GTPB4_RAT
ID GTPB4_RAT Reviewed; 637 AA.
AC Q99P77;
DT 24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=GTP-binding protein 4;
DE AltName: Full=Chronic renal failure gene protein;
DE AltName: Full=Nucleolar GTP-binding protein 1;
GN Name=Gtpbp4; Synonyms=Crfg, Nog1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX PubMed=11316846; DOI=10.1681/asn.v125883;
RA Laping N.J., Olson B.A., Zhu Y.;
RT "Identification of a novel nuclear guanosine triphosphate-binding protein
RT differentially expressed in renal disease.";
RL J. Am. Soc. Nephrol. 12:883-890(2001).
CC -!- FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit.
CC {ECO:0000250|UniProtKB:Q9BZE4}.
CC -!- SUBUNIT: Associates with pre-60S ribosomal particles. Interacts with
CC MINAS-60 (product of an alternative open reading frame of RBM10).
CC {ECO:0000250|UniProtKB:Q9BZE4}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9BZE4}.
CC -!- SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase
CC superfamily. OBG GTPase family. NOG subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU01047}.
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DR EMBL; AF325355; AAK13446.1; -; mRNA.
DR AlphaFoldDB; Q99P77; -.
DR SMR; Q99P77; -.
DR IntAct; Q99P77; 1.
DR STRING; 10116.ENSRNOP00000064860; -.
DR jPOST; Q99P77; -.
DR PRIDE; Q99P77; -.
DR UCSC; RGD:620783; rat.
DR RGD; 620783; Gtpbp4.
DR eggNOG; KOG1490; Eukaryota.
DR InParanoid; Q99P77; -.
DR PhylomeDB; Q99P77; -.
DR PRO; PR:Q99P77; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:HGNC-UCL.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:HGNC-UCL.
DR GO; GO:0005525; F:GTP binding; ISS:HGNC-UCL.
DR GO; GO:0003924; F:GTPase activity; ISS:HGNC-UCL.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ISO:RGD.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:HGNC-UCL.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:HGNC-UCL.
DR GO; GO:0022408; P:negative regulation of cell-cell adhesion; ISS:HGNC-UCL.
DR GO; GO:0033342; P:negative regulation of collagen binding; ISS:HGNC-UCL.
DR GO; GO:0008156; P:negative regulation of DNA replication; ISS:HGNC-UCL.
DR GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:HGNC-UCL.
DR GO; GO:0050821; P:protein stabilization; ISS:HGNC-UCL.
DR GO; GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; ISS:HGNC-UCL.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031167; G_OBG.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR024926; NOG1.
DR InterPro; IPR041623; NOG1_N.
DR InterPro; IPR010674; NOG1_Rossman_fold_dom.
DR InterPro; IPR012973; NOG_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF06858; NOG1; 1.
DR Pfam; PF17835; NOG1_N; 1.
DR Pfam; PF08155; NOGCT; 1.
DR PIRSF; PIRSF038919; NOG1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51710; G_OBG; 1.
PE 2: Evidence at transcript level;
KW Acetylation; GTP-binding; Isopeptide bond; Nucleotide-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Ribosome biogenesis; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT CHAIN 2..637
FT /note="GTP-binding protein 4"
FT /id="PRO_0000195025"
FT DOMAIN 169..340
FT /note="OBG-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT REGION 499..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 525..637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..543
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 572..592
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 605..625
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 175..182
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 221..225
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 289..292
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT MOD_RES 103
FT /note="N6-acetyllysine; alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT MOD_RES 122
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT MOD_RES 559
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT CROSSLNK 103
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT CROSSLNK 332
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
FT CROSSLNK 535
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9BZE4"
SQ SEQUENCE 637 AA; 74288 MW; 5CBF5FA48140F702 CRC64;
MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD
RLSQILSDFP KLDDIHPFYA DLMNILYDKD HYKLALGQIN IAKNLVDNVA KDYVRLMKYG
DSLYRCKQLK RAALGRMCTI IKRQRQSLEY LEQVRQHLSR LPTIDPNTRT LLLCGYPNVG
KSSFINKVTR ADVDVQPYAF TTKSLFVGHV DYKYLRWQVV DTPGILDHPL EDRNTIEMQA
ITALAHLRAA VLYVMDLSEQ CGHGLKEQLG LFQNIRPLFI NKPLIVVASK CEVKRIAELS
EEDQKIFLDL QAEGFPVIET STLTEEGVIQ VKTEACDRLL AHRVETKMKG NKVNEVLNRL
HLAVPNKRDD KERPPFIPEG VVARRKRMEI AEPKKKRERD LELEMGDDYI LDLQKYWDLM
NSSEKYDKIP EIWEGHNAAD YIDPAIMKKL EELEKGKKSS EQLLGSMPVS LRVKTRKWWK
IRQLAKQIRE KKKLKILQSK EKNTQGPRMP RTAKKVQRAD LENEMRSLGV DMDDKDNAHY
AVRARRSRSV TRKRKREESV PPSSIARSRS RSCSKTPRDV SGLRDVKMVK KAKTMMKKAQ
KKMNRLGKKG EADRHVFDMK PKHLLSGKRK AGKKERR
