GSTU9_ARATH
ID GSTU9_ARATH Reviewed; 240 AA.
AC Q9FUT0; Q9FE97;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Glutathione S-transferase U9;
DE Short=AtGSTU9;
DE EC=2.5.1.18;
DE AltName: Full=GST class-tau member 9;
DE AltName: Full=Glutathione S-transferase 14;
GN Name=GSTU9; Synonyms=GST14, GST14B; OrderedLocusNames=At5g62480;
GN ORFNames=K19B1.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12090627; DOI=10.1023/a:1015557300450;
RA Wagner U., Edwards R., Dixon D.P., Mauch F.;
RT "Probing the diversity of the Arabidopsis glutathione S-transferase gene
RT family.";
RL Plant Mol. Biol. 49:515-532(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the conjugation of reduced glutathione to
CC a wide number of exogenous and endogenous hydrophobic electrophiles and
CC have a detoxification role against certain herbicides. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glutathione + RX = a halide anion + an S-substituted
CC glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC ChEBI:CHEBI:90779; EC=2.5.1.18;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FUT0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FUT0-2; Sequence=VSP_041940;
CC -!- SIMILARITY: Belongs to the GST superfamily. Tau family. {ECO:0000305}.
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DR EMBL; AF288179; AAG30128.1; -; mRNA.
DR EMBL; AF288180; AAG30129.1; -; mRNA.
DR EMBL; AB015469; BAB11498.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97612.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97613.1; -; Genomic_DNA.
DR EMBL; AK176211; BAD43974.1; -; mRNA.
DR RefSeq; NP_568954.2; NM_125642.3. [Q9FUT0-2]
DR RefSeq; NP_851249.1; NM_180918.3. [Q9FUT0-1]
DR AlphaFoldDB; Q9FUT0; -.
DR SMR; Q9FUT0; -.
DR STRING; 3702.AT5G62480.1; -.
DR PaxDb; Q9FUT0; -.
DR ProteomicsDB; 248496; -. [Q9FUT0-1]
DR EnsemblPlants; AT5G62480.1; AT5G62480.1; AT5G62480. [Q9FUT0-1]
DR EnsemblPlants; AT5G62480.2; AT5G62480.2; AT5G62480. [Q9FUT0-2]
DR GeneID; 836368; -.
DR Gramene; AT5G62480.1; AT5G62480.1; AT5G62480. [Q9FUT0-1]
DR Gramene; AT5G62480.2; AT5G62480.2; AT5G62480. [Q9FUT0-2]
DR KEGG; ath:AT5G62480; -.
DR Araport; AT5G62480; -.
DR TAIR; locus:2154129; AT5G62480.
DR eggNOG; KOG0406; Eukaryota.
DR HOGENOM; CLU_011226_18_0_1; -.
DR InParanoid; Q9FUT0; -.
DR OMA; CKAQEEV; -.
DR OrthoDB; 1225872at2759; -.
DR PhylomeDB; Q9FUT0; -.
DR BioCyc; ARA:AT5G62480-MON; -.
DR BRENDA; 2.5.1.18; 399.
DR PRO; PR:Q9FUT0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FUT0; baseline and differential.
DR Genevisible; Q9FUT0; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR GO; GO:0009407; P:toxin catabolic process; TAS:TAIR.
DR CDD; cd03185; GST_C_Tau; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR045074; GST_C_Tau.
DR InterPro; IPR045073; Omega/Tau-like.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR11260; PTHR11260; 1.
DR Pfam; PF13417; GST_N_3; 1.
DR SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Detoxification; Phosphoprotein;
KW Reference proteome; Transferase.
FT CHAIN 1..240
FT /note="Glutathione S-transferase U9"
FT /id="PRO_0000413555"
FT DOMAIN 7..86
FT /note="GST N-terminal"
FT DOMAIN 92..226
FT /note="GST C-terminal"
FT BINDING 17..18
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 43..44
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 57..58
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT BINDING 70..71
FT /ligand="glutathione"
FT /ligand_id="ChEBI:CHEBI:57925"
FT /evidence="ECO:0000250"
FT MOD_RES 161
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ZW27"
FT VAR_SEQ 65..90
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12090627"
FT /id="VSP_041940"
SQ SEQUENCE 240 AA; 27612 MW; D0872464356EA2FB CRC64;
MDEEVENKVI LHGSFASPYS KRIELALRLK SIPYQFVQED LQNKSQTLLR YNPVHKKIPV
LVHNGKPISE SLFIIEYIDE TWSNGPHILP EDPYRRSKVR FWANYIQLHL YDLVIKVVKS
EGEEQKKALT EVKEKLSVIE KEGLKEIFSD TDGEPTVTNE TMSLVDIVMC TLLSPYKAHE
EVLGLKIIDP EIVPGVYGWI NAINETSVVK DLSPPYEQIL EILRAFRQMS LSPVLETYQS
