GT4A_SOLAA
ID GT4A_SOLAA Reviewed; 491 AA.
AC Q5H861;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Nuatigenin 3-beta-glucosyltransferase {ECO:0000305};
DE EC=2.4.1.192 {ECO:0000269|PubMed:15821879};
DE AltName: Full=Glycosyltransferase 4A {ECO:0000303|PubMed:15821879};
DE Short=SaGT4A {ECO:0000303|PubMed:15821879};
GN Name=GT4A {ECO:0000303|PubMed:15821879};
OS Solanum aculeatissimum (Dutch eggplant) (Solanum khasianum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=267265;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=15821879; DOI=10.1007/s11103-004-7204-2;
RA Kohara A., Nakajima C., Hashimoto K., Ikenaga T., Tanaka H., Shoyama Y.,
RA Yoshida S., Muranaka T.;
RT "A novel glucosyltransferase involved in steroid saponin biosynthesis in
RT Solanum aculeatissimum.";
RL Plant Mol. Biol. 57:225-239(2005).
CC -!- FUNCTION: Glucosyltransferase involved in steroid saponin biosynthesis
CC (PubMed:15821879). Catalyzes the 3-O-glucosylation of steroidal
CC sapogenins, such as diosgenin, nuatigenin and tigogenin
CC (PubMed:15821879). Can glucosylate steroidal alkaloids, such as
CC solanidine, solasodine and tomatidine (PubMed:15821879).
CC {ECO:0000269|PubMed:15821879}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=nuatigenin + UDP-alpha-D-glucose = H(+) + nuatigenin 3-beta-D-
CC glucopyranoside + UDP; Xref=Rhea:RHEA:19329, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15574, ChEBI:CHEBI:15575, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.192;
CC Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19330;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diosgenin + UDP-alpha-D-glucose = diosgenin 3-O-beta-D-
CC glucoside + H(+) + UDP; Xref=Rhea:RHEA:61888, ChEBI:CHEBI:4629,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:74020; Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61889;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tigogenin + UDP-alpha-D-glucose = H(+) + tigogenin 3-O-beta-D-
CC glucopyranoside + UDP; Xref=Rhea:RHEA:66276, ChEBI:CHEBI:9595,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885,
CC ChEBI:CHEBI:166995; Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66277;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=solasodine + UDP-alpha-D-glucose = H(+) + solasodine 3-beta-D-
CC glucoside + UDP; Xref=Rhea:RHEA:61844, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:145042,
CC ChEBI:CHEBI:145043; Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61845;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=solanidine + UDP-alpha-D-glucose = H(+) + solanidine 3-O-beta-
CC D-glucopyranoside + UDP; Xref=Rhea:RHEA:66280, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:166996,
CC ChEBI:CHEBI:166997; Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66281;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tomatidine + UDP-alpha-D-glucose = H(+) + tomatidine 3-O-beta-
CC D-glucopyranoside + UDP; Xref=Rhea:RHEA:66284, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:166998,
CC ChEBI:CHEBI:166999; Evidence={ECO:0000269|PubMed:15821879};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66285;
CC Evidence={ECO:0000269|PubMed:15821879};
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems and leaves.
CC {ECO:0000269|PubMed:15821879}.
CC -!- INDUCTION: Down-regulated after wounding.
CC {ECO:0000269|PubMed:15821879}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB182385; BAD89042.1; -; mRNA.
DR AlphaFoldDB; Q5H861; -.
DR SMR; Q5H861; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR BioCyc; MetaCyc:MON18C3-13; -.
DR GO; GO:0047248; F:nuatigenin 3-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102202; F:soladodine glucosyltransferase activity; IEA:RHEA.
DR GO; GO:0035251; F:UDP-glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016135; P:saponin biosynthetic process; IDA:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..491
FT /note="Nuatigenin 3-beta-glucosyltransferase"
FT /id="PRO_0000452210"
SQ SEQUENCE 491 AA; 55328 MW; 330F3A0965280C35 CRC64;
MDNGSNQLHV LFLPYFATGH IIPLVNAARL FVFHAGVKVT ILTTHHNASL FRSTIDNDVE
DGHSVISIHT LRFPSTEVGL PEGIENFSSA SSPELAGKVF YAIYLLQKPM EDKIREIHPD
CIFSDMYLPW TVNIALELKI PRLLFNQSSY MYNSILYNLR LYKPHKSKTI TSTDSISVPG
LPDKIEFKLS QLTDDLIKPE DEKNAFDELL DRTRESEDRS YGIVHDTFYE LEPAYADYYQ
KVKKTKCWQI GPISHFSSKL FRRKELINAV DESNSCAIVE WLNEQEHKSV LYVSFGSVVR
FPEAQLTEIA KALEASSIPF IWVVKKDQSA ETTCLLEEEK LKNKGLIIRG WAPQLTILDH
SAVGGFMTHC GWNSILEAII AGVPLVTWPV FAEQFYNEKL VEVMGLGVKV GAEVHESNGG
VEISSLVIES EKIKEAIEKL MDDSKESQKI REKVIGMSEM AKNAVEEGGS SWNNLTALID
DIKNFTSTTN V
