GTF3_LEUME
ID GTF3_LEUME Reviewed; 6 AA.
AC P85080;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 1.
DT 02-JUN-2021, entry version 20.
DE RecName: Full=Dextransucrase;
DE EC=2.4.1.5;
DE AltName: Full=Glucansucrase;
DE AltName: Full=Sucrose 6-glucosyltransferase;
DE Flags: Fragment;
OS Leuconostoc mesenteroides.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Leuconostoc.
OX NCBI_TaxID=1245;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=AA1 {ECO:0000269|Ref.1};
RA Aman A., Ul Qader S.A., Azhar A., Syed M.N.;
RT "Purification and characterization of glucansucrase from Leuconostoc
RT mesentriodes AA1.";
RL Submitted (JAN-2007) to UniProtKB.
CC -!- FUNCTION: Involved in the production of dextran, an extracellular
CC glucan polymer. {ECO:0000269|Ref.1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC Evidence={ECO:0000269|Ref.1};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=69.88 mM for sucrose {ECO:0000269|Ref.1};
CC pH dependence:
CC Optimum pH is 5.0. {ECO:0000269|Ref.1};
CC Temperature dependence:
CC Optimum temperature is 35 degrees Celsius. {ECO:0000269|Ref.1};
CC -!- MISCELLANEOUS: Synthesizes water-soluble glucans (alpha 1,6-glucose).
CC {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000255}.
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DR BRENDA; 2.4.1.5; 839.
DR GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosyltransferase; Transferase.
FT CHAIN 1..>6
FT /note="Dextransucrase"
FT /id="PRO_0000284553"
FT NON_TER 6
FT /evidence="ECO:0000303|Ref.1"
SQ SEQUENCE 6 AA; 636 MW; 72D1A451B5BAC000 CRC64;
DSTNTV