GTPB8_MOUSE
ID GTPB8_MOUSE Reviewed; 285 AA.
AC Q9CY28; Q3KNM0;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=GTP-binding protein 8;
GN Name=Gtpbp8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CY28-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CY28-2; Sequence=VSP_034058;
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngB GTPase family. {ECO:0000305}.
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DR EMBL; AK010979; BAB27301.1; -; mRNA.
DR EMBL; AK147840; BAE28175.1; -; mRNA.
DR EMBL; BC107216; AAI07217.1; -; mRNA.
DR EMBL; BC107217; AAI07218.1; -; mRNA.
DR CCDS; CCDS28188.1; -. [Q9CY28-1]
DR CCDS; CCDS49855.1; -. [Q9CY28-2]
DR RefSeq; NP_001152801.1; NM_001159329.1. [Q9CY28-2]
DR RefSeq; NP_079608.1; NM_025332.3. [Q9CY28-1]
DR AlphaFoldDB; Q9CY28; -.
DR SMR; Q9CY28; -.
DR BioGRID; 211190; 3.
DR STRING; 10090.ENSMUSP00000125352; -.
DR PhosphoSitePlus; Q9CY28; -.
DR EPD; Q9CY28; -.
DR MaxQB; Q9CY28; -.
DR PaxDb; Q9CY28; -.
DR PeptideAtlas; Q9CY28; -.
DR PRIDE; Q9CY28; -.
DR ProteomicsDB; 271061; -. [Q9CY28-1]
DR ProteomicsDB; 271062; -. [Q9CY28-2]
DR Antibodypedia; 52345; 70 antibodies from 15 providers.
DR DNASU; 66067; -.
DR Ensembl; ENSMUST00000023348; ENSMUSP00000023348; ENSMUSG00000022668. [Q9CY28-2]
DR Ensembl; ENSMUST00000162512; ENSMUSP00000125352; ENSMUSG00000022668. [Q9CY28-1]
DR GeneID; 66067; -.
DR KEGG; mmu:66067; -.
DR UCSC; uc007zhq.2; mouse. [Q9CY28-1]
DR UCSC; uc012agd.1; mouse. [Q9CY28-2]
DR CTD; 29083; -.
DR MGI; MGI:1913317; Gtpbp8.
DR VEuPathDB; HostDB:ENSMUSG00000022668; -.
DR eggNOG; KOG2486; Eukaryota.
DR GeneTree; ENSGT00390000001083; -.
DR HOGENOM; CLU_033732_5_0_1; -.
DR InParanoid; Q9CY28; -.
DR OMA; YPFSGLE; -.
DR OrthoDB; 573373at2759; -.
DR PhylomeDB; Q9CY28; -.
DR TreeFam; TF331089; -.
DR BioGRID-ORCS; 66067; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Gtpbp8; mouse.
DR PRO; PR:Q9CY28; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CY28; protein.
DR Bgee; ENSMUSG00000022668; Expressed in right kidney and 210 other tissues.
DR ExpressionAtlas; Q9CY28; baseline and differential.
DR Genevisible; Q9CY28; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01876; YihA_EngB; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00321; GTPase_EngB; 1.
DR InterPro; IPR030393; G_ENGB_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR019987; GTP-bd_ribosome_bio_YsxC.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR03598; GTPase_YsxC; 1.
DR PROSITE; PS51706; G_ENGB; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; GTP-binding; Magnesium; Metal-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..285
FT /note="GTP-binding protein 8"
FT /id="PRO_0000338613"
FT DOMAIN 110..283
FT /note="EngB-type G"
FT BINDING 118..125
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 125
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 147..151
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 149
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 165..168
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 227..230
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 262..264
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT VAR_SEQ 47..113
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034058"
SQ SEQUENCE 285 AA; 31872 MW; D5561D52525253BF CRC64;
MAAARLSHRM GRLLEKAPAL GPWTRVYSTS PAFAEVLRLP QKQLTKVVYP LRELGQHLAA
DSGPGLIEQR LFDPSLEDIG RAESIFEATA RNRIEYLSSA VRLDHAPSLQ QPEVCFIGRS
NVGKSSLIKA LFSLAPDVEV RISKKPGHTK KMNFFKVGKH FTLVDMPGYG YRAPEDFVDM
VETYLKERNN LKRTFLLVDS VVGITKLDNI AIEMCEEFAL PYVMILTKID KSSKGYLLKQ
VLQIQKFVNT QTQGCFPQLF PISAVTNSGV HLLKCFIADI TGSLK
