GT101_ORYSJ
ID GT101_ORYSJ Reviewed; 417 AA.
AC Q33AH8; Q33AI0; Q8GSQ3; Q8LNA1; Q8SB14;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Probable glucuronosyltransferase GUT1;
DE EC=2.4.-.-;
DE AltName: Full=Glucuronoxylan glucuronosyltransferase 1;
DE Short=OsGUT1;
GN Name=GUT1; OrderedLocusNames=Os10g0180000, LOC_Os10g10080;
GN ORFNames=OSJNBa0047G15.26, OSJNBa0095J15.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12791992; DOI=10.1126/science.1083523;
RA Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT "In-depth view of structure, activity, and evolution of rice chromosome
RT 10.";
RL Science 300:1566-1569(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 18-417.
RC STRAIN=cv. Nipponbare;
RX PubMed=12451175; DOI=10.1073/pnas.252530499;
RA Iwai H., Masaoka N., Ishii T., Satoh S.;
RT "A pectin glucuronyltransferase gene is essential for intercellular
RT attachment in the plant meristem.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16319-16324(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 77-417.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Involved in the synthesis of glucuronoxylan hemicellulose in
CC secondary cell walls. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q33AH8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q33AH8-2; Sequence=VSP_040958;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL76189.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAM74308.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABB46945.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=ABB46946.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC083944; AAM74308.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC092173; AAL76189.1; ALT_INIT; Genomic_DNA.
DR EMBL; DP000086; ABB46945.2; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000086; ABB46946.2; ALT_INIT; Genomic_DNA.
DR EMBL; DP000086; ABB46947.2; -; Genomic_DNA.
DR EMBL; AP008216; BAF26162.1; -; Genomic_DNA.
DR EMBL; AP014966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB080694; BAC20930.1; -; mRNA.
DR EMBL; AK109393; BAG98714.1; -; mRNA.
DR RefSeq; XP_015614619.1; XM_015759133.1. [Q33AH8-1]
DR RefSeq; XP_015614620.1; XM_015759134.1.
DR AlphaFoldDB; Q33AH8; -.
DR STRING; 4530.OS10T0180000-01; -.
DR CAZy; GT47; Glycosyltransferase Family 47.
DR PaxDb; Q33AH8; -.
DR PRIDE; Q33AH8; -.
DR EnsemblPlants; Os10t0180000-01; Os10t0180000-01; Os10g0180000. [Q33AH8-1]
DR GeneID; 4348204; -.
DR Gramene; Os10t0180000-01; Os10t0180000-01; Os10g0180000. [Q33AH8-1]
DR KEGG; osa:4348204; -.
DR eggNOG; KOG1021; Eukaryota.
DR HOGENOM; CLU_039682_1_0_1; -.
DR InParanoid; Q33AH8; -.
DR OMA; RSTHMFS; -.
DR Proteomes; UP000000763; Chromosome 10.
DR Proteomes; UP000059680; Chromosome 10.
DR ExpressionAtlas; Q33AH8; baseline and differential.
DR Genevisible; Q33AH8; OS.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010417; P:glucuronoxylan biosynthetic process; IBA:GO_Central.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IBA:GO_Central.
DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR InterPro; IPR004263; Exostosin.
DR InterPro; IPR040911; Exostosin_GT47.
DR PANTHER; PTHR11062; PTHR11062; 1.
DR Pfam; PF03016; Exostosin; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall biogenesis/degradation; Glycoprotein;
KW Glycosyltransferase; Golgi apparatus; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..417
FT /note="Probable glucuronosyltransferase GUT1"
FT /id="PRO_0000407573"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..417
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 405
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 44
FT /note="K -> KE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040958"
FT CONFLICT 314..317
FT /note="VLPF -> DLPL (in Ref. 1; AAL76189 and 5; BAC20930)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 417 AA; 47300 MW; 85B0F99975F97EF5 CRC64;
MGTRRRSARA RARPPLAMPL AVLLLFACSS GVAAAAAQGI ERIKDDPVGK LKVYVYELPP
KYNKNIVAKD SRCLSHMFAT EIFMHRFLLS SAIRTSNPDE ADWFYTPVYT TCDLTPWGHP
LTTKSPRMMR SAIKFISKYW PYWNRTEGAD HFFVVPHDFA ACFYFQEAKA IERGILPVLR
RATLVQTFGQ KNHACLKDGS ITVPPYTPAH KIRAHLVPPE TPRSIFVYFR GLFYDTSNDP
EGGYYARGAR ASVWENFKNN PMFDISTDHP QTYYEDMQRA VFCLCPLGWA PWSPRLVEAV
VFGCIPVIIA DDIVLPFSDA IPWEEIAVFV AEDDVPQLDT ILTSIPTEVI LRKQAMLAEP
SMKQTMLFPQ PAEPGDGFHQ VMNALARKLP HGRDVFLKPG QKVLNWTEGT REDLKPW
