ID   GT43E_ORYSJ             Reviewed;         451 AA.
AC   Q6AT32; A0A023NDJ9;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Probable beta-1,4-xylosyltransferase GT43E {ECO:0000305};
DE            EC=2.4.2.- {ECO:0000305};
DE   AltName: Full=OsGT43E {ECO:0000303|PubMed:24525904};
DE   AltName: Full=Probable glucuronosyltransferase Os05g0559600 {ECO:0000305};
GN   Name=GT43E {ECO:0000303|PubMed:24525904};
GN   OrderedLocusNames=Os05g0559600 {ECO:0000312|EMBL:BAS95305.1},
GN   LOC_Os05g48600 {ECO:0000305};
GN   ORFNames=OJ1115_B06.1 {ECO:0000312|EMBL:AAU10637.1},
GN   OSJNBa0001A14.15 {ECO:0000312|EMBL:AAT85103.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24525904; DOI=10.4161/psb.27809;
RA   Lee C., Teng Q., Zhong R., Yuan Y., Ye Z.H.;
RT   "Functional roles of rice glycosyltransferase family GT43 in xylan
RT   biosynthesis.";
RL   Plant Signal. Behav. 9:E27809-E27809(2014).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Probable beta-1,4-xylosyltransferase involved in xylan
CC       biosynthesis in cell walls. {ECO:0000269|PubMed:24525904}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:24525904}; Single-pass type II membrane protein
CC       {ECO:0000305|PubMed:24525904}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6AT32-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6AT32-2; Sequence=VSP_040957;
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 43 family.
CC       {ECO:0000305}.
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DR   EMBL; KJ206902; AHW98785.1; -; mRNA.
DR   EMBL; AC113333; AAU10637.1; -; Genomic_DNA.
DR   EMBL; AC144735; AAT85103.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF18215.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS95305.1; -; Genomic_DNA.
DR   EMBL; AK068237; BAG90818.1; -; mRNA.
DR   RefSeq; XP_015640357.1; XM_015784871.1. [Q6AT32-1]
DR   RefSeq; XP_015640358.1; XM_015784872.1. [Q6AT32-2]
DR   AlphaFoldDB; Q6AT32; -.
DR   SMR; Q6AT32; -.
DR   STRING; 4530.OS05T0559600-01; -.
DR   CAZy; GT43; Glycosyltransferase Family 43.
DR   PaxDb; Q6AT32; -.
DR   PRIDE; Q6AT32; -.
DR   EnsemblPlants; Os05t0559600-01; Os05t0559600-01; Os05g0559600. [Q6AT32-1]
DR   GeneID; 4339589; -.
DR   Gramene; Os05t0559600-01; Os05t0559600-01; Os05g0559600. [Q6AT32-1]
DR   KEGG; osa:4339589; -.
DR   eggNOG; KOG1476; Eukaryota.
DR   HOGENOM; CLU_044006_0_0_1; -.
DR   InParanoid; Q6AT32; -.
DR   OMA; HTGLHEV; -.
DR   OrthoDB; 901158at2759; -.
DR   PlantReactome; R-OSA-5654909; Xylan biosynthesis.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   Genevisible; Q6AT32; OS.
DR   GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015018; F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; IEA:InterPro.
DR   GO; GO:0042285; F:xylosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0010417; P:glucuronoxylan biosynthetic process; IBA:GO_Central.
DR   GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IBA:GO_Central.
DR   GO; GO:0045492; P:xylan biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00218; GlcAT-I; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR005027; Glyco_trans_43.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR10896; PTHR10896; 1.
DR   Pfam; PF03360; Glyco_transf_43; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell wall biogenesis/degradation; Glycoprotein;
KW   Glycosyltransferase; Golgi apparatus; Membrane; Reference proteome;
KW   Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..451
FT                   /note="Probable beta-1,4-xylosyltransferase GT43E"
FT                   /id="PRO_0000407559"
FT   TOPO_DOM        1..88
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        89..109
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..451
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   CARBOHYD        260
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        366
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         21..59
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_040957"
SQ   SEQUENCE   451 AA;  51324 MW;  064E350177211403 CRC64;
     MVSSRRNTGG IQRDGSLRDW SEFVDPSPSP KLLYSQSYVA MRGLLSSLVS MDFALLSSRL
     KSAWAAILSQ RHTRSPERSK SRGLSCKRLA FHLFVCFMVG IFIGFMPFFS VDVSQKIVSE
     NGRLPFDEGA VDRGMVDGKV KELETIVVEK EVDIIDESEV EESPPVPAML DDEADFVESA
     PAIPDINDLD ITVRKLLIIV TITTVRPQQA YYLNRLAHVL KTVQSPLLWL VVEWPDQSFQ
     TAEILRSSGV MYRHLICRKN TTSVRKIAVC QRNTAIYHIK KHRLDGIMHF ADEERSYMSD
     VFEEMRKIRR FGAWPVAIHT GIKYRVVLEG PICKGNRVTG WNTIQNIQKK SAVRRFPVGF
     SGFAFNSTML WDPERWNRPP MDSVIVHSGG RGGLQESRFI EKLVKHERQI EGLPEDCNRV
     MVWNFNLEPP LLNVPPGWSL HKNLDAVIPV T