ID   GSPL_KLEPN              Reviewed;         398 AA.
AC   P15751;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Type II secretion system protein L;
DE            Short=T2SS protein L;
DE   AltName: Full=General secretion pathway protein L;
DE   AltName: Full=Pullulanase secretion protein PulL;
GN   Name=pulL;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UNF 5023;
RX   PubMed=2162463; DOI=10.1111/j.1365-2958.1990.tb00604.x;
RA   Pugsley A.P., Reyss I.;
RT   "Five genes at the 3' end of the Klebsiella pneumoniae pulC operon are
RT   required for pullulanase secretion.";
RL   Mol. Microbiol. 4:365-379(1990).
CC   -!- FUNCTION: Inner membrane component of the type II secretion system
CC       required for the energy-dependent secretion of extracellular factors
CC       such as proteases and toxins from the periplasm. Plays a role in the
CC       complex assembly and recruits PulM resulting in a stable complex in the
CC       inner membrane. Provides thus a link between the energy-providing PulE
CC       protein in the cytoplasm and the rest of the T2SS machinery.
CC       {ECO:0000250|UniProtKB:P25060}.
CC   -!- SUBUNIT: Type II secretion system is composed of four main components:
CC       the outer membrane complex, the inner membrane complex, the cytoplasmic
CC       secretion ATPase and the periplasm-spanning pseudopilus (By
CC       similarity). Forms homodimers. Interacts with PulM/GspM. Interacts with
CC       PulE/GspE and PulF/GspF (By similarity). {ECO:0000250|UniProtKB:P25060,
CC       ECO:0000250|UniProtKB:Q00514}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P25060}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P25060}.
CC   -!- SIMILARITY: Belongs to the GSP L family. {ECO:0000305}.
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DR   EMBL; M32613; AAA25134.1; -; Genomic_DNA.
DR   EMBL; X52462; CAA36697.1; -; Genomic_DNA.
DR   PIR; S11800; S11800.
DR   AlphaFoldDB; P15751; -.
DR   SMR; P15751; -.
DR   TCDB; 3.A.15.1.1; the outer membrane protein secreting main terminal branch (mtb) family.
DR   GO; GO:0009276; C:Gram-negative-bacterium-type cell wall; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR024230; GspL_cyto_dom.
DR   InterPro; IPR025691; GspL_pp_dom.
DR   InterPro; IPR007812; T2SS_protein-GspL.
DR   Pfam; PF12693; GspL_C; 1.
DR   Pfam; PF05134; T2SSL; 1.
DR   PIRSF; PIRSF015761; Protein_L; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR01709; typeII_sec_gspL; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..398
FT                   /note="Type II secretion system protein L"
FT                   /id="PRO_0000207317"
FT   TOPO_DOM        1..248
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25060"
FT   TRANSMEM        249..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000250|UniProtKB:P25060"
FT   TOPO_DOM        266..398
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25060"
SQ   SEQUENCE   398 AA;  44199 MW;  72CF5D870EA99948 CRC64;
     MNNHHTSSAA VLIIRLNPDA ATAIWRLAAP GDTAQTGEWH PDAGDPTLSL LAQRHPAWVL
     VPASDCAFHR VALPAGARRR PQQALAFLLE EQLATEVEES HFALLHQHKT DCAVAVVGRG
     KMRAWQAWCD SLGLSVLALT PDVLALPHSP TGWSAVRCGE QWLFRCDTWG GMAVETVWLD
     QLLTHWQDLA PIACYSPPPD IAAPWQPLPA QDLLQLAAAN PDARRICLRQ GDFAAKRRRQ
     PTPRRWRPVI VAALALLLLW SSNCLHDHLM LGQQADAAVQ ASRDFYRQWF QTEKNVVNPR
     LQMQQHLRQM KNAGARPALI SRLGALQQII DDTPGIRLRT LSFDAARNAL QLEISAVSSQ
     ALEQFSQRAR ARFRVQTGEM KPRADGIEGR LTLEGNDA