GSX2_MOUSE
ID GSX2_MOUSE Reviewed; 305 AA.
AC P31316;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=GS homeobox 2;
DE AltName: Full=Genetic-screened homeobox 2;
DE AltName: Full=Homeobox protein GSH-2;
GN Name=Gsx2; Synonyms=Gsh-2, Gsh2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=NIH Swiss;
RX PubMed=7619729; DOI=10.1016/0925-4773(94)00334-j;
RA Hsieh-Li H.M., Witte D.P., Szucsik J.C., Weinstein M., Li H., Potter S.S.;
RT "Gsh-2, a murine homeobox gene expressed in the developing brain.";
RL Mech. Dev. 50:177-186(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 203-262.
RX PubMed=1683707; DOI=10.1073/pnas.88.23.10706;
RA Singh G., Kaur S., Stock J.L., Jenkins N.A., Gilbert D.J., Copeland N.G.,
RA Potter S.S.;
RT "Identification of 10 murine homeobox genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:10706-10710(1991).
RN [3]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=19709628; DOI=10.1016/j.neuron.2009.07.015;
RA Waclaw R.R., Wang B., Pei Z., Ehrman L.A., Campbell K.;
RT "Distinct temporal requirements for the homeobox gene Gsx2 in specifying
RT striatal and olfactory bulb neuronal fates.";
RL Neuron 63:451-465(2009).
CC -!- FUNCTION: Transcription factor that binds 5'-CNAATTAG-3' DNA sequence
CC and regulates the expression of numerous genes including genes
CC important for brain development (PubMed:7619729). During telencephalic
CC development, causes ventralization of pallial progenitors and,
CC depending on the developmental stage, specifies different neuronal
CC fates. At early stages, necessary and sufficient to correctly specify
CC the ventral lateral ganglionic eminence (LGE) and its major
CC derivatives, the striatal projection neurons. At later stages, may
CC specify LGE progenitors toward dorsal LGE fates, including olfactory
CC bulb interneurons (PubMed:19709628). {ECO:0000269|PubMed:19709628,
CC ECO:0000269|PubMed:7619729}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: At 10.0 dpc, expressed in a band of primitive
CC neuroepithelial cells in the neural tube, mesencephalon and
CC telencephalon. At 11.5-13.5 dpc, expression is symmetrical, but tightly
CC limited in areas of the forebrain, midbrain and hindbrain
CC (PubMed:7619729). At 11 dpc, in the developing telencephalon, expressed
CC at high levels in cells throughout the presumptive lateral ganglionic
CC eminence (LGE), with an apparent ventral-to-dorsal gradient in
CC expressing cell numbers. Positive cells are also scattered somewhat
CC uniformly throughout the adjacent medial ganglionic eminence. At 12.5
CC dpc onward, exhibits a clear graded pattern of expression, with low
CC levels found in cells located ventrally and the highest levels confined
CC to those in the most dorsal portion of the LGE (at protein level)
CC (PubMed:19709628). Expression decreases from 14.5 dpc on and becomes
CC undetectable at 16.5 dpc (PubMed:7619729).
CC {ECO:0000269|PubMed:19709628, ECO:0000269|PubMed:7619729}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR EMBL; S79041; AAB34947.1; -; mRNA.
DR CCDS; CCDS19350.1; -.
DR PIR; B37290; B37290.
DR RefSeq; NP_573555.1; NM_133256.2.
DR AlphaFoldDB; P31316; -.
DR SMR; P31316; -.
DR BioGRID; 200086; 1.
DR STRING; 10090.ENSMUSP00000036625; -.
DR PaxDb; P31316; -.
DR PRIDE; P31316; -.
DR Antibodypedia; 12271; 117 antibodies from 23 providers.
DR DNASU; 14843; -.
DR Ensembl; ENSMUST00000040477; ENSMUSP00000036625; ENSMUSG00000035946.
DR GeneID; 14843; -.
DR KEGG; mmu:14843; -.
DR UCSC; uc008xtx.1; mouse.
DR CTD; 170825; -.
DR MGI; MGI:95843; Gsx2.
DR VEuPathDB; HostDB:ENSMUSG00000035946; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000156043; -.
DR HOGENOM; CLU_077153_0_0_1; -.
DR InParanoid; P31316; -.
DR OMA; HAPVCTP; -.
DR OrthoDB; 1341872at2759; -.
DR PhylomeDB; P31316; -.
DR TreeFam; TF315938; -.
DR BioGRID-ORCS; 14843; 2 hits in 72 CRISPR screens.
DR PRO; PR:P31316; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P31316; protein.
DR Bgee; ENSMUSG00000035946; Expressed in ureteric bud trunk and 39 other tissues.
DR ExpressionAtlas; P31316; baseline and differential.
DR Genevisible; P31316; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007420; P:brain development; IGI:MGI.
DR GO; GO:0007417; P:central nervous system development; IMP:MGI.
DR GO; GO:0021798; P:forebrain dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0048853; P:forebrain morphogenesis; IMP:MGI.
DR GO; GO:0097154; P:GABAergic neuron differentiation; IMP:MGI.
DR GO; GO:0048699; P:generation of neurons; IMP:MGI.
DR GO; GO:0021575; P:hindbrain morphogenesis; IMP:MGI.
DR GO; GO:0048663; P:neuron fate commitment; IGI:MGI.
DR GO; GO:0048665; P:neuron fate specification; IMP:MGI.
DR GO; GO:0007219; P:Notch signaling pathway; IMP:MGI.
DR GO; GO:0021772; P:olfactory bulb development; IMP:MGI.
DR GO; GO:0021889; P:olfactory bulb interneuron differentiation; IMP:MGI.
DR GO; GO:0007389; P:pattern specification process; IMP:MGI.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:MGI.
DR GO; GO:0048714; P:positive regulation of oligodendrocyte differentiation; IMP:MGI.
DR GO; GO:0030334; P:regulation of cell migration; IMP:MGI.
DR GO; GO:0002087; P:regulation of respiratory gaseous exchange by nervous system process; IMP:MGI.
DR GO; GO:0021527; P:spinal cord association neuron differentiation; IGI:MGI.
DR GO; GO:0021544; P:subpallium development; IGI:MGI.
DR GO; GO:0060163; P:subpallium neuron fate commitment; IMP:MGI.
DR GO; GO:0021978; P:telencephalon regionalization; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR042191; GSH2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR PANTHER; PTHR47421; PTHR47421; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..305
FT /note="GS homeobox 2"
FT /id="PRO_0000048897"
FT DNA_BIND 203..262
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 115..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..141
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 281..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 305 AA; 32167 MW; 51E7F2DB76E32608 CRC64;
MSRSFYVDSL IIKDSSRPAP SLPESHPGPD FFIPLGMPSP LVMSVSGPGC PSRKSGAFCV
CPLCVTSHLH SSRPPAGAGG GATGTAGAAV AGGGVAGGTG ALPLLKSQFS PAPGDAQFCP
RVSHAHHHHH PPQHHHHHHQ PQQPGSAAAA AAAAAAAAAA AAALGHPQHH APVCAATTYN
MSDPRRFHCL SMGGSDTSQV PNGKRMRTAF TSTQLLELER EFSSNMYLSR LRRIEIATYL
NLSEKQVKIW FQNRRVKHKK EGKGASRNNH TSCKCVGSQA HYARSEDEDS LSPASANEDK
EISPL
