GT15_ARATH
ID GT15_ARATH Reviewed; 479 AA.
AC Q5XF04; Q9SL00;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Probable xyloglucan galactosyltransferase GT15 {ECO:0000305};
DE EC=2.4.1.- {ECO:0000305};
DE AltName: Full=Glycosyltransferase 15 {ECO:0000303|PubMed:15020758};
DE Short=AtGT15 {ECO:0000303|PubMed:15020758};
GN Name=GT15 {ECO:0000303|PubMed:15020758};
GN OrderedLocusNames=At2g31990 {ECO:0000312|Araport:AT2G31990};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15020758; DOI=10.1104/pp.103.036285;
RA Li X., Cordero I., Caplan J., Moelhoej M., Reiter W.D.;
RT "Molecular analysis of 10 coding regions from Arabidopsis that are
RT homologous to the MUR3 xyloglucan galactosyltransferase.";
RL Plant Physiol. 134:940-950(2004).
CC -!- FUNCTION: Functions in xyloglucan synthesis by adding side chains to
CC the xylosylated glucan backbone. Involved in the galactosylation of
CC hemicellulose xyloglucan. {ECO:0000250|UniProtKB:F4K6F1}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q7XJ98}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:Q7XJ98}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, hypocotyls, cotyledons, leaves,
CC stems and sepals. {ECO:0000269|PubMed:15020758}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD15405.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; KJ138883; AHL38823.1; -; mRNA.
DR EMBL; AC006223; AAD15405.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08617.1; -; Genomic_DNA.
DR EMBL; BT015812; AAU94375.1; -; mRNA.
DR EMBL; BT021113; AAX12883.1; -; mRNA.
DR PIR; F84727; F84727.
DR RefSeq; NP_001324318.1; NM_001336365.1.
DR RefSeq; NP_180759.2; NM_128759.3.
DR AlphaFoldDB; Q5XF04; -.
DR STRING; 3702.AT2G31990.1; -.
DR CAZy; GT47; Glycosyltransferase Family 47.
DR PaxDb; Q5XF04; -.
DR PRIDE; Q5XF04; -.
DR ProteomicsDB; 247259; -.
DR EnsemblPlants; AT2G31990.1; AT2G31990.1; AT2G31990.
DR GeneID; 817758; -.
DR Gramene; AT2G31990.1; AT2G31990.1; AT2G31990.
DR KEGG; ath:AT2G31990; -.
DR Araport; AT2G31990; -.
DR TAIR; locus:2045527; AT2G31990.
DR eggNOG; KOG1021; Eukaryota.
DR HOGENOM; CLU_012659_4_1_1; -.
DR InParanoid; Q5XF04; -.
DR PRO; PR:Q5XF04; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q5XF04; baseline and differential.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR InterPro; IPR004263; Exostosin.
DR InterPro; IPR040911; Exostosin_GT47.
DR PANTHER; PTHR11062; PTHR11062; 1.
DR Pfam; PF03016; Exostosin; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..479
FT /note="Probable xyloglucan galactosyltransferase GT15"
FT /id="PRO_0000435997"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 21..40
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..479
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT CARBOHYD 155
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 242
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 285
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 479 AA; 54788 MW; 97C5A646D8881FD2 CRC64;
MKNNNSSSVS IENHPWKKKP TTLLLFLSLL SISLLLLRLS QDKIILITTT KTTATTGTDH
QRSHKSSEDD TCLGRYIYIH NLPSRFNLEI IKDCKSITRP KDKISMCKYL DNSGFGPLIG
GKSSDYSPSW YATNQFMLEV IFHEKMKSYE CLTRNSSLAS AIYVPYYAGL DFRRHLRRRN
VAARDAAGKE LVKWLKKQPQ WKDMSGKNHF LVTGRISRDF RRNSGSRSAW GTNFMLLSES
LNLTFLSIER SLTSHNEFAI PYPTYFHPTS TPEILQWQEK IRLTNRTVLF SFAGAQRPSR
NQNGVVRTEV IKQCKSSSKT CRFLDCDVNA NSCDDPISLM KLFESSTFCL QPPGDSLTRK
SVFDSILAGC IPVFFNQGSA YKQYLWHIPK NSSKYSVYIT VKELRTGGKN KIEEILRGIP
NERVVGMREN VIRLIPKIVY AKPNRNKPDG EILEDSFDVA VKGVLERIEG IRRNEFKTD
