ID   GSTX4_TOBAC             Reviewed;         221 AA.
AC   Q03666;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Probable glutathione S-transferase;
DE            EC=2.5.1.18;
DE   AltName: Full=Auxin-induced protein PCNT107;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. White Burley;
RX   PubMed=1863770; DOI=10.1007/bf00016071;
RA   van der Zaal E.J., Droog F.N.J., Boot C.J.M., Hensgens L.A.M., Hoge J.H.C.,
RA   Schilperoort R.A., Libbenga K.R.;
RT   "Promoters of auxin-induced genes from tobacco can lead to auxin-inducible
RT   and root tip-specific expression.";
RL   Plant Mol. Biol. 16:983-998(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- TISSUE SPECIFICITY: Root tip-specific expression.
CC   -!- INDUCTION: By auxin.
CC   -!- SIMILARITY: Belongs to the GST superfamily. HSP26 family.
CC       {ECO:0000305}.
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DR   EMBL; X56266; CAA39707.1; -; mRNA.
DR   PIR; S16636; S16636.
DR   RefSeq; NP_001312277.1; NM_001325348.1.
DR   AlphaFoldDB; Q03666; -.
DR   SMR; Q03666; -.
DR   STRING; 4097.Q03666; -.
DR   GeneID; 107782951; -.
DR   KEGG; nta:107782951; -.
DR   OMA; WVNACLE; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260; PTHR11260; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; Reference proteome; Transferase.
FT   CHAIN           1..221
FT                   /note="Probable glutathione S-transferase"
FT                   /id="PRO_0000185865"
FT   DOMAIN          4..83
FT                   /note="GST N-terminal"
FT   DOMAIN          90..214
FT                   /note="GST C-terminal"
FT   BINDING         14
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         67..68
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  25789 MW;  1488D87B93BC4C60 CRC64;
     MANEEVILLD FWPSMFGMRL RIALAEKEIK YEYKEEDLRN KSPLLLQMNP IHKKIPVLIH
     NGKPICESII AVEYIEEVWK DKAPNLLPSD PYDRAQARFW ADYIDKKLYD FGRKLWTTKG
     EEQEAAKKDF IECLKVLEGA LGDKPYFGGE SFGFVDIALI GYYSWFYAYE TFGNFSTEAE
     CPKFVAWAKR CMQRESVAKS LPDQPKVLEF VKVLRQKFGL E