ID   GST_ASADI               Reviewed;          15 AA.
AC   P83246;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Glutathione S-transferase;
DE            EC=2.5.1.18;
DE   AltName: Full=GST class-sigma;
DE   AltName: Full=adGST;
DE   Flags: Fragment;
OS   Asaphis dichotoma.
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Heteroconchia; Euheterodonta; Imparidentia; Neoheterodontei;
OC   Cardiida; Tellinoidea; Psammobiidae; Asaphis.
OX   NCBI_TaxID=184428 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, AND CIRCULAR
RP   DICHROISM ANALYSIS.
RC   TISSUE=Gut, and Liver;
RX   PubMed=12139969; DOI=10.1016/s0003-9861(02)00223-0;
RA   Yang H.-L., Nie L.-J., Zhu S.-G., Zhou X.-W.;
RT   "Purification and characterization of a novel glutathione S-transferase
RT   from Asaphis dichotoma.";
RL   Arch. Biochem. Biophys. 403:202-208(2002).
CC   -!- FUNCTION: Has a strong specific activity toward 1-chloro-2,4-
CC       dinitrobenzene and ethacrynic acid. {ECO:0000269|PubMed:12139969}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC         Evidence={ECO:0000269|PubMed:12139969};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.68 mM for 1-chloro-2,4-dinitrobenzene
CC         {ECO:0000269|PubMed:12139969};
CC         KM=0.106 mM for glutathione {ECO:0000269|PubMed:12139969};
CC         Vmax=0.1446 mmol/min/mg enzyme toward CDNB
CC         {ECO:0000269|PubMed:12139969};
CC         Vmax=0.033 mmol/min/mg enzyme toward GSH
CC         {ECO:0000269|PubMed:12139969};
CC       pH dependence:
CC         Optimum pH is 8.5 with 1-chloro-2,4-dinitrobenzene as substrate.
CC         {ECO:0000269|PubMed:12139969};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12139969}.
CC   -!- MASS SPECTROMETRY: Mass=23138; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12139969};
CC   -!- MISCELLANEOUS: In A.dichotoma there are at least two isozymes of
CC       glutathione S-transferase. {ECO:0000269|PubMed:12139969}.
CC   -!- SIMILARITY: Belongs to the GST superfamily. Sigma family.
CC       {ECO:0000250|UniProtKB:P20137}.
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DR   GO; GO:0004364; F:glutathione transferase activity; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0006749; P:glutathione metabolic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Transferase.
FT   CHAIN           1..>15
FT                   /note="Glutathione S-transferase"
FT                   /id="PRO_0000185923"
FT   BINDING         7
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250|UniProtKB:O60760"
FT   NON_TER         15
FT                   /evidence="ECO:0000303|PubMed:12139969"
SQ   SEQUENCE   15 AA;  1767 MW;  CB3E4BF92D3CB0B9 CRC64;
     PSYKLHYFDL RAAGE