GTFC_STRMU
ID GTFC_STRMU Reviewed; 1455 AA.
AC P13470; O69382; O69385; O69388; O69391; O69397; P05427;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2002, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Glucosyltransferase-SI;
DE Short=GTF-SI;
DE EC=2.4.1.5;
DE AltName: Full=Dextransucrase;
DE AltName: Full=Sucrose 6-glucosyltransferase;
DE Flags: Precursor;
GN Name=gtfC; OrderedLocusNames=SMU_1005;
OS Streptococcus mutans serotype c (strain ATCC 700610 / UA159).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=210007;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GS-5;
RX PubMed=2976010; DOI=10.1016/0378-1119(88)90382-4;
RA Ueda S., Shiroza T., Kuramitsu H.K.;
RT "Sequence analysis of the gtfC gene from Streptococcus mutans GS-5.";
RL Gene 69:101-109(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f,
RC MT4467 / Serotype e, and MT8148 / Serotype c;
RX PubMed=9570124; DOI=10.1111/j.1574-6968.1998.tb12965.x;
RA Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S.,
RA Kimura S., Hamada S.;
RT "Molecular analyses of glucosyltransferase genes among strains of
RT Streptococcus mutans.";
RL FEMS Microbiol. Lett. 161:331-336(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700610 / UA159;
RX PubMed=12397186; DOI=10.1073/pnas.172501299;
RA Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B.,
RA Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S.,
RA Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.;
RT "Genome sequence of Streptococcus mutans UA159, a cariogenic dental
RT pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-349.
RC STRAIN=GS-5;
RX PubMed=3040685; DOI=10.1128/jb.169.9.4263-4270.1987;
RA Shiroza T., Ueda S., Kuramitsu H.K.;
RT "Sequence analysis of the gtfB gene from Streptococcus mutans.";
RL J. Bacteriol. 169:4263-4270(1987).
CC -!- FUNCTION: Production of extracellular glucans, that are thought to play
CC a key role in the development of the dental plaque because of their
CC ability to adhere to smooth surfaces and mediate the aggregation of
CC bacterial cells and food debris.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- MISCELLANEOUS: GTF-I synthesizes water-insoluble glucans (alpha 1,3-
CC linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble
CC glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000305}.
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DR EMBL; M22054; AAA88592.1; -; Genomic_DNA.
DR EMBL; D88652; BAA26102.1; -; Genomic_DNA.
DR EMBL; D88655; BAA26106.1; -; Genomic_DNA.
DR EMBL; D88658; BAA26110.1; -; Genomic_DNA.
DR EMBL; D88661; BAA26114.1; -; Genomic_DNA.
DR EMBL; D89978; BAA26120.1; -; Genomic_DNA.
DR EMBL; AE014133; AAN58706.1; -; Genomic_DNA.
DR EMBL; M17361; AAA88589.1; -; Genomic_DNA.
DR PIR; JT0345; JT0345.
DR RefSeq; NP_721400.1; NC_004350.2.
DR RefSeq; WP_002352269.1; NC_004350.2.
DR PDB; 3AIB; X-ray; 3.09 A; A/B/C/D/E/F/G/H=244-1087.
DR PDB; 3AIC; X-ray; 3.11 A; A/B/C/D/E/F/G/H=244-1087.
DR PDB; 3AIE; X-ray; 2.10 A; A/B/C/D/E/F/G/H=244-1087.
DR PDBsum; 3AIB; -.
DR PDBsum; 3AIC; -.
DR PDBsum; 3AIE; -.
DR AlphaFoldDB; P13470; -.
DR SMR; P13470; -.
DR STRING; 210007.SMU_1005; -.
DR ChEMBL; CHEMBL2366466; -.
DR CAZy; GH70; Glycoside Hydrolase Family 70.
DR PRIDE; P13470; -.
DR EnsemblBacteria; AAN58706; AAN58706; SMU_1005.
DR KEGG; smu:SMU_1005; -.
DR PATRIC; fig|210007.7.peg.898; -.
DR eggNOG; COG0366; Bacteria.
DR eggNOG; COG5263; Bacteria.
DR HOGENOM; CLU_001623_1_0_9; -.
DR OMA; NSKGEWF; -.
DR PhylomeDB; P13470; -.
DR SABIO-RK; P13470; -.
DR EvolutionaryTrace; P13470; -.
DR Proteomes; UP000002512; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
DR GO; GO:0046527; F:glucosyltransferase activity; IEA:InterPro.
DR GO; GO:0009250; P:glucan biosynthetic process; IEA:InterPro.
DR InterPro; IPR018337; Cell_wall/Cho-bd_repeat.
DR InterPro; IPR027636; Glucan-bd_rpt.
DR InterPro; IPR003318; Glyco_hydro70cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR022263; KxYKxGKxW.
DR Pfam; PF01473; Choline_bind_1; 1.
DR Pfam; PF19127; Choline_bind_3; 4.
DR Pfam; PF02324; Glyco_hydro_70; 1.
DR Pfam; PF19258; KxYKxGKxW_sig; 1.
DR SUPFAM; SSF51445; SSF51445; 2.
DR TIGRFAMs; TIGR04035; glucan_65_rpt; 4.
DR TIGRFAMs; TIGR03715; KxYKxGKxW; 1.
DR PROSITE; PS51170; CW; 11.
PE 1: Evidence at protein level;
KW 3D-structure; Dental caries; Glycosyltransferase; Reference proteome;
KW Repeat; Secreted; Signal; Transferase.
FT SIGNAL 1..34
FT CHAIN 35..1455
FT /note="Glucosyltransferase-SI"
FT /id="PRO_0000021386"
FT REPEAT 184..203
FT /note="Cell wall-binding 1"
FT REPEAT 205..224
FT /note="Cell wall-binding 2"
FT REPEAT 1116..1135
FT /note="Cell wall-binding 3"
FT REPEAT 1136..1155
FT /note="Cell wall-binding 4"
FT REPEAT 1219..1239
FT /note="Cell wall-binding 5"
FT REPEAT 1242..1262
FT /note="Cell wall-binding 6"
FT REPEAT 1263..1282
FT /note="Cell wall-binding 7"
FT REPEAT 1307..1327
FT /note="Cell wall-binding 8"
FT REPEAT 1328..1347
FT /note="Cell wall-binding 9"
FT REPEAT 1372..1392
FT /note="Cell wall-binding 10"
FT REPEAT 1393..1412
FT /note="Cell wall-binding 11"
FT REGION 64..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..1050
FT /note="Catalytic; approximate"
FT VARIANT 21
FT /note="V -> I (in strain: GS-5)"
FT VARIANT 81
FT /note="P -> L (in strain: MT4239)"
FT VARIANT 106
FT /note="D -> V (in strain: GS-5)"
FT VARIANT 116
FT /note="S -> A (in strain: GS-5 and MT4467)"
FT VARIANT 126
FT /note="A -> T (in strain: GS-5)"
FT VARIANT 150..151
FT /note="SR -> PK (in strain: GS-5, MT4239 and MT4467)"
FT VARIANT 256
FT /note="A -> V (in strain: GS-5 and MT4467)"
FT VARIANT 425
FT /note="R -> N (in strain: MT4251)"
FT VARIANT 519
FT /note="Y -> D (in strain: MT4245 and MT4251)"
FT VARIANT 538
FT /note="R -> K (in strain: MT4245 and MT4251)"
FT VARIANT 545
FT /note="Y -> F (in strain: MT4245 and MT4251)"
FT VARIANT 597
FT /note="N -> D (in strain: MT4245, MT4251, MT4467 and
FT MT8148)"
FT VARIANT 600
FT /note="R -> K (in strain: MT4245, MT4251, MT4467 and
FT MT8148)"
FT VARIANT 601
FT /note="A -> T (in strain: GS-5)"
FT VARIANT 614
FT /note="M -> T (in strain: GS-5)"
FT VARIANT 727
FT /note="T -> I (in strain: MT8148)"
FT VARIANT 734
FT /note="A -> V (in strain: MT8148)"
FT VARIANT 964
FT /note="L -> F (in strain: MT4239)"
FT VARIANT 1113
FT /note="N -> Y (in strain: MT4239)"
FT VARIANT 1118
FT /note="A -> T (in strain: MT4239)"
FT VARIANT 1204
FT /note="I -> V (in strain: GS-5, MT4239, MT4467 and MT8148)"
FT VARIANT 1208
FT /note="V -> I (in strain: MT8148)"
FT VARIANT 1292..1294
FT /note="DGH -> NGY (in strain: GS-5, MT4467 and sMT8148)"
FT VARIANT 1305..1369
FT /note="Missing (in strain: MT4245)"
FT VARIANT 1326
FT /note="I -> V (in strain: MT8148)"
FT VARIANT 1331
FT /note="T -> A (in strain: GS-5, MT4239, MT4467 and MT8148)"
FT VARIANT 1377
FT /note="R -> K (in strain: MT8148)"
FT VARIANT 1398
FT /note="V -> I (in strain: MT8148)"
FT VARIANT 1424
FT /note="D -> N (in strain: MT4239)"
FT VARIANT 1439
FT /note="V -> I (in strain: MT4239 and MT8148)"
FT VARIANT 1444
FT /note="S -> P (in strain: MT8148)"
FT CONFLICT 1337..1455
FT /note="QRLYFKSNGVQAKGELITERKGRIKYYDPNSGNEVRNRYVRTSSGNWYYFGN
FT DGYALIGWHVVEGRRVYFDENGVYRYASHDQRNHWDYDYRRDFGRGSSSAVRFRHSRNG
FT FFDNFFRF -> HASILSLMVFRLRESSLQSVKVVSNTMILIPEMKFVIVM (in
FT Ref. 1; AAA88592/BAA26102)"
FT /evidence="ECO:0000305"
FT HELIX 246..249
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 256..258
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 274..279
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 280..282
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 283..286
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 295..297
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 303..316
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 329..350
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 354..366
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 368..370
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 372..375
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 386..389
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 394..399
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 411..414
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 434..438
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 443..457
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 459..464
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 467..469
FT /evidence="ECO:0007829|PDB:3AIB"
FT STRAND 473..476
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 479..481
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 485..498
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 500..502
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 504..508
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 521..528
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 530..535
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 537..546
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 551..553
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 559..562
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 563..566
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 569..571
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 580..582
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 589..591
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 592..602
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 614..629
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 634..636
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 640..647
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 651..658
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 659..662
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 665..668
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 669..671
FT /evidence="ECO:0007829|PDB:3AIC"
FT HELIX 677..690
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 695..702
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 705..712
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 726..729
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 733..738
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 751..754
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 757..759
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 763..770
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 772..777
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 782..787
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 795..799
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 801..803
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 807..811
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 813..821
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 849..852
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 856..858
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 870..872
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 874..880
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 882..887
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 892..894
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 908..910
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 911..913
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 915..919
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 924..928
FT /evidence="ECO:0007829|PDB:3AIC"
FT HELIX 935..947
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 951..956
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 959..961
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 965..975
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 988..997
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 1000..1002
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 1003..1007
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 1008..1011
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 1012..1018
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 1020..1024
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 1028..1030
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 1045..1047
FT /evidence="ECO:0007829|PDB:3AIE"
FT STRAND 1048..1052
FT /evidence="ECO:0007829|PDB:3AIE"
FT TURN 1065..1068
FT /evidence="ECO:0007829|PDB:3AIE"
FT HELIX 1075..1077
FT /evidence="ECO:0007829|PDB:3AIB"
FT HELIX 1082..1085
FT /evidence="ECO:0007829|PDB:3AIE"
SQ SEQUENCE 1455 AA; 162966 MW; 3CB455A99A4FEC86 CRC64;
MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE SQASLVTTSE
AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA NTANFDVKPT TTSEQSKTDN
SDKIIATSKA VNRLTATGKF VPANNNTAHS RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA
LGNVKNIRKV NGKYYYYKED GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND
NTNSFAQYNQ VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW
PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE KNTNWLRQTI
SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN SNYRILNRTP TNQTGKKDPR
YTADRTIGGY EFLLANDVDN SNPVVQAEQL NWLHFLMNFG NIYANDPDAN FDSIRVDAVD
NVDADLLQIA GDYLKAAKGI HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR
LSLLYSLAKP LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR
AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN KSSVPRVYYG
DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ QVGNSEIITS VRYGKGALKA
TDTGDRTTRT SGVAVIEGNN PSLRLKASDR VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS
DQEAAGLVRY TNDRGELIFT AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST
DGKSVHQNAA LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV
SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI KVMADWVPDQ
MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG KDQQAKYGGA FLEELQAKYP
ELFARKQIST GVPMDPSVKI KQWSAKYFNG TNILGRGAGY VLKDQATNTY FSLVSDNTFL
PKSLVNPNHG TSSSVTGLVF DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ
SINGANYYFL SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG
LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK GEWFLFDHNG
VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP NSGNEVRNRF VRNSKGEWFL
FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG ELITERKGRI KYYDPNSGNE VRNRYVRTSS
GNWYYFGNDG YALIGWHVVE GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR
FRHSRNGFFD NFFRF
