GT13_ARATH
ID GT13_ARATH Reviewed; 468 AA.
AC O48843;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Probable xyloglucan galactosyltransferase GT13 {ECO:0000305};
DE EC=2.4.1.- {ECO:0000305};
DE AltName: Full=Glycosyltransferase 13 {ECO:0000303|PubMed:15020758};
DE Short=AtGT13 {ECO:0000303|PubMed:15020758};
GN Name=GT13 {ECO:0000303|PubMed:15020758};
GN OrderedLocusNames=At2g32740 {ECO:0000312|Araport:AT2G32740};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15020758; DOI=10.1104/pp.103.036285;
RA Li X., Cordero I., Caplan J., Moelhoej M., Reiter W.D.;
RT "Molecular analysis of 10 coding regions from Arabidopsis that are
RT homologous to the MUR3 xyloglucan galactosyltransferase.";
RL Plant Physiol. 134:940-950(2004).
CC -!- FUNCTION: Functions in xyloglucan synthesis by adding side chains to
CC the xylosylated glucan backbone. Involved in the galactosylation of
CC hemicellulose xyloglucan. {ECO:0000250|UniProtKB:F4K6F1}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q7XJ98}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:Q7XJ98}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, hypocotyls, cotyledons, leaves,
CC stems, petals and carpels. {ECO:0000269|PubMed:15020758}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC {ECO:0000305}.
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DR EMBL; KJ138878; AHL38818.1; -; mRNA.
DR EMBL; AC003974; AAC04489.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08731.1; -; Genomic_DNA.
DR PIR; T00794; T00794.
DR RefSeq; NP_180833.1; NM_128833.2.
DR AlphaFoldDB; O48843; -.
DR STRING; 3702.AT2G32740.1; -.
DR CAZy; GT47; Glycosyltransferase Family 47.
DR PaxDb; O48843; -.
DR PRIDE; O48843; -.
DR EnsemblPlants; AT2G32740.1; AT2G32740.1; AT2G32740.
DR GeneID; 817834; -.
DR Gramene; AT2G32740.1; AT2G32740.1; AT2G32740.
DR KEGG; ath:AT2G32740; -.
DR Araport; AT2G32740; -.
DR TAIR; locus:2046387; AT2G32740.
DR eggNOG; KOG1021; Eukaryota.
DR HOGENOM; CLU_012659_4_1_1; -.
DR InParanoid; O48843; -.
DR OMA; PISSDAH; -.
DR OrthoDB; 789556at2759; -.
DR PhylomeDB; O48843; -.
DR PRO; PR:O48843; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48843; baseline and differential.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR InterPro; IPR004263; Exostosin.
DR InterPro; IPR040911; Exostosin_GT47.
DR PANTHER; PTHR11062; PTHR11062; 1.
DR Pfam; PF03016; Exostosin; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..468
FT /note="Probable xyloglucan galactosyltransferase GT13"
FT /id="PRO_0000435995"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 19..35
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..468
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT CARBOHYD 53
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 240
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 468 AA; 54837 MW; D817639DA30431F2 CRC64;
MDKFNPKKEK TVKKRALKVL TEISPTPLFS MLFLLHISQI ATYLSLSDKE TLNITVKTKQ
GGTDTCAGRY VYMHNLPSRF NEDLIKSCEA YIELRNKCKY LINSGFGPRI LEEDHNHTTR
VLTIETGSWY YTNQFMLEVI FREKMRHYEC LTNDSSLSSV VFVPFYAGFD VRRFWGYNVK
LRDELGEDLA QWLRERPEWR KMYGRDHFFV TGRVGRDFRR VTDQDSDWGN KLMRLPEFEN
ITMLSIETNS RSNEFAVPYP TYFHPKSRTE VKRWQRQVTM MQRRYLFSFV GANRPKMEES
IRGEIIRQCL ASQGRCKFLD CDTSSKDCSD PVKVVEVFQD SVFCLQPPGD TPTRRSTFDS
ILAGCIPVFF SVDSVYNQYK WYFPKDRTKY SVYIAEEGVK KGKVSIEKLL ANVSEEKISR
MRNEVEKIIP KIIYTKPGEV GPEKIEDAFE IAVARVLERV SLFKMTRI
