein L"
FT /id="PRO_0000207314"
FT TOPO_DOM 1..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P25060"
FT TRANSMEM 242..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 261..387
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P25060"
SQ SEQUENCE 387 AA; 44540 MW; 3F123EEF75B9295A CRC64;
MPESLMVIRS SSTLRKHWEW MTFSADSVSS VHTLTDDLPL ESLADQPGAG NVHLLIPPEG
LLYRSLTLPN AKYKLTAQTL QWLAEETLPD NTQDWHWTVV DKQNESVEVI GIQSEKLSRY
LERLHTAGLN VTRVLPDGCY LPWEVDSWTL VNQQTSWLIR SAAHAFNELD EHWLQHLAAQ
FPPENMLCYG VVPHGVAAAN PLIQHPEIPS LSLYSADIAF QRYDMLHGIF RKQKTVSKSG
KWLARLAVSC LVLAILSFVG SRSIALWHTL KIEDQLQQQQ QETWQRYFPQ IKRTHNFRFY
FKQQLAQQYP EAVPLLYHLQ TLLLEHPELQ LMEANYSQKQ KSLTLKMSAK SEANIDRFCE
LTQSWLPMEK TEKDPVSGVW TVRNSGK
