ID   GTF1_LEUME              Reviewed;         284 AA.
AC   B2MUU6;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Dextransucrase 1;
DE            EC=2.4.1.5;
DE   AltName: Full=Glucansucrase 1;
DE   AltName: Full=Sucrose 6-glucosyltransferase 1;
DE   Flags: Fragment;
GN   Name=dsrF;
OS   Leuconostoc mesenteroides.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=1245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CMG713;
RA   Sarwat F.;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   CHARACTERISTICS OF DEXTRAN.
RC   STRAIN=CMG713;
RX   PubMed=18953402; DOI=10.7150/ijbs.4.379;
RA   Sarwat F., Ul Qader S.A., Aman A., Ahmed N.;
RT   "Production & characterization of a unique dextran from an indigenous
RT   Leuconostoc mesenteroides CMG713.";
RL   Int. J. Biol. Sci. 4:379-386(2008).
CC   -!- FUNCTION: Involved in the production of dextran, an extracellular
CC       glucan polymer. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC         glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC         COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC         ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC   -!- MISCELLANEOUS: The dextran produced by Leuconostoc mesenteroides CMG713
CC       has a molecular mass of about 2000 kDa and contains exclusively alpha-
CC       (1->6) glycosidic linkages in the main chain.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000305}.
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DR   EMBL; EU621844; ACC65964.1; -; Genomic_DNA.
DR   AlphaFoldDB; B2MUU6; -.
DR   SMR; B2MUU6; -.
DR   CAZy; GH70; Glycoside Hydrolase Family 70.
DR   BRENDA; 2.4.1.5; 839.
DR   GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046527; F:glucosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:InterPro.
DR   InterPro; IPR003318; Glyco_hydro70cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF02324; Glyco_hydro_70; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           <1..>284
FT                   /note="Dextransucrase 1"
FT                   /id="PRO_0000403441"
FT   NON_TER         1
FT   NON_TER         284
SQ   SEQUENCE   284 AA;  30936 MW;  F6228D1BE8189CF0 CRC64;
     DTVPRVYYAD LYTDDGQYMA TKSPFFDAIN TLLKARVQYV AGGQSMSVDS NDVLTSVRYG
     KNAMTASDTG TSETRTEGVG VIVSNNAELQ LEDGHTVTLH MGAAHKNQAY RALLSTTADG
     LAYYDTDENA PVAYTDANGD LIFTNESIYG VQNPQVSGYL AVWVPVGAQQ DQDARTASDT
     TTNTSDKVFH SNAALDSQVI YEGFSNFQAF ATDSSEYTNV VIAQNADQFK QWGVTSFQLA
     PQYRSSTDTS FLDSIIQNGY AFTDRYDLGY GTPTQYGAVD HVRA