GT2D1_XENLA
ID GT2D1_XENLA Reviewed; 993 AA.
AC B7ZQJ9; Q8JJ22;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=General transcription factor II-I repeat domain-containing protein 1 {ECO:0000250|UniProtKB:Q9UHL9};
DE AltName: Full=Binding factor for early enhancer {ECO:0000303|PubMed:16055724};
DE Short=BEN {ECO:0000303|PubMed:16055724};
DE Short=XBEN {ECO:0000303|PubMed:16055724};
DE AltName: Full=GTF2I repeat domain-containing protein 1;
DE AltName: Full=Williams-Beuren syndrome critical region 11-like protein {ECO:0000312|EMBL:AAM28813.1};
DE Short=XWBSCR11 {ECO:0000250|UniProtKB:Q9UHL9, ECO:0000303|PubMed:11937490};
GN Name=gtf2ird1; Synonyms=wbscr11 {ECO:0000312|EMBL:AAI69835.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAM28813.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH FOXH1; SMAD2 AND
RP SMAD3, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Oocyte {ECO:0000269|PubMed:11937490};
RX PubMed=11937490; DOI=10.1101/gad.963802;
RA Ring C., Ogata S., Meek L., Song J., Ohta T., Miyazono K., Cho K.W.;
RT "The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-
RT containing transcription factor in activin/nodal signaling.";
RL Genes Dev. 16:820-835(2002).
RN [2] {ECO:0000312|EMBL:AAI69835.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAI69835.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=16055724; DOI=10.1128/mcb.25.16.7144-7157.2005;
RA Ku M., Sokol S.Y., Wu J., Tussie-Luna M.I., Roy A.L., Hata A.;
RT "Positive and negative regulation of the transforming growth factor
RT beta/activin target gene goosecoid by the TFII-I family of transcription
RT factors.";
RL Mol. Cell. Biol. 25:7144-7157(2005).
RN [4] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH POU5F1.1.
RX PubMed=18922797; DOI=10.1074/jbc.m803532200;
RA Cao Y., Siegel D., Oswald F., Knochel W.;
RT "Oct25 represses transcription of nodal/activin target genes by interaction
RT with signal transducers during Xenopus gastrulation.";
RL J. Biol. Chem. 283:34168-34177(2008).
CC -!- FUNCTION: Transcription factor that activates a subset of organizer-
CC specific genes. Binds to the distal element (DE) of the gsc promoter to
CC regulate its expression. In the presence of pou5f1.1/oct-25, forms a
CC repression complex on the promoter of the gsc and mix2 genes to inhibit
CC their transcription. {ECO:0000269|PubMed:11937490,
CC ECO:0000269|PubMed:16055724, ECO:0000269|PubMed:18922797}.
CC -!- SUBUNIT: Interacts (via repeats 4-5) with foxh1/fast1 (via Fork-head
CC domain). Interacts with smad2 and smad3 (via MH1 domain) in a ligand
CC (activin)-dependent manner. Interacts with pou5f1.1/oct-25 to form a
CC repression complex on the promoters of the gsc and mix2 genes.
CC {ECO:0000269|PubMed:11937490, ECO:0000269|PubMed:18922797}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UHL9,
CC ECO:0000255|PROSITE-ProRule:PRU00484}.
CC -!- TISSUE SPECIFICITY: Uniformly expressed in the embryo in pre- and early
CC gastrula stages. Enriched in the head region of early neurula through
CC tailbud stages. {ECO:0000269|PubMed:11937490}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically at
CC relatively consistent levels throughout development.
CC {ECO:0000269|PubMed:11937490}.
CC -!- SIMILARITY: Belongs to the TFII-I family. {ECO:0000255|PROSITE-
CC ProRule:PRU00484}.
CC -!- CAUTION: There is conflicting evidence as to whether gtf2ird1 acts as a
CC transcriptional activator or repressor when directly bound to the
CC distal element of the gsc promoter. PubMed:11937490 shows that gtf2ird1
CC acts cooperatively with smads and foxh1/fast1 to activate
CC activin/nodal-mediated gsc transcription. However, PubMed:16055724
CC report that in the absence of TGF-beta, gtf2ird1 represses gsc
CC transcription. Upon TGF-beta stimulation, smad2 is translocated to the
CC nucleus as a complex with smad4, interacts with TFII-I, and binds to
CC the distal element to dislodge gtf2ird1 and up-regulate gsc gene
CC transcription. {ECO:0000269|PubMed:11937490,
CC ECO:0000269|PubMed:16055724, ECO:0000305}.
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DR EMBL; AF482757; AAM28813.1; -; mRNA.
DR EMBL; BC169835; AAI69835.1; -; mRNA.
DR RefSeq; NP_001082250.1; NM_001088781.1.
DR AlphaFoldDB; B7ZQJ9; -.
DR SMR; B7ZQJ9; -.
DR PRIDE; B7ZQJ9; -.
DR GeneID; 398320; -.
DR KEGG; xla:398320; -.
DR CTD; 398320; -.
DR Xenbase; XB-GENE-5857419; gtf2ird1.S.
DR OrthoDB; 115381at2759; -.
DR Proteomes; UP000186698; Chromosome 2S.
DR Bgee; 398320; Expressed in egg cell and 17 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IPI:UniProtKB.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0046332; F:SMAD binding; IPI:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0032925; P:regulation of activin receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR Gene3D; 3.90.1460.10; -; 5.
DR InterPro; IPR004212; GTF2I.
DR InterPro; IPR036647; GTF2I-like_rpt_sf.
DR InterPro; IPR016659; TF_II-I.
DR Pfam; PF02946; GTF2I; 5.
DR PIRSF; PIRSF016441; TF_II-I; 1.
DR SUPFAM; SSF117773; SSF117773; 5.
DR PROSITE; PS51139; GTF2I; 5.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Repeat; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..993
FT /note="General transcription factor II-I repeat domain-
FT containing protein 1"
FT /id="PRO_0000390740"
FT REPEAT 117..211
FT /note="GTF2I-like 1"
FT /evidence="ECO:0000255"
FT REPEAT 332..426
FT /note="GTF2I-like 2"
FT /evidence="ECO:0000255"
FT REPEAT 597..691
FT /note="GTF2I-like 3"
FT /evidence="ECO:0000255"
FT REPEAT 727..821
FT /note="GTF2I-like 4"
FT /evidence="ECO:0000255"
FT REPEAT 824..918
FT /note="GTF2I-like 5"
FT /evidence="ECO:0000255"
FT REGION 461..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..961
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 929..936
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:Q9UHL9"
FT COMPBIAS 939..961
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 513
FT /note="I -> L (in Ref. 1; AAM28813)"
FT /evidence="ECO:0000305"
FT CONFLICT 579
FT /note="K -> KR (in Ref. 1; AAM28813)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 993 AA; 110574 MW; C903FCF89748061E CRC64;
MALAGKQYEG SLNNSRPNLW PSSIPGGKNE IITSLVTALD SMCTALSKLN TEVACIALHE
ESAFVVGTEK GRCFLNSRKE LQADFQRFCI GAHKKDQENE VKRRNRDGIQ NIQHVPLGPT
SDIYLLRKMV EEIFEVLYSE ALGKSNIVPV PYVKVMKEPG SVVVLGLPDG ISFRKPAEYD
LKSLMLILKH SHNIRFKLRI PTEESIREPK SCSELNSPPT SATKVIPETS QCHRLPIQEH
PSSASNFPYS VSQPNQISLE PKQEVHSNML GTNAVNQMLV QRPSAENNHD LSDCCGQQSP
VAGSSLRQNV LASKHLLFSI VHDKTDKWDS FLRETEDINI LRECVQILFN SRYAGALGLD
HMVPVPYRKI ACHPEAVEIN GFPDKIPFKR PCTYGVPKLK RILEERHNIH FTIKSMFDQR
IFDGTTFTKE TTKSDSSSLG EEACSENQKA AALDMLGFPT GSRSEKSSIS DECEPGTSSE
TTGVKLIKLE AEDPDIMQIA VPGTSSETSG VKIIKLESED PDLIQITVPG TSNETSGVKP
KLESEDPDLI QIAVPDRLAE CVKNHLAPED PSYVLDTGKV CDDRPCGVLR NENKHLDGIG
DIIRQLRKHV ENLFNRKYAK AIGASGPVKV PYAKFLMYPE DLFVLGLPEG VAFRRPNCFG
ITKLRRILEY SDGIQFVVKR PELISEGLED CVVGSPGTLG FNDKSNEVIL DETNTRPSFQ
ESFDARLSRI DIANTLREQV QVLFNKKYGE ALGIKYPVQV PYKRIKNNPG SVIIEGLPPG
IPFRKPCTFG SQNLERILAV ADKIRFTVTR PFQGLIPRPD DEDANRLGEK VILREQVKEL
FNEKYGKALG LDQPALIPYK LIRDNPDAVE VRGMPNDIPF RNPNSYDLHR LENILKAQDQ
IQMVVIKQLE PFPEICSEPP KIKNGNTGPK RKRKRVSEGN SISSASSNCS SSSSSSSNMD
PISSAHHVSL VQWPMYMLDY GGLNMQLPGP INY
