ID   GSTX1_NICPL             Reviewed;         219 AA.
AC   P50471;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Probable glutathione S-transferase MSR-1;
DE            EC=2.5.1.18;
DE   AltName: Full=Auxin-regulated protein MSR-1;
GN   Name=MSR-1;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1498603; DOI=10.2307/3869446;
RA   Dominov J.A., Stenzler L., Lee S., Schwarz J.J., Leisner S., Howell S.H.;
RT   "Cytokinins and auxins control the expression of a gene in Nicotiana
RT   plumbaginifolia cells by feedback regulation.";
RL   Plant Cell 4:451-461(1992).
CC   -!- FUNCTION: May play an important role in hormonal and growth regulatory
CC       responses.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- INDUCTION: By auxin and cytokinin.
CC   -!- SIMILARITY: Belongs to the GST superfamily. HSP26 family.
CC       {ECO:0000305}.
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DR   EMBL; S44036; AAB47712.2; -; mRNA.
DR   PIR; JQ1606; JQ1606.
DR   AlphaFoldDB; P50471; -.
DR   SMR; P50471; -.
DR   GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR004046; GST_C.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260; PTHR11260; 1.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; Transferase.
FT   CHAIN           1..219
FT                   /note="Probable glutathione S-transferase MSR-1"
FT                   /id="PRO_0000185862"
FT   DOMAIN          4..83
FT                   /note="GST N-terminal"
FT   DOMAIN          89..208
FT                   /note="GST C-terminal"
FT   BINDING         14
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         67..68
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   219 AA;  25269 MW;  DCE165B0FB33A759 CRC64;
     MESNNVVLLD FSGSSFGMRL RIALALKGIK YEAKEENLSD KSPLLLEMNP VHKKIPILIH
     NGKPICESLN ILEYIDEVWH EKCPLLPSDP YQRSQARFWA NYIDNKIYST GRRVWSGKGE
     DQEEAKKEFI EIFKTLEGEL GNKTYFGGDN LGFVDVALVP FTSWFYSYET CANFSIEAEC
     RKLVVWQNCM ENERVSKSLP HPHKIYDFVL ELKHKLGLA