ID   GTPB3_RAT               Reviewed;         492 AA.
AC   Q5PQQ1;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=tRNA modification GTPase GTPBP3, mitochondrial;
DE   AltName: Full=GTP-binding protein 3;
DE   Flags: Precursor;
GN   Name=Gtpbp3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: GTPase involved in the 5-carboxymethylaminomethyl
CC       modification (mnm(5)s(2)U34) of the wobble uridine base in
CC       mitochondrial tRNAs. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC       GTPase superfamily. TrmE GTPase family. {ECO:0000305}.
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DR   EMBL; BC087083; AAH87083.1; -; mRNA.
DR   RefSeq; NP_001011919.1; NM_001011919.1.
DR   AlphaFoldDB; Q5PQQ1; -.
DR   SMR; Q5PQQ1; -.
DR   STRING; 10116.ENSRNOP00000057102; -.
DR   PhosphoSitePlus; Q5PQQ1; -.
DR   PaxDb; Q5PQQ1; -.
DR   PRIDE; Q5PQQ1; -.
DR   Ensembl; ENSRNOT00000060355; ENSRNOP00000057102; ENSRNOG00000023403.
DR   GeneID; 290633; -.
DR   KEGG; rno:290633; -.
DR   UCSC; RGD:1305367; rat.
DR   CTD; 84705; -.
DR   RGD; 1305367; Gtpbp3.
DR   eggNOG; KOG1191; Eukaryota.
DR   GeneTree; ENSGT00390000016851; -.
DR   HOGENOM; CLU_019624_3_1_1; -.
DR   InParanoid; Q5PQQ1; -.
DR   OMA; CEIQCHG; -.
DR   OrthoDB; 834238at2759; -.
DR   PhylomeDB; Q5PQQ1; -.
DR   TreeFam; TF313153; -.
DR   PRO; PR:Q5PQQ1; -.
DR   Proteomes; UP000002494; Chromosome 16.
DR   Bgee; ENSRNOG00000023403; Expressed in skeletal muscle tissue and 20 other tissues.
DR   Genevisible; Q5PQQ1; RN.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   CDD; cd04164; trmE; 1.
DR   Gene3D; 1.20.120.430; -; 1.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00379; GTPase_MnmE; 1.
DR   InterPro; IPR031168; G_TrmE.
DR   InterPro; IPR006073; GTP-bd.
DR   InterPro; IPR018948; GTP-bd_TrmE_N.
DR   InterPro; IPR004520; GTPase_MnmE.
DR   InterPro; IPR027368; MnmE_dom2.
DR   InterPro; IPR025867; MnmE_helical.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   Pfam; PF12631; MnmE_helical; 1.
DR   Pfam; PF10396; TrmE_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00450; mnmE_trmE_thdF; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51709; G_TRME; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Mitochondrion; Nucleotide-binding; Reference proteome;
KW   Transit peptide; tRNA processing.
FT   TRANSIT         1..81
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           82..492
FT                   /note="tRNA modification GTPase GTPBP3, mitochondrial"
FT                   /id="PRO_0000280267"
FT   DOMAIN          249..416
FT                   /note="TrmE-type G"
FT   BINDING         256..263
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         303..307
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         374..377
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   492 AA;  52017 MW;  5749762D7E3FBA61 CRC64;
     MWRGLSALVT RPASAPLRLC ARCSTGAESL VPGSTIFALS SGQGRCAIAV IRTSGPASGL
     ALRSLTALRE PPPARSACLR LLRHPCSGEP LDRSLVLWFP GPQSFTGEDC MELHVHGGPA
     VVSGVLQALG SVPGLRPAKA GEFTRRAFAH GKLSLTEVEG LADLIHAETE AQRRQALRQL
     DGELSQLCQG WAKTLTKALA HVEAYIDFGE DDNLEEGVLE QVDRDVRALE VALSSHLRDA
     RRGQRLRSGA NVVVAGPPNA GKSSLVNLLS QKPVSIVSPE PGTTRDILET PVDLAGFPVL
     LSDTAGLREG AGAVEQEGVR RARQRLEQAD IILGMLDASD LASSSSCSFL DTVVAPLVAQ
     SHDSGRQRLL LLLNKSDLLS ANAPASSTAL PPHLLLSCHT GAGMDALLQA LKTELAAVCG
     DPSTGPPLLT RARHQYHLQG CLDALGRFQL TTDLALAAEA LRQARRQLSH LTGGGGTEEI
     LDLIFQDFCV GK