GSTU6_ORYSJ
ID GSTU6_ORYSJ Reviewed; 236 AA.
AC Q06398; B7E411; Q0IW60; Q9FUE6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable glutathione S-transferase GSTU6;
DE EC=2.5.1.18;
DE AltName: Full=28 kDa cold-induced protein;
GN Name=GSTU6; OrderedLocusNames=Os10g0530900, LOC_Os10g38740;
GN ORFNames=OSJNBb0038A07.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seedling;
RX PubMed=16668981; DOI=10.1104/pp.99.3.1146;
RA Binh L.T., Oono K.;
RT "Molecular cloning and characterization of genes related to chilling
RT tolerance in rice.";
RL Plant Physiol. 99:1146-1150(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND NOMENCLATURE.
RX PubMed=15069639; DOI=10.1007/s00438-004-1006-8;
RA Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.;
RT "Organisation and structural evolution of the rice glutathione S-
RT transferase gene family.";
RL Mol. Genet. Genomics 271:511-521(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC exogenous and endogenous hydrophobic electrophiles.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutathione + RX = a halide anion + an S-substituted
CC glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC ChEBI:CHEBI:90779; EC=2.5.1.18;
CC -!- TISSUE SPECIFICITY: Expressed in seedling shoots and roots.
CC -!- INDUCTION: By cold stress.
CC -!- SIMILARITY: Belongs to the GST superfamily. Tau family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA01632.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D10861; BAA01632.1; ALT_FRAME; mRNA.
DR EMBL; AF309376; AAG32469.1; -; mRNA.
DR EMBL; AC113948; AAM94535.1; -; Genomic_DNA.
DR EMBL; DP000086; AAP54769.1; -; Genomic_DNA.
DR EMBL; AP008216; BAF27055.1; -; Genomic_DNA.
DR EMBL; AP014966; BAT11780.1; -; Genomic_DNA.
DR EMBL; AK059760; BAG87108.1; -; mRNA.
DR EMBL; AK103699; BAG96210.1; -; mRNA.
DR RefSeq; XP_015614356.1; XM_015758870.1.
DR AlphaFoldDB; Q06398; -.
DR SMR; Q06398; -.
DR STRING; 4530.OS10T0530900-01; -.
DR PaxDb; Q06398; -.
DR PRIDE; Q06398; -.
DR EnsemblPlants; Os10t0530900-01; Os10t0530900-01; Os10g0530900.
DR GeneID; 4349207; -.
DR Gramene; Os10t0530900-01; Os10t0530900-01; Os10g0530900.
DR KEGG; osa:4349207; -.
DR eggNOG; KOG0406; Eukaryota.
DR HOGENOM; CLU_011226_18_0_1; -.
DR InParanoid; Q06398; -.
DR OMA; MADSNVK; -.
DR OrthoDB; 1225872at2759; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR ExpressionAtlas; Q06398; baseline and differential.
DR Genevisible; Q06398; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR CDD; cd03185; GST_C_Tau; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR040079; Glutathione_S-Trfase.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR045074; GST_C_Tau.
DR InterPro; IPR045073; Omega/Tau-like.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR11260; PTHR11260; 1.
DR Pfam; PF13417; GST_N_3; 1.
DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Stress response; Transferase.
FT CHAIN 1..236
FT /note="Probable glutathione S-transferase GSTU6"
FT /id="PRO_0000185870"
FT DOMAIN 5..84
FT /note="GST N-terminal"
FT DOMAIN 94..228
FT /note="GST C-terminal"
FT BINDING 15
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 42
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 56
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 68..69
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT CONFLICT 65
FT /note="Missing (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 83..88
FT /note="GAGGRP -> RAGR (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 99..103
FT /note="AVARF -> VL (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="Missing (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 125
FT /note="T -> M (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 135..143
FT /note="QAVAALETL -> RRGGVETV (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 158..162
FT /note="GGDGV -> ARRR (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="V -> VV (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="Missing (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 194..195
FT /note="PA -> SS (in Ref. 1; BAA01632)"
FT /evidence="ECO:0000305"
FT CONFLICT 203..216
FT /note="FRATDAAKGVVPDD -> STHRLQCVDVPMT (in Ref. 1;
FT BAA01632)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 236 AA; 25656 MW; 60A5299744CB1F6C CRC64;
MAGSGELKLL GVWSSPYAIR VRVVLNLKSL PYEYVEENLG DKSDLLLASN PVHKSVPVLL
HAGRPVNESQ VIVQYIDEVW PGGAGGRPSV MPSDPYERAV ARFWAAYVDD KVRPAWLAIL
FGSKTEEERA AAVAQAVAAL ETLEGAFGEC SKGKPFFGGD GVGFVDVVLG GYLGWFTAID
KLIGRRLIDP ARTPALAAWE ERFRATDAAK GVVPDDADKL LEFRQTLLRW SASKAK
