ID   GSPN_VIBCH              Reviewed;         252 AA.
AC   P45784; Q9KNK9;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Type II secretion system protein N;
DE            Short=T2SS protein N;
DE   AltName: Full=Cholera toxin secretion protein EpsN;
DE   AltName: Full=General secretion pathway protein N;
GN   Name=epsN; OrderedLocusNames=VC_2723;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor TRH7000;
RA   Overbye L.J.;
RT   "Organization of the general secretion pathway genes in Vibrio cholerae.";
RL   Thesis (1994), Michigan State University, United States.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Involved in a type II secretion system (T2SS, formerly
CC       general secretion pathway, GSP) for the export of proteins (By
CC       similarity). Required for secretion of cholera toxin through the outer
CC       membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GSP N family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L33796; AAA58795.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF95863.1; -; Genomic_DNA.
DR   PIR; E82040; E82040.
DR   PIR; PN0647; PN0647.
DR   RefSeq; NP_232350.1; NC_002505.1.
DR   RefSeq; WP_000817146.1; NZ_LT906614.1.
DR   AlphaFoldDB; P45784; -.
DR   STRING; 243277.VC_2723; -.
DR   DNASU; 2615551; -.
DR   EnsemblBacteria; AAF95863; AAF95863; VC_2723.
DR   GeneID; 57741315; -.
DR   KEGG; vch:VC_2723; -.
DR   PATRIC; fig|243277.26.peg.2598; -.
DR   eggNOG; ENOG5032RTB; Bacteria.
DR   HOGENOM; CLU_092754_1_0_6; -.
DR   OMA; NDIAFEG; -.
DR   BioCyc; VCHO:VC2723-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR   GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR   InterPro; IPR000645; T2SS_GspN_CS.
DR   InterPro; IPR022792; T2SS_protein-GspN.
DR   Pfam; PF01203; T2SSN; 1.
DR   PROSITE; PS01142; T2SP_N; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..252
FT                   /note="Type II secretion system protein N"
FT                   /id="PRO_0000195056"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..252
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        155..164
FT                   /note="GSLAWSAAQL -> ELSLVSRAV (in Ref. 1; AAA58795)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   252 AA;  27354 MW;  8A96A61460CA0AB8 CRC64;
     MKRAVGYGLL FSTVLMTSVV VHLPAQVALS PLPLPEGLEL TGIEGTLWQG QAAQVRWQGM
     SLGDLNWDLH LSALLLGQLE ADIRFGRGSS TQLRGKGVVG VGLSGPYADD FLLSLPAAQA
     ITWLPLPVPL MAQGQLEMAV KQYRFGEPYC QQAEGSLAWS AAQLESPIGA LQLGTVVSDF
     TCQESVVTLK GGQKTAQVSS EFNLSLQPDN RYQAQAWFKP EAEFPESLKE QLSWLPQPDG
     QGRYPFNQQG QL