ID   GSPM_KLEPN              Reviewed;         161 AA.
AC   P15752;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Type II secretion system protein M;
DE            Short=T2SS protein M;
DE   AltName: Full=General secretion pathway protein M;
DE   AltName: Full=Pullulanase secretion protein PulM;
GN   Name=pulM;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UNF 5023;
RX   PubMed=2162463; DOI=10.1111/j.1365-2958.1990.tb00604.x;
RA   Pugsley A.P., Reyss I.;
RT   "Five genes at the 3' end of the Klebsiella pneumoniae pulC operon are
RT   required for pullulanase secretion.";
RL   Mol. Microbiol. 4:365-379(1990).
CC   -!- FUNCTION: Inner membrane component of the type II secretion system
CC       required for the energy-dependent secretion of extracellular factors
CC       such as proteases and toxins from the periplasm. Plays a role in the
CC       complex assembly and recruits PulL resulting in a stable complex in the
CC       inner membrane. Provides thus a link between the energy-providing PulE
CC       protein in the cytoplasm and the rest of the T2SS machinery.
CC       {ECO:0000250|UniProtKB:P25061}.
CC   -!- SUBUNIT: Type II secretion system is composed of four main components:
CC       the outer membrane complex, the inner membrane complex, the cytoplasmic
CC       secretion ATPase and the periplasm-spanning pseudopilus (By
CC       similarity). Forms homodimers (By similarity). Interacts with
CC       PulL/GspL. Interacts with PulE/GspE and PulF/GspF (By similarity).
CC       {ECO:0000250|UniProtKB:P25061, ECO:0000250|UniProtKB:P41851,
CC       ECO:0000250|UniProtKB:Q00514}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P25061}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P25061}.
CC   -!- SIMILARITY: Belongs to the GSP M family. {ECO:0000305}.
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DR   EMBL; M32613; AAA25135.1; -; Genomic_DNA.
DR   EMBL; X52462; CAA36698.1; -; Genomic_DNA.
DR   PIR; S11801; S11801.
DR   AlphaFoldDB; P15752; -.
DR   SMR; P15752; -.
DR   TCDB; 3.A.15.1.1; the outer membrane protein secreting main terminal branch (mtb) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR007690; T2SS_GspM.
DR   InterPro; IPR023229; T2SS_M_periplasmic_sf.
DR   Pfam; PF04612; T2SSM; 1.
DR   PIRSF; PIRSF006291; GspM; 1.
DR   SUPFAM; SSF103054; SSF103054; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..161
FT                   /note="Type II secretion system protein M"
FT                   /id="PRO_0000207326"
FT   TOPO_DOM        1..16
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25061"
FT   TRANSMEM        17..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..161
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25061"
SQ   SEQUENCE   161 AA;  18419 MW;  D3C8BE3F963DF700 CRC64;
     MHNLLALWQQ RTRRERCLLL GMAVVLLIGL VYYTLWQPWQ NREAQWRQTL AREQASLQWM
     RQQTPLIRQL RNQKPPTAPE EPSTVIMREA ARHGLTIVRL QPQGSRLSLT VQPADFQALM
     AWLDALGQAG MTTATLAVTA VAQQPGWVTV NTLVLERSDE K