GT12_ARATH
ID GT12_ARATH Reviewed; 509 AA.
AC O48842;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Probable xyloglucan galactosyltransferase GT12 {ECO:0000305};
DE EC=2.4.1.- {ECO:0000305};
DE AltName: Full=Glycosyltransferase 12 {ECO:0000303|PubMed:15020758};
DE Short=AtGT12 {ECO:0000303|PubMed:15020758};
GN Name=GT12 {ECO:0000303|PubMed:15020758};
GN OrderedLocusNames=At2g32750 {ECO:0000312|Araport:AT2G32750};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15020758; DOI=10.1104/pp.103.036285;
RA Li X., Cordero I., Caplan J., Moelhoej M., Reiter W.D.;
RT "Molecular analysis of 10 coding regions from Arabidopsis that are
RT homologous to the MUR3 xyloglucan galactosyltransferase.";
RL Plant Physiol. 134:940-950(2004).
CC -!- FUNCTION: Functions in xyloglucan synthesis by adding side chains to
CC the xylosylated glucan backbone. Involved in the galactosylation of
CC hemicellulose xyloglucan. {ECO:0000250|UniProtKB:F4K6F1}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q7XJ98}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:Q7XJ98}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen grains.
CC {ECO:0000269|PubMed:15020758}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC {ECO:0000305}.
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DR EMBL; KJ138877; AHL38817.1; -; mRNA.
DR EMBL; AC003974; AAC04488.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08732.1; -; Genomic_DNA.
DR PIR; T00793; T00793.
DR RefSeq; NP_180834.1; NM_128834.2.
DR AlphaFoldDB; O48842; -.
DR IntAct; O48842; 1.
DR STRING; 3702.AT2G32750.1; -.
DR CAZy; GT47; Glycosyltransferase Family 47.
DR PaxDb; O48842; -.
DR PRIDE; O48842; -.
DR EnsemblPlants; AT2G32750.1; AT2G32750.1; AT2G32750.
DR GeneID; 817835; -.
DR Gramene; AT2G32750.1; AT2G32750.1; AT2G32750.
DR KEGG; ath:AT2G32750; -.
DR Araport; AT2G32750; -.
DR TAIR; locus:2046372; AT2G32750.
DR eggNOG; KOG1021; Eukaryota.
DR HOGENOM; CLU_012659_4_1_1; -.
DR InParanoid; O48842; -.
DR PhylomeDB; O48842; -.
DR PRO; PR:O48842; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48842; baseline and differential.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR InterPro; IPR004263; Exostosin.
DR InterPro; IPR040911; Exostosin_GT47.
DR PANTHER; PTHR11062; PTHR11062; 1.
DR Pfam; PF03016; Exostosin; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..509
FT /note="Probable xyloglucan galactosyltransferase GT12"
FT /id="PRO_0000435994"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 4..24
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..509
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 65
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 257
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 416
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 509 AA; 59135 MW; D1C3AE95A74FDC68 CRC64;
MMKPVPKLWV VISSAFVFCL LVLFQINKSD LIEANLQITH QVNNFLISFV ASSNNQILNH
TKHANESDGI RAKQLEEEET DTCAGRYIYM YNLPSTFNDD IIKECRPLIK WFDMCPFMVN
SGLGPQILVS DKTTARVLTV KTGSWYSTNQ FLLSVIFRER MKHYECLTNN SSLASAIYVP
YYAGFDVSRH LWGYNVTVRD ELAIKLAQWL RERPEWGKMY GRDHFFVTGR IGWDFRRFHD
EDSDWGSKLM LLPEFSNLTM LGIETTAWAN EFAIPYPTYF HPKSLTEIWR WQKKVKSVKR
KYLFSFVGGP RPKLDGSIRG EIIKQCLASH GKCNFLNCFV NDCDNPVKIM KVFENSVFCL
QPSGDSYTRR SIFDSILAGC IPVFFSPGSG YNQYIWYFPK DYTKYSVYIP ENEMRNGTVS
LKNILGMIAK ERILRMRKEV VKIIPKIIYN KPGFGPEKIE DAFDIAVDRM LERVAMVKRM
MEEGKDVQSQ YYSQTKDLKK LEIIHEKTA
