ID   GSTXA_TOBAC             Reviewed;         220 AA.
AC   P25317;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Probable glutathione S-transferase parA;
DE            EC=2.5.1.18;
DE   AltName: Full=Auxin-regulated protein parA;
DE   AltName: Full=STR246C protein;
GN   Name=PARA; Synonyms=PAR;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf mesophyll;
RX   PubMed=2594768; DOI=10.1073/pnas.86.23.9279;
RA   Takahashi Y., Kuroda H., Tanaka T., Machida Y., Takebe I., Nagata T.;
RT   "Isolation of an auxin-regulated gene cDNA expressed during the transition
RT   from G0 to S phase in tobacco mesophyll protoplasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:9279-9283(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. NK 326;
RX   PubMed=7948901; DOI=10.1007/bf00039563;
RA   Froissard D., Gough C., Czernic P., Schneider M., Toppan A., Roby D.,
RA   Marco Y.;
RT   "Structural organization of str 246C and str 246N, plant defense-related
RT   genes from Nicotiana tabacum.";
RL   Plant Mol. Biol. 26:515-521(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106.
RX   PubMed=2236015; DOI=10.1073/pnas.87.20.8013;
RA   Takahashi Y., Niwa Y., Machida Y., Nagata T.;
RT   "Location of the cis-acting auxin-responsive region in the promoter of the
RT   par gene from tobacco mesophyll protoplasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8013-8016(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- INDUCTION: By auxin.
CC   -!- SIMILARITY: Belongs to the GST superfamily. HSP26 family.
CC       {ECO:0000305}.
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DR   EMBL; D90215; BAA14243.1; -; Genomic_DNA.
DR   EMBL; M29274; AAA67894.1; -; mRNA.
DR   EMBL; X80829; CAA56790.1; -; Genomic_DNA.
DR   EMBL; X80828; CAA56789.1; -; mRNA.
DR   PIR; A36225; A36225.
DR   RefSeq; NP_001312067.1; NM_001325138.1.
DR   AlphaFoldDB; P25317; -.
DR   SMR; P25317; -.
DR   STRING; 4097.P25317; -.
DR   GeneID; 107772524; -.
DR   KEGG; nta:107772524; -.
DR   PhylomeDB; P25317; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   CDD; cd03185; GST_C_Tau; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR004046; GST_C.
DR   InterPro; IPR045074; GST_C_Tau.
DR   InterPro; IPR045073; Omega/Tau-like.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11260; PTHR11260; 1.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SFLD; SFLDG01152; Main.3:_Omega-_and_Tau-like; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; Reference proteome; Transferase.
FT   CHAIN           1..220
FT                   /note="Probable glutathione S-transferase parA"
FT                   /id="PRO_0000185863"
FT   DOMAIN          4..83
FT                   /note="GST N-terminal"
FT   DOMAIN          89..209
FT                   /note="GST C-terminal"
FT   BINDING         14
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         55
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         67..68
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   220 AA;  25225 MW;  2433BEA9D71D2AC8 CRC64;
     MESNNVVLLD FWPSSFGMRL RIALALKGIK YEAKEENLSD KSPLLLEMNP VHKKIPILIH
     NSKAICESLN ILEYIDEVWH DKCPLLPSDP YERSQARFWA DYIDKKIYST GRRVWSGKGE
     DQEEAKKEFI EILKTLEGEL GNKTYFGGDN LGFVDVALVP FTSWFYSYET CANFSIEAEC
     PKLVVWAKTC MESESVSKSL PHPHKIYGFV LELKHKLGLA