GTFD_STRMU
ID GTFD_STRMU Reviewed; 1462 AA.
AC P49331; O69383; O69386; O69389; O69392; O69398;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2002, sequence version 3.
DT 25-MAY-2022, entry version 144.
DE RecName: Full=Glucosyltransferase-S;
DE Short=GTF-S;
DE EC=2.4.1.5;
DE AltName: Full=Dextransucrase;
DE AltName: Full=Sucrose 6-glucosyltransferase;
DE Flags: Precursor;
GN Name=gtfD; OrderedLocusNames=SMU_910;
OS Streptococcus mutans serotype c (strain ATCC 700610 / UA159).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=210007;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GS-5;
RX PubMed=2148600; DOI=10.1099/00221287-136-10-2099;
RA Honda O., Kato C., Kuramitsu H.K.;
RT "Nucleotide sequence of the Streptococcus mutans gtfD gene encoding the
RT glucosyltransferase-S enzyme.";
RL J. Gen. Microbiol. 136:2099-2105(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f,
RC MT4467 / Serotype e, and MT8148 / Serotype c;
RX PubMed=9570124; DOI=10.1111/j.1574-6968.1998.tb12965.x;
RA Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S.,
RA Kimura S., Hamada S.;
RT "Molecular analyses of glucosyltransferase genes among strains of
RT Streptococcus mutans.";
RL FEMS Microbiol. Lett. 161:331-336(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700610 / UA159;
RX PubMed=12397186; DOI=10.1073/pnas.172501299;
RA Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B.,
RA Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S.,
RA Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J.;
RT "Genome sequence of Streptococcus mutans UA159, a cariogenic dental
RT pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002).
CC -!- FUNCTION: Production of extracellular glucans, that are thought to play
CC a key role in the development of the dental plaque because of their
CC ability to adhere to smooth surfaces and mediate the aggregation of
CC bacterial cells and food debris.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- MISCELLANEOUS: GTF-I synthesizes water-insoluble glucans (alpha 1,3-
CC linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble
CC glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M29296; AAA26895.1; -; Genomic_DNA.
DR EMBL; D88653; BAA26103.1; -; Genomic_DNA.
DR EMBL; D88656; BAA26107.1; -; Genomic_DNA.
DR EMBL; D88659; BAA26111.1; -; Genomic_DNA.
DR EMBL; D88662; BAA26115.1; -; Genomic_DNA.
DR EMBL; D89979; BAA26121.1; -; Genomic_DNA.
DR EMBL; AE014133; AAN58619.1; -; Genomic_DNA.
DR RefSeq; NP_721313.1; NC_004350.2.
DR RefSeq; WP_002352262.1; NC_004350.2.
DR AlphaFoldDB; P49331; -.
DR SMR; P49331; -.
DR STRING; 210007.SMU_910; -.
DR ChEMBL; CHEMBL3822352; -.
DR CAZy; GH70; Glycoside Hydrolase Family 70.
DR PRIDE; P49331; -.
DR EnsemblBacteria; AAN58619; AAN58619; SMU_910.
DR KEGG; smu:SMU_910; -.
DR PATRIC; fig|210007.7.peg.813; -.
DR eggNOG; COG0366; Bacteria.
DR eggNOG; COG5263; Bacteria.
DR HOGENOM; CLU_001623_1_0_9; -.
DR OMA; DKSIRYF; -.
DR PhylomeDB; P49331; -.
DR SABIO-RK; P49331; -.
DR Proteomes; UP000002512; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
DR GO; GO:0046527; F:glucosyltransferase activity; IEA:InterPro.
DR GO; GO:0009250; P:glucan biosynthetic process; IEA:InterPro.
DR Gene3D; 2.60.40.1180; -; 1.
DR InterPro; IPR018337; Cell_wall/Cho-bd_repeat.
DR InterPro; IPR027636; Glucan-bd_rpt.
DR InterPro; IPR003318; Glyco_hydro70cat.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR022263; KxYKxGKxW.
DR Pfam; PF01473; Choline_bind_1; 1.
DR Pfam; PF19127; Choline_bind_3; 4.
DR Pfam; PF02324; Glyco_hydro_70; 1.
DR Pfam; PF19258; KxYKxGKxW_sig; 1.
DR SUPFAM; SSF51445; SSF51445; 2.
DR TIGRFAMs; TIGR04035; glucan_65_rpt; 5.
DR TIGRFAMs; TIGR03715; KxYKxGKxW; 1.
DR PROSITE; PS51170; CW; 14.
PE 3: Inferred from homology;
KW Dental caries; Glycosyltransferase; Reference proteome; Repeat; Secreted;
KW Signal; Transferase.
FT SIGNAL 1..?
FT /evidence="ECO:0000255"
FT CHAIN ?..1462
FT /note="Glucosyltransferase-S"
FT /id="PRO_0000021387"
FT REPEAT 171..190
FT /note="Cell wall-binding 1"
FT REPEAT 192..211
FT /note="Cell wall-binding 2"
FT REPEAT 1095..1115
FT /note="Cell wall-binding 3"
FT REPEAT 1116..1136
FT /note="Cell wall-binding 4"
FT REPEAT 1137..1156
FT /note="Cell wall-binding 5"
FT REPEAT 1180..1201
FT /note="Cell wall-binding 6"
FT REPEAT 1202..1221
FT /note="Cell wall-binding 7"
FT REPEAT 1223..1244
FT /note="Cell wall-binding 8"
FT REPEAT 1246..1266
FT /note="Cell wall-binding 9"
FT REPEAT 1267..1286
FT /note="Cell wall-binding 10"
FT REPEAT 1310..1330
FT /note="Cell wall-binding 11"
FT REPEAT 1331..1350
FT /note="Cell wall-binding 12"
FT REPEAT 1352..1372
FT /note="Cell wall-binding 13"
FT REPEAT 1374..1394
FT /note="Cell wall-binding 14"
FT REGION 35..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..63
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..133
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 10
FT /note="Y -> H (in strain: GS-5, MT4239, MT4245, MT4251,
FT MT4467 and MT8148)"
FT VARIANT 19
FT /note="I -> V (in strain: GS-5, MT4239, MT4245, MT4251,
FT MT4467 and MT8148)"
FT VARIANT 58
FT /note="K -> E (in strain: MT4467)"
FT VARIANT 68
FT /note="A -> S (in strain: MT4239 and MT4245)"
FT VARIANT 81
FT /note="A -> T (in strain: MT4251 and MT8148)"
FT VARIANT 113
FT /note="T -> I (in strain: MT4239 and MT4245)"
FT VARIANT 122
FT /note="A -> V (in strain: MT4239, MT4245 and MT8148)"
FT VARIANT 132
FT /note="A -> S (in strain: GS-5 and MT4467)"
FT VARIANT 135
FT /note="A -> V (in strain: MT4245)"
FT VARIANT 137
FT /note="A -> T (in strain: GS-5, MT4239, MT4245, MT4251,
FT MT4467 and MT8148)"
FT VARIANT 202
FT /note="V -> L (in strain: MT4239)"
FT VARIANT 255
FT /note="D -> N (in strain: MT8148)"
FT VARIANT 275
FT /note="E -> D (in strain: MT4239, MT4245 and MT4251)"
FT VARIANT 288
FT /note="D -> N (in strain: MT4239, MT4245 and MT4251)"
FT VARIANT 301
FT /note="Q -> H (in strain: MT4245)"
FT VARIANT 313
FT /note="D -> N (in strain: MT4239 and MT4251)"
FT VARIANT 317
FT /note="E -> K (in strain: MT4239)"
FT VARIANT 328
FT /note="V -> F (in strain: MT4239)"
FT VARIANT 350
FT /note="F -> L (in strain: MT4239, MT4251 and MT4467)"
FT VARIANT 628..633
FT /note="KKKYTQ -> EKEYTL (in strain: MT4251)"
FT VARIANT 688
FT /note="A -> S (in strain: MT4239)"
FT VARIANT 726..732
FT /note="TDQGSEA -> ADKGNDS (in strain: MT4251)"
FT VARIANT 726..730
FT /note="TDQGS -> ADKGN (in strain: MT4239 and MT4245)"
FT VARIANT 762
FT /note="T -> A (in strain: GS-5, MT4239, MT4245, MT4251,
FT MT4467 and MT8148)"
FT VARIANT 964
FT /note="D -> Y (in strain: MT4251)"
FT VARIANT 1019
FT /note="E -> K (in strain: MT4245 and MT4251)"
FT VARIANT 1059..1060
FT /note="LG -> IR (in strain: MT4251)"
FT VARIANT 1060
FT /note="G -> R (in strain: MT4245)"
FT VARIANT 1080
FT /note="G -> R (in strain: MT4239)"
FT VARIANT 1142
FT /note="H -> Q (in strain: GS-5)"
FT VARIANT 1198
FT /note="S -> N (in strain: MT4239)"
FT VARIANT 1220
FT /note="Y -> C (in strain: MT4251 and MT4467)"
FT VARIANT 1280
FT /note="F -> L (in strain: MT4467)"
FT VARIANT 1282
FT /note="Q -> P (in strain: MT4245)"
FT VARIANT 1290
FT /note="K -> T (in strain: MT4245)"
FT VARIANT 1311
FT /note="N -> D (in strain: MT4245)"
FT VARIANT 1403
FT /note="G -> D (in strain: GS-5 and MT4467)"
FT VARIANT 1425
FT /note="G -> R (in strain: GS-5)"
FT VARIANT 1449
FT /note="R -> K (in strain: MT4467)"
FT CONFLICT 1428..1462
FT /note="RYYDKNSGNMVYNKVVTLANGRRIGIDRWGIARYY -> VYR (in Ref.
FT 1; AAA26895)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1462 AA; 163388 MW; CE4A279C4D708645 CRC64;
METKRRYKMY KVKKHWVTIA VASGLITLGT TTLGSSVSAE TEQQTSDKVV TQKSEDDKAA
SESSQTDAPK TKQAQTEQTQ AQSQANVADT STSITKETPS QNITTQANSD DKTVTNTKSE
EAQTSEERTK QAEEAQATAS SQALTQAKAE LTKQRQTAAQ ENKNPVDLAA IPNVKQIDGK
YYYIGSDGQP KKNFALTVNN KVLYFDKNTG ALTDTSQYQF KQGLTKLNND YTPHNQIVNF
ENTSLETIDN YVTADSWYRP KDILKNGKTW TASSESDLRP LLMSWWPDKQ TQIAYLNYMN
QQGLGTGENY TADSSQESLN LAAQTVQVKI ETKISQTQQT QWLRDIINSF VKTQPNWNSQ
TESDTSAGEK DHLQGGALLY SNSDKTAYAN SDYRLLNRTP TSQTGKPKYF EDNSSGGYDF
LLANDIDNSN PVVQAEQLNW LHYLMNYGSI VANDPEANFD GVRVDAVDNV NADLLQIASD
YLKAHYGVDK SEKNAINHLS ILEAWSDNDP QYNKDTKGAQ LPIDNKLRLS LLYALTRPLE
KDASNKNEIR SGLEPVITNS LNNRSAEGKN SERMANYIFI RAHDSEVQTV IAKIIKAQIN
PKTDGLTFTL DELKQAFKIY NEDMRQAKKK YTQSNIPTAY ALMLSNKDSI TRLYYGDMYS
DDGQYMATKS PYYDAIDTLL KARIKYAAGG QDMKITYVEG DKSHMDWDYT GVLTSVRYGT
GANEATDQGS EATKTQGMAV ITSNNPSLKL NQNDKVIVNM GTAHKNQEYR PLLLTTKDGL
TSYTSDAAAK SLYRKTNDKG ELVFDASDIQ GYLNPQVSGY LAVWVPVGAS DNQDVRVAAS
NKANATGQVY ESSSALDSQL IYEGFSNFQD FVTKDSDYTN KKIAQNVQLF KSWGVTSFEM
APQYVSSEDG SFLDSIIQNG YAFEDRYDLA MSKNNKYGSQ QDMINAVKAL HKSGIQVIAD
WVPDQIYNLP GKEVVTATRV NDYGEYRKDS EIKNTLYAAN TKSNGKDYQA KYGGAFLSEL
AAKYPSIFNR TQISNGKKID PSEKITAWKA KYFNGTNILG RGVGYVLKDN ASDKYFELKG
NQTYLPKQMT NKEASTGFVN DGNGMTFYST SGYQAKNSFV QDAKGNWYYF DNNGHMVYGL
QHLNGEVQYF LSNGVQLRES FLENADGSKN YFGHLGNRYS NGYYSFDNDS KWRYFDASGV
MAVGLKTING NTQYFDQDGY QVKGAWITGS DGKKRYFDDG SGNMAVNRFA NDKNGDWYYL
NSDGIALVGV QTINGKTYYF GQDGKQIKGK IITDNGKLKY FLANSGELAR NIFATDSQNN
WYYFGSDGVA VTGSQTIAGK KLYFASDGKQ VKGSFVTYNG KVHYYHADSG ELQVNRFEAD
KDGNWYYLDS NGEALTGSQR INGQRVFFTR EGKQVKGDVA YDERGLLRYY DKNSGNMVYN
KVVTLANGRR IGIDRWGIAR YY
