ID   GSTZ2_DIACA             Reviewed;         145 AA.
AC   Q03425;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Glutathione S-transferase 2;
DE            EC=2.5.1.18;
DE   AltName: Full=GST class-zeta;
DE   Flags: Fragment;
GN   Name=GST2;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. White Sim; TISSUE=Petal;
RX   PubMed=8499618; DOI=10.1007/bf00038994;
RA   Itzhaki H., Woodson W.R.;
RT   "Characterization of an ethylene-responsive glutathione S-transferase gene
RT   cluster in carnation.";
RL   Plant Mol. Biol. 22:43-58(1993).
CC   -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC       exogenous and endogenous hydrophobic electrophiles.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- DEVELOPMENTAL STAGE: Senescing flowers.
CC   -!- INDUCTION: Accumulates in response to the phytohormone ethylene.
CC   -!- SIMILARITY: Belongs to the GST superfamily. Zeta family. {ECO:0000305}.
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DR   EMBL; L05916; AAA51450.1; -; Genomic_DNA.
DR   PIR; S33629; S33629.
DR   AlphaFoldDB; Q03425; -.
DR   SMR; Q03425; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004364; F:glutathione transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009072; P:aromatic amino acid family metabolic process; IEA:InterPro.
DR   GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR   CDD; cd03042; GST_N_Zeta; 1.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR005955; GST_Zeta.
DR   InterPro; IPR034333; GST_Zeta_N.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   Pfam; PF13417; GST_N_3; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR01262; maiA; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   2: Evidence at transcript level;
KW   Transferase.
FT   CHAIN           1..>145
FT                   /note="Glutathione S-transferase 2"
FT                   /id="PRO_0000186032"
FT   DOMAIN          7..88
FT                   /note="GST N-terminal"
FT   DOMAIN          93..>145
FT                   /note="GST C-terminal"
FT   BINDING         17..22
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         60
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         72..73
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         112
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         116..118
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   NON_TER         145
SQ   SEQUENCE   145 AA;  16447 MW;  EEF49C6143D796B3 CRC64;
     MSSSETQKMQ LYSYSSSSCA WRVRIALHLK GLDFEYKAVD LLKGEHLTPE FLKLNPLGYV
     PALVHGDIVI ADSLAIIMYL EEKFPENPLL PRDLQKRALN YQAANIVASN IQPFQNLAVL
     NYIEEKLGSD EKLSWANHHI KKGFS