ID   GSPM_PECCC              Reviewed;         164 AA.
AC   P31709;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Type II secretion system protein M;
DE            Short=T2SS protein M;
DE   AltName: Full=General secretion pathway protein M;
DE   AltName: Full=Pectic enzymes secretion protein OutM;
GN   Name=outM;
OS   Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp.
OS   carotovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=555;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SCRI 193;
RX   PubMed=8326859; DOI=10.1111/j.1365-2958.1993.tb01589.x;
RA   Reeves P.J., Whitcombe D., Wharam S., Gibson M., Allison G., Bunce N.,
RA   Barallon R., Douglas P., Mulholland V., Stevens S., Walker S.,
RA   Salmond G.P.C.;
RT   "Molecular cloning and characterization of 13 out genes from Erwinia
RT   carotovora subspecies carotovora: genes encoding members of a general
RT   secretion pathway (GSP) widespread in Gram-negative bacteria.";
RL   Mol. Microbiol. 8:443-456(1993).
CC   -!- FUNCTION: Inner membrane component of the type II secretion system
CC       required for the energy-dependent secretion of extracellular factors
CC       such as proteases and toxins from the periplasm. Plays a role in the
CC       complex assembly and recruits OutL resulting in a stable complex in the
CC       inner membrane. Provides thus a link between the energy-providing OutE
CC       protein in the cytoplasm and the rest of the T2SS machinery.
CC       {ECO:0000250|UniProtKB:P25061}.
CC   -!- SUBUNIT: Type II secretion system is composed of four main components:
CC       the outer membrane complex, the inner membrane complex, the cytoplasmic
CC       secretion ATPase and the periplasm-spanning pseudopilus (By
CC       similarity). Forms homodimers (By similarity). Interacts with
CC       OutL/GspL. Interacts with OutE/GspE and OutF/GspF (By similarity).
CC       {ECO:0000250|UniProtKB:P25061, ECO:0000250|UniProtKB:P41851,
CC       ECO:0000250|UniProtKB:Q00514}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P25061}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P25061}.
CC   -!- SIMILARITY: Belongs to the GSP M family. {ECO:0000305}.
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DR   EMBL; X70049; CAA49654.1; -; Genomic_DNA.
DR   PIR; S32867; S32867.
DR   AlphaFoldDB; P31709; -.
DR   SMR; P31709; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR007690; T2SS_GspM.
DR   InterPro; IPR023229; T2SS_M_periplasmic_sf.
DR   Pfam; PF04612; T2SSM; 1.
DR   PIRSF; PIRSF006291; GspM; 1.
DR   SUPFAM; SSF103054; SSF103054; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..164
FT                   /note="Type II secretion system protein M"
FT                   /id="PRO_0000207324"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25061"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        39..164
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P25061"
SQ   SEQUENCE   164 AA;  18771 MW;  27591A4E8AB221CD CRC64;
     MNELRQRWQA MSQRERQLMV VCAAVLLLCV VYYAILQPWQ EREDLWERTI SREQQTVNWM
     QKQASSIPRG NQAQGDNSQR DVSLPILISQ STKRYGLTVV RLQPQGSQAS VTLGQSDFNS
     LLRWLGELEQ KNGVKVISLD VNAVEQSPGI VDVTRLMLER TDEA