ID   GTFE_AMYOR              Reviewed;         409 AA.
AC   Q9AFC6;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Glycosyltransferase GtfE;
DE            EC=2.4.1.-;
GN   Name=gtfE;
OS   Amycolatopsis orientalis (Nocardia orientalis).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Amycolatopsis.
OX   NCBI_TaxID=31958;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=ATCC 19795 / DSM 40040 / CBS 547.68 / JCM 4235 / KCTC 9412 / NBRC
RC   12806 / NCIMB 12945 / M43-05865;
RX   PubMed=11294642; DOI=10.1021/bi010050w;
RA   Losey H.C., Peczuh M.W., Chen Z., Eggert U.S., Dong S.D., Pelczer I.,
RA   Kahne D., Walsh C.T.;
RT   "Tandem action of glycosyltransferases in the maturation of vancomycin and
RT   teicoplanin aglycones: novel glycopeptides.";
RL   Biochemistry 40:4745-4755(2001).
RN   [2]
RP   FUNCTION.
RX   PubMed=9115410; DOI=10.1016/s1074-5521(97)90288-x;
RA   Solenberg P.J., Matsushima P., Stack D.R., Wilkie S.C., Thompson R.C.,
RA   Baltz R.H.;
RT   "Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces
RT   toyocaensis.";
RL   Chem. Biol. 4:195-202(1997).
CC   -!- FUNCTION: D-glucosyltransferase that acts on the aglycone core,
CC       transferring D-glucose to the phenolic hydroxyl of OH-Phegly(4) to form
CC       a devancoaminyl-vancomycin (DVV) intermediate in the biosynthesis of
CC       glycopeptide antibiotic vancomycin. Also able to glycosylate A47934, an
CC       antibiotic with a teicoplanin-like heptapeptide, but lacking sugar
CC       residues. {ECO:0000269|PubMed:11294642, ECO:0000269|PubMed:9115410}.
CC   -!- PATHWAY: Antibiotic biosynthesis; vancomycin biosynthesis.
CC       {ECO:0000269|PubMed:11294642}.
CC   -!- MISCELLANEOUS: In A.orientalis different glycosyltransferases are
CC       involved in biosynthesis of the vancomycin group of antibiotics. GtfA
CC       (AC P96558), GtfB (AC P96559) and GtfC (AC P96560) are involved in
CC       biosynthesis of antibiotic chloroeremomycin, while GtfD (AC Q9AFC7) and
CC       GtfE are involved in biosynthesis of vancomycin.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
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DR   EMBL; AF351622; AAK31353.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AFC6; -.
DR   SMR; Q9AFC6; -.
DR   STRING; 1125971.ASJB01000062_gene4404; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   eggNOG; COG1819; Bacteria.
DR   UniPathway; UPA00162; -.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR   GO; GO:0033072; P:vancomycin biosynthetic process; IDA:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR004276; GlycoTrans_28_N.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF03033; Glyco_transf_28; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..409
FT                   /note="Glycosyltransferase GtfE"
FT                   /id="PRO_0000430440"
SQ   SEQUENCE   409 AA;  43139 MW;  6D3A8C83E1679F0B CRC64;
     MRVLLSTCGS RGDVEPLVAL AVRLRARGAE VRMCAPPDCA DRLAEVGVPH LPLGRSARPA
     AAGEAKPLTA EDMLRFTTET IAMQFDRIPA AAEGCDAVVT TGLLAAALGV RSVAEKLGIP
     YFYAFHCPSY VPSPYYAPPP PLGEPPAPEG TDIRALWERN NQSAFRRYGA PLKSQRAAIG
     LPPVEDIFEH GYTDHPWMAA DQVLAPLQPT DLDAVQTGAW ILPDERPLSP EMEAFLDAGT
     PPVYLGFGSL RAPADAAKVA IEAIRAHGRR VILSRGWADL VLPDDRDDCF ATGEVNQQVL
     FRRVAAVIHH GGAGTTHVAT RAGAPQILVP QIADQPYYAG RVAELGIGVA HDGPTPTFES
     LSAALTTALA PETRVRAEAV AGTVLTDGAA AAADLLFAAV GEEKPAVPA