GSTT3_ARATH
ID GSTT3_ARATH Reviewed; 590 AA.
AC Q9FHE1; Q56WE4;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Glutathione S-transferase T3;
DE Short=AtGSTT3;
DE EC=2.5.1.18;
DE AltName: Full=GST class-theta member 3;
DE AltName: Full=Glutathione S-transferase 10C;
GN Name=GSTT3; Synonyms=GST10C; OrderedLocusNames=At5g41220; ORFNames=K1O13.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 249-590.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=19174456; DOI=10.1093/jxb/ern365;
RA Dixon D.P., Hawkins T., Hussey P.J., Edwards R.;
RT "Enzyme activities and subcellular localization of members of the
RT Arabidopsis glutathione transferase superfamily.";
RL J. Exp. Bot. 60:1207-1218(2009).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12090627; DOI=10.1023/a:1015557300450;
RA Wagner U., Edwards R., Dixon D.P., Mauch F.;
RT "Probing the diversity of the Arabidopsis glutathione S-transferase gene
RT family.";
RL Plant Mol. Biol. 49:515-532(2002).
CC -!- FUNCTION: May be involved in the conjugation of reduced glutathione to
CC a wide number of exogenous and endogenous hydrophobic electrophiles and
CC have a detoxification role against certain herbicides. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutathione + RX = a halide anion + an S-substituted
CC glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC ChEBI:CHEBI:90779; EC=2.5.1.18;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19174456}.
CC -!- SIMILARITY: Belongs to the GST superfamily. Theta family.
CC {ECO:0000305}.
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DR EMBL; AB019225; BAB11098.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94655.1; -; Genomic_DNA.
DR EMBL; AK222098; BAD95009.1; -; mRNA.
DR RefSeq; NP_198938.1; NM_123487.4.
DR AlphaFoldDB; Q9FHE1; -.
DR SMR; Q9FHE1; -.
DR STRING; 3702.AT5G41220.1; -.
DR PaxDb; Q9FHE1; -.
DR PRIDE; Q9FHE1; -.
DR ProteomicsDB; 247187; -.
DR EnsemblPlants; AT5G41220.1; AT5G41220.1; AT5G41220.
DR GeneID; 834124; -.
DR Gramene; AT5G41220.1; AT5G41220.1; AT5G41220.
DR KEGG; ath:AT5G41220; -.
DR Araport; AT5G41220; -.
DR TAIR; locus:2155090; AT5G41220.
DR eggNOG; KOG0867; Eukaryota.
DR HOGENOM; CLU_462617_0_0_1; -.
DR InParanoid; Q9FHE1; -.
DR OrthoDB; 1231780at2759; -.
DR PhylomeDB; Q9FHE1; -.
DR BioCyc; ARA:AT5G41220-MON; -.
DR PRO; PR:Q9FHE1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FHE1; baseline and differential.
DR Genevisible; Q9FHE1; AT.
DR GO; GO:0005737; C:cytoplasm; NAS:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR GO; GO:0009407; P:toxin catabolic process; TAS:TAIR.
DR CDD; cd03183; GST_C_Theta; 1.
DR CDD; cd03050; GST_N_Theta; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR040077; GST_C_Theta.
DR InterPro; IPR040075; GST_N_Theta.
DR InterPro; IPR043377; GSTT1/2/3.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR44750; PTHR44750; 1.
DR Pfam; PF02798; GST_N; 1.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
DR PROSITE; PS50090; MYB_LIKE; 1.
PE 2: Evidence at transcript level;
KW Detoxification; Nucleus; Reference proteome; Transferase.
FT CHAIN 1..590
FT /note="Glutathione S-transferase T3"
FT /id="PRO_0000413576"
FT DOMAIN 1..82
FT /note="GST N-terminal"
FT DOMAIN 89..232
FT /note="GST C-terminal"
FT DOMAIN 265..336
FT /note="Myb-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT REGION 402..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 11..12
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 40..41
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 53..54
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 66..67
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
SQ SEQUENCE 590 AA; 67555 MW; E85F66525F6C7AF5 CRC64;
MKLKVYADRM SQPSRAVLIF CKVNEIQFDE ILIYLANRQQ LSPEFKDINP MGKVPAIVDG
KLKLSESHAI LIYLSSAYPS VVDHWYPTDL SKRARIHSVL DWHHTNLRPG AAGYVLNSVL
GPALGLPLNP KAAAEAEQLL TKSLTTLDTF WLKGNAMFLL GSNQPSIADL SLVCELTQLQ
VLDDKDRLRL LSPHKNVEQW IENTRKATMP HFDEVHEVLF RAKDRCQKQR EMATASKPGP
QSKIIQFSTI GEKSDDPNLV QNTTDRRKHR RKWSRAEDAI LISAWLNTSK DPIVDNEHKA
CAFWKRIGAY FNNSASLANL PKREPSHCKQ RWSKLNDKVC KFVGCYDQAL NQRSSGQSED
DVFQVAYQVY TNNYKSNFTL EHAWRELRHS KKWCSLYPFE NSKGGGSSKR TKLNNGDRVY
SSSSNPESVP IALDEEEQVM DLPLGVKSSK QKEKKVATII TIEEREADSG SRLENLWVLD
EEEQVMDRPL GVKSLEQKEN KVAPKPTIEE REAADSRSRL ENLWALKEKE EREADSRSRL
ENLWALKEKD IEEQKKLTRM EVLKSLLGRT TDQLSEKEDI LKNKLIDEML
