ID   GTPB3_DANRE             Reviewed;         500 AA.
AC   Q501Z5;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=tRNA modification GTPase GTPBP3, mitochondrial;
DE   AltName: Full=GTP-binding protein 3;
DE   Flags: Precursor;
GN   Name=gtpbp3; ORFNames=zgc:112394;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: GTPase involved in the 5-carboxymethylaminomethyl
CC       modification (mnm(5)s(2)U34) of the wobble uridine base in
CC       mitochondrial tRNAs. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC       GTPase superfamily. TrmE GTPase family. {ECO:0000305}.
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DR   EMBL; BC095801; AAH95801.1; -; mRNA.
DR   RefSeq; NP_001018605.1; NM_001020769.1.
DR   AlphaFoldDB; Q501Z5; -.
DR   SMR; Q501Z5; -.
DR   STRING; 7955.ENSDARP00000004190; -.
DR   PaxDb; Q501Z5; -.
DR   GeneID; 553807; -.
DR   KEGG; dre:553807; -.
DR   CTD; 84705; -.
DR   ZFIN; ZDB-GENE-050522-335; gtpbp3.
DR   eggNOG; KOG1191; Eukaryota.
DR   InParanoid; Q501Z5; -.
DR   OrthoDB; 834238at2759; -.
DR   PhylomeDB; Q501Z5; -.
DR   ChiTaRS; gtpbp3; zebrafish.
DR   PRO; PR:Q501Z5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IDA:ZFIN.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0048568; P:embryonic organ development; IMP:ZFIN.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   CDD; cd04164; trmE; 1.
DR   Gene3D; 1.20.120.430; -; 1.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00379; GTPase_MnmE; 1.
DR   InterPro; IPR031168; G_TrmE.
DR   InterPro; IPR006073; GTP-bd.
DR   InterPro; IPR018948; GTP-bd_TrmE_N.
DR   InterPro; IPR004520; GTPase_MnmE.
DR   InterPro; IPR027368; MnmE_dom2.
DR   InterPro; IPR025867; MnmE_helical.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   Pfam; PF12631; MnmE_helical; 1.
DR   Pfam; PF10396; TrmE_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00450; mnmE_trmE_thdF; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51709; G_TRME; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Mitochondrion; Nucleotide-binding; Reference proteome;
KW   Transit peptide; tRNA processing.
FT   TRANSIT         1..73
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           74..500
FT                   /note="tRNA modification GTPase GTPBP3, mitochondrial"
FT                   /id="PRO_0000280268"
FT   DOMAIN          248..422
FT                   /note="TrmE-type G"
FT   BINDING         255..262
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         302..306
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         373..376
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   500 AA;  54836 MW;  E44A5C4F2EE7069A CRC64;
     MHFISCCLRR STVSIAVSRS LLVKRLFCSG WPGDDTIYSL SSGQGKCGVA VVRVSGPASA
     LAVRRLTRSL PAPRTASLRS ISHPQSKELL DRGLVLWFPG PASFTGEDSA EFHIHGGPAV
     ISGVLQALGS LPGLRPAEAG EFTRRAFYAG KLDLTEVEGL SDLIHAETEA QRRQALRQMA
     GDLGRIYQDW TDQLKRCLAH VEAFIDFSED ELIEDGVLND VDRAVQQLQT DMENHLSDER
     RGERLRSGVH VVIAGSTNAG KSSLLNLLTQ RPAAIVSPTA GTTRDVLEVP LDIGGYPVLL
     SDTAGLRDTS DSVEQEGVRR ARQRVEQADL SLVVVDLTQL PSERRHVPVF LRGHLKNILE
     RSSQQQQHIL ILNESDLVSA EQQRSIQTSL QELSGAPSAC FLSCHSRDGL EELLTLLHNT
     LKTLCGDPLI GSPTLTQTRH RTHLQKSIEA LQQYHEYRDV DLALAAEGLR LGLLSLGRIT
     GRVSPEEILD VIFRDFCIGK