GST1_BLAGE
ID GST1_BLAGE Reviewed; 204 AA.
AC O18598;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Glutathione S-transferase;
DE EC=2.5.1.18;
DE AltName: Full=GST class-sigma;
DE AltName: Full=Major allergen Bla g 5;
DE AltName: Allergen=Bla g 5;
OS Blattella germanica (German cockroach) (Blatta germanica).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blaberoidea; Ectobiidae;
OC Blattellinae; Blattella.
OX NCBI_TaxID=6973;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-204, AND PROTEIN SEQUENCE OF 2-39.
RX PubMed=9252418; DOI=10.1074/jbc.272.33.20907;
RA Arruda L.K., Vailes L.D., Platts-Mills T.A.E., Hayden M.L., Chapman M.D.;
RT "Induction of IgE antibody responses by glutathione S-transferase from the
RT German cockroach (Blattella germanica).";
RL J. Biol. Chem. 272:20907-20912(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutathione + RX = a halide anion + an S-substituted
CC glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC ChEBI:CHEBI:90779; EC=2.5.1.18;
CC -!- ALLERGEN: Causes an allergic reaction in human. Causes positive
CC immediate skin tests in cockroach-allergic patients using as little as
CC 3 pg of recombinant protein. Associated with asthma.
CC -!- SIMILARITY: Belongs to the GST superfamily. Sigma family.
CC {ECO:0000305}.
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DR EMBL; U92412; AAB72147.1; -; mRNA.
DR PDB; 4Q5R; X-ray; 2.25 A; A/B/C/D/E/F=1-204.
DR PDBsum; 4Q5R; -.
DR AlphaFoldDB; O18598; -.
DR SMR; O18598; -.
DR Allergome; 144; Bla g 5.
DR Allergome; 3142; Bla g 5.0101.
DR GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR040079; Glutathione_S-Trfase.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR004046; GST_C.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR Pfam; PF14497; GST_C_3; 1.
DR Pfam; PF02798; GST_N; 1.
DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Transferase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9252418"
FT CHAIN 2..204
FT /note="Glutathione S-transferase"
FT /id="PRO_0000185916"
FT DOMAIN 3..80
FT /note="GST N-terminal"
FT DOMAIN 82..204
FT /note="GST C-terminal"
FT BINDING 9
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250|UniProtKB:O60760"
FT BINDING 40
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250|UniProtKB:O60760"
FT BINDING 44
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250|UniProtKB:P46088"
FT BINDING 50..52
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250|UniProtKB:O60760"
FT BINDING 64..65
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250|UniProtKB:O60760"
FT CONFLICT 10
FT /note="C -> F (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 5..9
FT /evidence="ECO:0007829|PDB:4Q5R"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:4Q5R"
FT TURN 14..16
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 17..25
FT /evidence="ECO:0007829|PDB:4Q5R"
FT STRAND 31..34
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 40..43
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:4Q5R"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:4Q5R"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 65..75
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 83..107
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 112..124
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 126..140
FT /evidence="ECO:0007829|PDB:4Q5R"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 152..168
FT /evidence="ECO:0007829|PDB:4Q5R"
FT TURN 172..175
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 177..188
FT /evidence="ECO:0007829|PDB:4Q5R"
FT HELIX 190..198
FT /evidence="ECO:0007829|PDB:4Q5R"
SQ SEQUENCE 204 AA; 23334 MW; 4CBE9339C913551C CRC64;
MAPSYKLTYC PVKALGEPIR FLLSYGEKDF EDYRFQEGDW PNLKPSMPFG KTPVLEIDGK
QTHQSVAISR YLGKQFGLSG KDDWENLEID MIVDTISDFR AAIANYHYDA DENSKQKKWD
PLKKETIPYY TKKFDEVVKA NGGYLAAGKL TWADFYFVAI LDYLNHMAKE DLVANQPNLK
ALREKVLGLP AIKAWVAKRP PTDL
