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GTF3_LEUME
ID   GTF3_LEUME              Reviewed;           6 AA.
AC   P85080;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 1.
DT   02-JUN-2021, entry version 20.
DE   RecName: Full=Dextransucrase;
DE            EC=2.4.1.5;
DE   AltName: Full=Glucansucrase;
DE   AltName: Full=Sucrose 6-glucosyltransferase;
DE   Flags: Fragment;
OS   Leuconostoc mesenteroides.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=1245;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RC   STRAIN=AA1 {ECO:0000269|Ref.1};
RA   Aman A., Ul Qader S.A., Azhar A., Syed M.N.;
RT   "Purification and characterization of glucansucrase from Leuconostoc
RT   mesentriodes AA1.";
RL   Submitted (JAN-2007) to UniProtKB.
CC   -!- FUNCTION: Involved in the production of dextran, an extracellular
CC       glucan polymer. {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC         glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC         COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC         ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC         Evidence={ECO:0000269|Ref.1};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=69.88 mM for sucrose {ECO:0000269|Ref.1};
CC       pH dependence:
CC         Optimum pH is 5.0. {ECO:0000269|Ref.1};
CC       Temperature dependence:
CC         Optimum temperature is 35 degrees Celsius. {ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: Synthesizes water-soluble glucans (alpha 1,6-glucose).
CC       {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000255}.
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DR   BRENDA; 2.4.1.5; 839.
DR   GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosyltransferase; Transferase.
FT   CHAIN           1..>6
FT                   /note="Dextransucrase"
FT                   /id="PRO_0000284553"
FT   NON_TER         6
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   6 AA;  636 MW;  72D1A451B5BAC000 CRC64;
     DSTNTV
 
 
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