GTPB3_MOUSE
ID GTPB3_MOUSE Reviewed; 492 AA.
AC Q923K4; Q58E73; Q99M99;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=tRNA modification GTPase GTPBP3, mitochondrial;
DE AltName: Full=GTP-binding protein 3;
DE Flags: Precursor;
GN Name=Gtpbp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RX PubMed=14680828; DOI=10.1016/j.bbrc.2003.10.187;
RA Li X., Guan M.-X.;
RT "Identification and characterization of mouse GTPBP3 gene encoding a
RT mitochondrial GTP-binding protein involved in tRNA modification.";
RL Biochem. Biophys. Res. Commun. 312:747-754(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: GTPase involved in the 5-carboxymethylaminomethyl
CC modification (mnm(5)s(2)U34) of the wobble uridine base in
CC mitochondrial tRNAs. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14680828}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q923K4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q923K4-2; Sequence=VSP_023585, VSP_023586;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. Highly expressed in tissues
CC with high metabolic rates including heart, liver and brain. Weakly
CC expressed in skeletal muscle. {ECO:0000269|PubMed:14680828}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. TrmE GTPase family. {ECO:0000305}.
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DR EMBL; AF361482; AAK35216.2; -; mRNA.
DR EMBL; AY029613; AAK60410.1; -; Genomic_DNA.
DR EMBL; BC092042; AAH92042.1; -; mRNA.
DR CCDS; CCDS40384.1; -. [Q923K4-1]
DR RefSeq; NP_115933.2; NM_032544.3. [Q923K4-1]
DR AlphaFoldDB; Q923K4; -.
DR SMR; Q923K4; -.
DR BioGRID; 214000; 1.
DR STRING; 10090.ENSMUSP00000007754; -.
DR PhosphoSitePlus; Q923K4; -.
DR EPD; Q923K4; -.
DR MaxQB; Q923K4; -.
DR PaxDb; Q923K4; -.
DR PRIDE; Q923K4; -.
DR ProteomicsDB; 271059; -. [Q923K4-1]
DR ProteomicsDB; 271060; -. [Q923K4-2]
DR Antibodypedia; 27650; 110 antibodies from 22 providers.
DR DNASU; 70359; -.
DR Ensembl; ENSMUST00000007754; ENSMUSP00000007754; ENSMUSG00000007610. [Q923K4-1]
DR Ensembl; ENSMUST00000095259; ENSMUSP00000092892; ENSMUSG00000007610. [Q923K4-2]
DR Ensembl; ENSMUST00000150969; ENSMUSP00000114193; ENSMUSG00000007610. [Q923K4-1]
DR GeneID; 70359; -.
DR KEGG; mmu:70359; -.
DR UCSC; uc009mdn.1; mouse. [Q923K4-1]
DR CTD; 84705; -.
DR MGI; MGI:1917609; Gtpbp3.
DR VEuPathDB; HostDB:ENSMUSG00000007610; -.
DR eggNOG; KOG1191; Eukaryota.
DR GeneTree; ENSGT00390000016851; -.
DR HOGENOM; CLU_019624_3_1_1; -.
DR InParanoid; Q923K4; -.
DR OMA; CEIQCHG; -.
DR PhylomeDB; Q923K4; -.
DR TreeFam; TF313153; -.
DR BioGRID-ORCS; 70359; 13 hits in 74 CRISPR screens.
DR ChiTaRS; Gtpbp3; mouse.
DR PRO; PR:Q923K4; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q923K4; protein.
DR Bgee; ENSMUSG00000007610; Expressed in ectoplacental cone and 192 other tissues.
DR ExpressionAtlas; Q923K4; baseline and differential.
DR Genevisible; Q923K4; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR CDD; cd04164; trmE; 1.
DR Gene3D; 1.20.120.430; -; 1.
DR Gene3D; 3.30.1360.120; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00379; GTPase_MnmE; 1.
DR InterPro; IPR031168; G_TrmE.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR018948; GTP-bd_TrmE_N.
DR InterPro; IPR004520; GTPase_MnmE.
DR InterPro; IPR027368; MnmE_dom2.
DR InterPro; IPR025867; MnmE_helical.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR Pfam; PF12631; MnmE_helical; 1.
DR Pfam; PF10396; TrmE_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00450; mnmE_trmE_thdF; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51709; G_TRME; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; GTP-binding; Mitochondrion; Nucleotide-binding;
KW Reference proteome; Transit peptide; tRNA processing.
FT TRANSIT 1..81
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 82..492
FT /note="tRNA modification GTPase GTPBP3, mitochondrial"
FT /id="PRO_0000280266"
FT DOMAIN 249..416
FT /note="TrmE-type G"
FT BINDING 256..263
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 303..307
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 374..377
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT VAR_SEQ 198..241
FT /note="ALAYVEAYIDFGEDDNLEEGVLEQADREVRALEVALGSHLRDAR -> RTEK
FT FEPWRWRWVPTCEMLGVDKGSSRGQMLWSLDLPMRARAVW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023585"
FT VAR_SEQ 242..492
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023586"
SQ SEQUENCE 492 AA; 52175 MW; 85BB8A5E38F93597 CRC64;
MWRGLSALVT QAAWAPLRLC ARCSTSAESL VPSSTIFALS SGQGRCAIAV IRTSGPASGL
ALRSLTALQE PPPARRACLR LLRHPCSGEP LDRSLVLWFP GPQSFTGEDC VEFHVHGGPA
VVSGVLQALG SVPGLRPAEA GEFTRRAFAH GKLSLTEVEG LADLIRAETE AQRRQALRQL
DGELSQLCQG WAKTLTKALA YVEAYIDFGE DDNLEEGVLE QADREVRALE VALGSHLRDA
RRGQRLLSGA NVVVTGPPNA GKSSLVNLLS QKPVSIVSPE PGTTRDVLET PVDLAGFPVL
LSDTAGLREG VGAVEQEGVR RARHRLEQAD IILGVLDASD LASSSSCSFL DTVVTPLLAQ
SQDSGGQRLL LLLNKSDLLS ANAPACDIAL PPHLLLSCHT GAGMDSLLQA LKTELAAVCG
DPSTGPPLLT RVRHQYHLQG CLDALGHYQL ATDLALAAEA LRQARRQLNH LTGGGGTEEI
LDLIFQDFCV GK
