GTPBA_HUMAN
ID GTPBA_HUMAN Reviewed; 387 AA.
AC A4D1E9; B4DFY6; Q3B7A6; Q5H9V2; Q8IXG8; Q8N982; Q8WU16; Q9BSP1; Q9Y6T6;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=GTP-binding protein 10;
DE AltName: Full=Protein obg homolog 2;
DE Short=ObgH2;
GN Name=GTPBP10; Synonyms=OBGH2; ORFNames=UG0751c10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ILE-368.
RC TISSUE=Fetal brain;
RA Mao Y., Xie Y.;
RT "Isolation of full-length cDNA clones from human fetal brain cDNA
RT library.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP TRP-88.
RC TISSUE=Amygdala, and Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT TRP-88.
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS TRP-88 AND
RP SER-110.
RC TISSUE=Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17054726; DOI=10.1111/j.1365-2443.2006.01017.x;
RA Hirano Y., Ohniwa R.L., Wada C., Yoshimura S.H., Takeyasu K.;
RT "Human small G proteins, ObgH1, and ObgH2, participate in the maintenance
RT of mitochondria and nucleolar architectures.";
RL Genes Cells 11:1295-1304(2006).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: May be involved in the ribosome maturation process.
CC Complements an ObgE(CgtA) function in E.coli ribosome maturation. Plays
CC a role of GTPase in vitro. When missing, disorganization of the
CC nucleolar architecture is observed. {ECO:0000269|PubMed:17054726}.
CC -!- INTERACTION:
CC A4D1E9; Q08379: GOLGA2; NbExp=3; IntAct=EBI-5453796, EBI-618309;
CC A4D1E9; Q5JR59: MTUS2; NbExp=3; IntAct=EBI-5453796, EBI-742948;
CC A4D1E9; Q8ND90: PNMA1; NbExp=4; IntAct=EBI-5453796, EBI-302345;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:17054726}.
CC Chromosome {ECO:0000269|PubMed:17054726}. Note=Found in the dense
CC fibrillar compartment region of the nucleolus. At the onset of mitosis
CC moves to the chromosome surface and remains there until anaphase.
CC Gradually re-assembles into the nucleolus at late anaphase to
CC telophase.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A4D1E9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A4D1E9-2; Sequence=VSP_029880;
CC Name=3;
CC IsoId=A4D1E9-3; Sequence=VSP_029881, VSP_029882;
CC -!- SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase
CC superfamily. OBG GTPase family. {ECO:0000255|PROSITE-ProRule:PRU01047}.
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DR EMBL; AF351613; AAN76513.1; -; mRNA.
DR EMBL; AK095561; BAC04573.1; -; mRNA.
DR EMBL; AK294325; BAG57597.1; -; mRNA.
DR EMBL; CR933597; CAI45922.1; -; mRNA.
DR EMBL; AC002064; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC006153; AAD15550.2; -; Genomic_DNA.
DR EMBL; CH236949; EAL24164.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76880.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76882.1; -; Genomic_DNA.
DR EMBL; BC004923; AAH04923.3; -; mRNA.
DR EMBL; BC021573; AAH21573.2; -; mRNA.
DR EMBL; BC107714; AAI07715.1; -; mRNA.
DR CCDS; CCDS43614.1; -. [A4D1E9-2]
DR CCDS; CCDS5617.1; -. [A4D1E9-1]
DR RefSeq; NP_001036182.1; NM_001042717.2. [A4D1E9-2]
DR RefSeq; NP_149098.2; NM_033107.3. [A4D1E9-1]
DR PDB; 7OI6; EM; 5.70 A; y=1-387.
DR PDBsum; 7OI6; -.
DR AlphaFoldDB; A4D1E9; -.
DR SMR; A4D1E9; -.
DR BioGRID; 124573; 153.
DR IntAct; A4D1E9; 27.
DR MINT; A4D1E9; -.
DR STRING; 9606.ENSP00000222511; -.
DR GlyGen; A4D1E9; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; A4D1E9; -.
DR PhosphoSitePlus; A4D1E9; -.
DR BioMuta; GTPBP10; -.
DR EPD; A4D1E9; -.
DR jPOST; A4D1E9; -.
DR MassIVE; A4D1E9; -.
DR MaxQB; A4D1E9; -.
DR PaxDb; A4D1E9; -.
DR PeptideAtlas; A4D1E9; -.
DR PRIDE; A4D1E9; -.
DR ProteomicsDB; 615; -. [A4D1E9-1]
DR ProteomicsDB; 616; -. [A4D1E9-2]
DR ProteomicsDB; 617; -. [A4D1E9-3]
DR Antibodypedia; 15416; 156 antibodies from 21 providers.
DR DNASU; 85865; -.
DR Ensembl; ENST00000222511.11; ENSP00000222511.7; ENSG00000105793.16. [A4D1E9-1]
DR Ensembl; ENST00000257659.12; ENSP00000257659.8; ENSG00000105793.16. [A4D1E9-2]
DR Ensembl; ENST00000380058.7; ENSP00000369398.3; ENSG00000105793.16. [A4D1E9-3]
DR GeneID; 85865; -.
DR KEGG; hsa:85865; -.
DR MANE-Select; ENST00000222511.11; ENSP00000222511.7; NM_033107.4; NP_149098.2.
DR UCSC; uc003ukm.3; human. [A4D1E9-1]
DR CTD; 85865; -.
DR DisGeNET; 85865; -.
DR GeneCards; GTPBP10; -.
DR HGNC; HGNC:25106; GTPBP10.
DR HPA; ENSG00000105793; Low tissue specificity.
DR MIM; 610920; gene.
DR neXtProt; NX_A4D1E9; -.
DR OpenTargets; ENSG00000105793; -.
DR PharmGKB; PA162390482; -.
DR VEuPathDB; HostDB:ENSG00000105793; -.
DR eggNOG; KOG1489; Eukaryota.
DR GeneTree; ENSGT00940000155589; -.
DR InParanoid; A4D1E9; -.
DR OMA; VFMVDIF; -.
DR OrthoDB; 1150635at2759; -.
DR PhylomeDB; A4D1E9; -.
DR TreeFam; TF314774; -.
DR PathwayCommons; A4D1E9; -.
DR SignaLink; A4D1E9; -.
DR BioGRID-ORCS; 85865; 110 hits in 1082 CRISPR screens.
DR ChiTaRS; GTPBP10; human.
DR GenomeRNAi; 85865; -.
DR Pharos; A4D1E9; Tbio.
DR PRO; PR:A4D1E9; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; A4D1E9; protein.
DR Bgee; ENSG00000105793; Expressed in corpus epididymis and 185 other tissues.
DR ExpressionAtlas; A4D1E9; baseline and differential.
DR Genevisible; A4D1E9; HS.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR CDD; cd01898; Obg; 1.
DR Gene3D; 2.70.210.12; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031167; G_OBG.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR014100; GTP-bd_Obg/CgtA.
DR InterPro; IPR006169; GTP1_OBG_dom.
DR InterPro; IPR036726; GTP1_OBG_dom_sf.
DR InterPro; IPR045086; OBG_GTPase.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11702; PTHR11702; 2.
DR Pfam; PF01018; GTP1_OBG; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR PIRSF; PIRSF002401; GTP_bd_Obg/CgtA; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF82051; SSF82051; 1.
DR PROSITE; PS51710; G_OBG; 1.
DR PROSITE; PS51883; OBG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Chromosome; GTP-binding;
KW Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..387
FT /note="GTP-binding protein 10"
FT /id="PRO_0000312630"
FT DOMAIN 13..148
FT /note="Obg"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01231"
FT DOMAIN 149..344
FT /note="OBG-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 155..162
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 202..206
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 278..281
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT VAR_SEQ 77..155
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.1"
FT /id="VSP_029880"
FT VAR_SEQ 107..116
FT /note="GELNKENDRI -> DSQMLENPLC (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_029881"
FT VAR_SEQ 117..387
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_029882"
FT VARIANT 88
FT /note="C -> W (in dbSNP:rs42663)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005"
FT /id="VAR_037543"
FT VARIANT 110
FT /note="N -> S (in dbSNP:rs42664)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_037544"
FT VARIANT 164
FT /note="L -> F (in dbSNP:rs35001814)"
FT /id="VAR_037545"
FT VARIANT 368
FT /note="M -> I (in dbSNP:rs17863999)"
FT /evidence="ECO:0000269|Ref.1"
FT /id="VAR_037546"
FT CONFLICT 64
FT /note="R -> G (in Ref. 7; AAI07715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 387 AA; 42933 MW; 1A89697B83320075 CRC64;
MVHCSCVLFR KYGNFIDKLR LFTRGGSGGM GYPRLGGEGG KGGDVWVVAQ NRMTLKQLKD
RYPRKRFVAG VGANSKISAL KGSKGKDCEI PVPVGISVTD ENGKIIGELN KENDRILVAQ
GGLGGKLLTN FLPLKGQKRI IHLDLKLIAD VGLVGFPNAG KSSLLSCVSH AKPAIADYAF
TTLKPELGKI MYSDFKQISV ADLPGLIEGA HMNKGMGHKF LKHIERTRQL LFVVDISGFQ
LSSHTQYRTA FETIILLTKE LELYKEELQT KPALLAVNKM DLPDAQDKFH ELMSQLQNPK
DFLHLFEKNM IPERTVEFQH IIPISAVTGE GIEELKNCIR KSLDEQANQE NDALHKKQLL
NLWISDTMSS TEPPSKHAVT TSKMDII
