ID   GSPN_KLEPN              Reviewed;         252 AA.
AC   P15753;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Type II secretion system protein N;
DE            Short=T2SS protein N;
DE   AltName: Full=General secretion pathway protein N;
DE   AltName: Full=Pullulanase secretion protein PulN;
GN   Name=pulN;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UNF 5023;
RX   PubMed=2162463; DOI=10.1111/j.1365-2958.1990.tb00604.x;
RA   Pugsley A.P., Reyss I.;
RT   "Five genes at the 3' end of the Klebsiella pneumoniae pulC operon are
RT   required for pullulanase secretion.";
RL   Mol. Microbiol. 4:365-379(1990).
CC   -!- FUNCTION: Involved in a type II secretion system (T2SS, formerly
CC       general secretion pathway, GSP) for the export of proteins (By
CC       similarity). Required for the translocation of pullulanase.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GSP N family. {ECO:0000305}.
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DR   EMBL; M32613; AAA25136.1; -; Genomic_DNA.
DR   EMBL; X52462; CAA36699.1; -; Genomic_DNA.
DR   PIR; S11802; S11802.
DR   AlphaFoldDB; P15753; -.
DR   TCDB; 3.A.15.1.1; the outer membrane protein secreting main terminal branch (mtb) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR000645; T2SS_GspN_CS.
DR   InterPro; IPR022792; T2SS_protein-GspN.
DR   Pfam; PF01203; T2SSN; 1.
DR   PROSITE; PS01142; T2SP_N; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..252
FT                   /note="Type II secretion system protein N"
FT                   /id="PRO_0000195054"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..252
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   252 AA;  27694 MW;  A443640FCADF60C2 CRC64;
     MKNRLTIGLL LAAIYLFWLL LSAPARLLAL TLSDDARLAQ TSGTLWQGEA HQASWRGVEL
     AYLRWEFGFS TWLPGWHIRF NDPSGLRGQA WLHGLNEFVV REGRLVIPAR LISQRLALGM
     PLEARGQLAL TLPEASFNAN GCRRIAASAV QWQDAALSSP AGLLELAQVN GKLSCTPAGA
     LAVALTQDSH QLSLTGQGVL TPDGRYTFNG TLQPRQAAPA LLTLLVAQNG RKDEQGRIPW
     RWQGEWLSEE KK