GTPB8_HUMAN
ID GTPB8_HUMAN Reviewed; 284 AA.
AC Q8N3Z3; A6NE99; A6NN11; A8K0P6; Q5I0Y4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=GTP-binding protein 8;
GN Name=GTPBP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain cortex, and Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- INTERACTION:
CC Q8N3Z3; Q9UJX2: CDC23; NbExp=3; IntAct=EBI-6912536, EBI-396137;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8N3Z3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N3Z3-2; Sequence=VSP_034055;
CC Name=3;
CC IsoId=Q8N3Z3-3; Sequence=VSP_034056, VSP_034057;
CC -!- MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngB GTPase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK289611; BAF82300.1; -; mRNA.
DR EMBL; AK292651; BAF85340.1; -; mRNA.
DR EMBL; AC074044; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW79655.1; -; Genomic_DNA.
DR EMBL; BC010137; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC037163; AAH37163.1; -; mRNA.
DR CCDS; CCDS33820.1; -. [Q8N3Z3-1]
DR CCDS; CCDS33821.1; -. [Q8N3Z3-2]
DR RefSeq; NP_054889.2; NM_014170.2. [Q8N3Z3-1]
DR RefSeq; NP_612494.1; NM_138485.1. [Q8N3Z3-2]
DR AlphaFoldDB; Q8N3Z3; -.
DR BioGRID; 118853; 22.
DR IntAct; Q8N3Z3; 11.
DR STRING; 9606.ENSP00000373176; -.
DR iPTMnet; Q8N3Z3; -.
DR PhosphoSitePlus; Q8N3Z3; -.
DR BioMuta; GTPBP8; -.
DR DMDM; 74728797; -.
DR EPD; Q8N3Z3; -.
DR jPOST; Q8N3Z3; -.
DR MassIVE; Q8N3Z3; -.
DR MaxQB; Q8N3Z3; -.
DR PaxDb; Q8N3Z3; -.
DR PeptideAtlas; Q8N3Z3; -.
DR PRIDE; Q8N3Z3; -.
DR ProteomicsDB; 71853; -. [Q8N3Z3-1]
DR ProteomicsDB; 71854; -. [Q8N3Z3-2]
DR ProteomicsDB; 71855; -. [Q8N3Z3-3]
DR Antibodypedia; 52345; 70 antibodies from 15 providers.
DR DNASU; 29083; -.
DR Ensembl; ENST00000295864.9; ENSP00000295864.5; ENSG00000163607.18. [Q8N3Z3-3]
DR Ensembl; ENST00000383677.7; ENSP00000373175.3; ENSG00000163607.18. [Q8N3Z3-2]
DR Ensembl; ENST00000383678.8; ENSP00000373176.2; ENSG00000163607.18. [Q8N3Z3-1]
DR Ensembl; ENST00000485330.5; ENSP00000418877.1; ENSG00000163607.18. [Q8N3Z3-2]
DR GeneID; 29083; -.
DR KEGG; hsa:29083; -.
DR MANE-Select; ENST00000383678.8; ENSP00000373176.2; NM_014170.4; NP_054889.2.
DR UCSC; uc003dzn.4; human. [Q8N3Z3-1]
DR CTD; 29083; -.
DR GeneCards; GTPBP8; -.
DR HGNC; HGNC:25007; GTPBP8.
DR HPA; ENSG00000163607; Low tissue specificity.
DR neXtProt; NX_Q8N3Z3; -.
DR OpenTargets; ENSG00000163607; -.
DR PharmGKB; PA142671705; -.
DR VEuPathDB; HostDB:ENSG00000163607; -.
DR eggNOG; KOG2486; Eukaryota.
DR GeneTree; ENSGT00390000001083; -.
DR HOGENOM; CLU_033732_5_0_1; -.
DR InParanoid; Q8N3Z3; -.
DR OMA; YPFSGLE; -.
DR PhylomeDB; Q8N3Z3; -.
DR TreeFam; TF331089; -.
DR PathwayCommons; Q8N3Z3; -.
DR SignaLink; Q8N3Z3; -.
DR BioGRID-ORCS; 29083; 110 hits in 1085 CRISPR screens.
DR ChiTaRS; GTPBP8; human.
DR GenomeRNAi; 29083; -.
DR Pharos; Q8N3Z3; Tdark.
DR PRO; PR:Q8N3Z3; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q8N3Z3; protein.
DR Bgee; ENSG00000163607; Expressed in secondary oocyte and 212 other tissues.
DR ExpressionAtlas; Q8N3Z3; baseline and differential.
DR Genevisible; Q8N3Z3; HS.
DR GO; GO:0005739; C:mitochondrion; IDA:HPA.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01876; YihA_EngB; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00321; GTPase_EngB; 1.
DR InterPro; IPR030393; G_ENGB_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR019987; GTP-bd_ribosome_bio_YsxC.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03598; GTPase_YsxC; 1.
DR PROSITE; PS51706; G_ENGB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTP-binding; Magnesium; Metal-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..284
FT /note="GTP-binding protein 8"
FT /id="PRO_0000338612"
FT DOMAIN 109..282
FT /note="EngB-type G"
FT BINDING 117..124
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 124
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 146..150
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 148
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 164..167
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 226..229
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 261..263
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT VAR_SEQ 113..145
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_034055"
FT VAR_SEQ 113..121
FT /note="VCFIGRSNV -> LEENIFISG (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034056"
FT VAR_SEQ 122..284
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034057"
FT VARIANT 242
FT /note="Q -> H (in dbSNP:rs1054263)"
FT /id="VAR_048934"
FT CONFLICT 101
FT /note="R -> C (in Ref. 1; BAF82300)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 284 AA; 32147 MW; 446AF4FFBC1E6E65 CRC64;
MAAPGLRLGA GRLFEMPAVL ERLSRYNSTS QAFAEVLRLP KQQLRKLLYP LQEVERFLAP
YGRQDLHLRI FDPSPEDIAR ADNIFTATER NRIDYVSSAV RIDHAPDLPR PEVCFIGRSN
VGKSSLIKAL FSLAPEVEVR VSKKPGHTKK MNFFKVGKHF TVVDMPGYGF RAPEDFVDMV
ETYLKERRNL KRTFLLVDSV VGIQKTDNIA IEMCEEFALP YVIVLTKIDK SSKGHLLKQV
LQIQKFVNMK TQGCFPQLFP VSAVTFSGIH LLRCFIASVT GSLD
