ID   GTCS_ANEMI              Reviewed;         370 AA.
AC   Q44930;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Sensor protein GtcS;
DE            EC=2.7.13.3;
GN   Name=gtcS;
OS   Aneurinibacillus migulanus (Bacillus migulanus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae;
OC   Aneurinibacillus group; Aneurinibacillus.
OX   NCBI_TaxID=47500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 9999 / DSM 2895 / JCM 8504 / NBRC 15520 / NCIMB 7096 / NCTC
RC   7096;
RX   PubMed=7579582; DOI=10.3109/10425179509030982;
RA   Turgay K., Marahiel M.A.;
RT   "The gtcRS operon coding for two-component system regulatory proteins is
RT   located adjacent to the grs operon of Bacillus brevis.";
RL   DNA Seq. 5:283-290(1995).
CC   -!- FUNCTION: Member of the two-component regulatory system GtcS/GtcR which
CC       may act in the control of the transcription of the grs operon which
CC       encodes the multienzymes involved in the biosynthesis of the peptide
CC       antibiotic gramicidin S. Probably activates GtcR by phosphorylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; X78502; CAA55265.1; -; Genomic_DNA.
DR   PIR; I40085; I40085.
DR   AlphaFoldDB; Q44930; -.
DR   SMR; Q44930; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00304; HAMP; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   4: Predicted;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Transferase; Transmembrane; Transmembrane helix;
KW   Two-component regulatory system.
FT   CHAIN           1..370
FT                   /note="Sensor protein GtcS"
FT                   /id="PRO_0000074769"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          66..118
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          133..355
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   370 AA;  42758 MW;  740D53F3006718CE CRC64;
     MITAYILFTV TVGVTNSIVF YLDDHIGTRH FKKRVYCSSA WIVAWRLMEM VIFALSVYLY
     SRWTSKRITG PLEKITDAIQ KMREGEFAQR LCFKADYELT LIQEHFNEMV AHLEKTEAEK
     NKLEQSKQRM LLDLSHDFKT PITTIQGYAM ALQMGVVDSP EKQRRYLEMI YNKSIIVTAL
     VEDMFQLATL DSPDLPSAEE HGRFGRTGSE IAIAYFDQFE QNKFSLDLKI PTQRVMIRMN
     RNLLYRALSN LLSNTLKHNP KGTKVALSLT DTHEAILLEV MDNGIGIAEE LKESIFQPFV
     RGDKARTGEG TGLGLPSPKK RLNFMVESCC SRVNQGKQYS RSFSQRLEND LPYRLPYHRM
     NKFHRNANQQ