GSXL8_ARATH
ID GSXL8_ARATH Reviewed; 461 AA.
AC Q9FLK4;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Flavin-containing monooxygenase FMO GS-OX-like 8 {ECO:0000303|PubMed:17461789};
DE EC=1.8.-.- {ECO:0000305};
DE AltName: Full=Flavin-monooxygenase glucosinolate S-oxygenase-like 8 {ECO:0000303|PubMed:17461789};
GN OrderedLocusNames=At5g61290 {ECO:0000312|Araport:AT5G61290};
GN ORFNames=MFB13.9 {ECO:0000312|EMBL:BAB08484.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=17461789; DOI=10.1111/j.1365-313x.2007.03101.x;
RA Hansen B.G., Kliebenstein D.J., Halkier B.A.;
RT "Identification of a flavin-monooxygenase as the S-oxygenating enzyme in
RT aliphatic glucosinolate biosynthesis in Arabidopsis.";
RL Plant J. 50:902-910(2007).
RN [6]
RP INTERACTION WITH EER5.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
CC -!- FUNCTION: Catalyzes the conversion of methylthioalkyl glucosinolates of
CC any chain length into methylsulfinylalkyl glucosinolates.
CC {ECO:0000250|UniProtKB:Q9SS04}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC -!- SUBUNIT: Interacts with EER5. {ECO:0000269|PubMed:19843313}.
CC -!- SIMILARITY: Belongs to the FMO family. {ECO:0000305}.
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DR EMBL; AB010073; BAB08484.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97448.1; -; Genomic_DNA.
DR EMBL; AK117661; BAC42314.1; -; mRNA.
DR EMBL; BT006095; AAP04080.1; -; mRNA.
DR RefSeq; NP_200937.1; NM_125522.4.
DR AlphaFoldDB; Q9FLK4; -.
DR SMR; Q9FLK4; -.
DR BioGRID; 21494; 1.
DR IntAct; Q9FLK4; 1.
DR STRING; 3702.AT5G61290.1; -.
DR PaxDb; Q9FLK4; -.
DR PRIDE; Q9FLK4; -.
DR ProteomicsDB; 247229; -.
DR EnsemblPlants; AT5G61290.1; AT5G61290.1; AT5G61290.
DR GeneID; 836250; -.
DR Gramene; AT5G61290.1; AT5G61290.1; AT5G61290.
DR KEGG; ath:AT5G61290; -.
DR Araport; AT5G61290; -.
DR TAIR; locus:2163193; AT5G61290.
DR eggNOG; KOG1399; Eukaryota.
DR HOGENOM; CLU_006909_3_3_1; -.
DR InParanoid; Q9FLK4; -.
DR OMA; ASCKKVC; -.
DR OrthoDB; 405736at2759; -.
DR PhylomeDB; Q9FLK4; -.
DR BioCyc; ARA:AT5G61290-MON; -.
DR PRO; PR:Q9FLK4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLK4; baseline and differential.
DR Genevisible; Q9FLK4; AT.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000960; Flavin_mOase.
DR InterPro; IPR020946; Flavin_mOase-like.
DR Pfam; PF00743; FMO-like; 2.
DR PIRSF; PIRSF000332; FMO; 1.
DR PRINTS; PR00370; FMOXYGENASE.
DR SUPFAM; SSF51905; SSF51905; 2.
PE 1: Evidence at protein level;
KW FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..461
FT /note="Flavin-containing monooxygenase FMO GS-OX-like 8"
FT /id="PRO_0000401963"
FT BINDING 20..25
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 220..225
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000255"
SQ SEQUENCE 461 AA; 52407 MW; EFC61B6D7AFB49BC CRC64;
MVLVITEPIK SQSKTVCVIG AGPSGLVSAR ELKKEGHKVV VMEQNHDVGG QWLYQPNVDE
EDTLGKTKTL KVHSSVYSSL RLASPREVMG FSDFPFIAKE GRDSRRFPGH EELLLYLKDF
CQVFGLREMI RFNVRVEFVG MVNEDDDDDD DVKKWMVKSV KKSGEVMEEV FDAVVVASGH
YSYPRLPTIK GMDLWKRKQL HSHIYRVPEP FCDEVVVVVG CSMSGQDISI ELVEVAKEVH
LSTKSLDIPP GLSKVIEKHQ NLHLHPQIES LEEDGRVIFE DGSCIVADTI LYCTGYEYKF
PFLESKGRVE IDDNRVGPLF EHTFSPSLSP FLSFVGIPRK LIGFPFFESQ AKWIAKLLSG
KTSLPSSDQM MQSISDFYLA READGIPKRN THDIADFNYS DKYADYIGFP HLEEWRKVLC
LSAILNSIEN LETYRDSWDD DDLLQETLQD PYFTQLSIPS V
