ID   GSTX2_TOBAC             Reviewed;         223 AA.
AC   Q03663;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Probable glutathione S-transferase;
DE            EC=2.5.1.18;
DE   AltName: Full=Auxin-induced protein PGNT35/PCNT111;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Samsun NN, and cv. White Burley; TISSUE=Leaf;
RX   PubMed=1863770; DOI=10.1007/bf00016071;
RA   van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C.,
RA   Schilperoort R.A., Libbenga K.R.;
RT   "Promoters of auxin-induced genes from tobacco can lead to auxin-inducible
RT   and root tip-specific expression.";
RL   Plant Mol. Biol. 16:983-998(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- TISSUE SPECIFICITY: Root tip-specific expression.
CC   -!- INDUCTION: By auxin.
CC   -!- SIMILARITY: Belongs to the GST superfamily. HSP26 family.
CC       {ECO:0000305}.
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DR   EMBL; X56269; CAA39710.1; -; Genomic_DNA.
DR   EMBL; X56265; CAA39706.1; -; mRNA.
DR   PIR; S16268; S16268.
DR   RefSeq; NP_001312621.1; NM_001325692.1.
DR   AlphaFoldDB; Q03663; -.
DR   SMR; Q03663; -.
DR   STRING; 4097.Q03663; -.
DR   GeneID; 107800866; -.
DR   KEGG; nta:107800866; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR004046; GST_C.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260; PTHR11260; 1.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; Reference proteome; Transferase.
FT   CHAIN           1..223
FT                   /note="Probable glutathione S-transferase"
FT                   /id="PRO_0000185867"
FT   DOMAIN          2..81
FT                   /note="GST N-terminal"
FT   DOMAIN          86..212
FT                   /note="GST C-terminal"
FT   BINDING         12
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         39
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         53
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         65..66
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   223 AA;  25842 MW;  ACD523DB2A003A83 CRC64;
     MAEVKLLGFW YSPFSHRVEW ALKIKGVKYE YIEEDRDNKS SLLLQSNPVY KKVPVLIHNG
     KPIVESMIIL EYIDETFEGP SILPKDPYDR ALARFWAKFL DDKVAAVVNT FFRKGEEQEK
     GKEEVYEMLK VLDNELKDKK FFAGDKFGFA DIAANLVGFW LGVFEEGYGD VLVKSEKFPN
     FSKWRDEYIN CSQVNESLPP RDELLAFFRA RFQAVVASRS APK