GTPS2_THYVU
ID GTPS2_THYVU Reviewed; 596 AA.
AC K9Y6Y9; A0A5P8U4C2; K9Y851; V5JZJ2;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 03-AUG-2022, entry version 35.
DE RecName: Full=Putative terpene synthase 2, chloroplastic {ECO:0000303|PubMed:26750479, ECO:0000303|PubMed:31644985};
DE Short=TTPS2 {ECO:0000303|PubMed:31644985};
DE Short=TvTPS2 {ECO:0000303|PubMed:26750479};
DE EC=4.2.3.- {ECO:0000250|UniProtKB:E2E2P0};
DE AltName: Full=Inactive gamma-terpinene synthase 2, chloroplastic {ECO:0000303|PubMed:26750479};
DE Flags: Precursor;
GN Name=TPS2 {ECO:0000303|PubMed:26750479, ECO:0000303|PubMed:31644985};
OS Thymus vulgaris (Thyme).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Thymus.
OX NCBI_TaxID=49992;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RA Rudolph K., Schweininger J., Mueller-Uri F., Kreis W.;
RT "Gamma-terpinene synthase from Thymus vulgaris (GTS).";
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Schimmel J., Krause S.T., Asbach J., Degenhardt J.;
RT "Chemotype regulation in thyme (Thymus vulgaris) is based on terpene
RT synthase expression patterns.";
RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RX PubMed=26750479; DOI=10.1107/s2053230x15023043;
RA Rudolph K., Parthier C., Egerer-Sieber C., Geiger D., Muller Y.A.,
RA Kreis W., Mueller-Uri F.;
RT "Expression, crystallization and structure elucidation of gamma-terpinene
RT synthase from Thymus vulgaris.";
RL Acta Crystallogr. F Struct. Biol. Commun. 72:16-23(2016).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=31644985; DOI=10.1016/j.phytochem.2019.112126;
RA Tohidi B., Rahimmalek M., Arzani A., Trindade H.;
RT "Sequencing and variation of terpene synthase gene (TPS2) as the major gene
RT in biosynthesis of thymol in different Thymus species.";
RL Phytochemistry 169:112126-112126(2020).
CC -!- FUNCTION: Putative monoterpene synthase inactive on geranyl diphosphate
CC (GPP). {ECO:0000250|UniProtKB:E2E2P0}.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:E2E2P0};
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:E2E2P0};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC {ECO:0000250|UniProtKB:E2E2P0};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000250|UniProtKB:E2E2P0}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:A0A0M3Q1Q3}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250|UniProtKB:Q9X839}.
CC -!- MISCELLANEOUS: Missing Asp-356 and Thr-565 residues essential for
CC gamma-terpinene synthase activity. {ECO:0000269|PubMed:26750479}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR EMBL; JQ957865; AFZ41787.1; -; mRNA.
DR EMBL; JX997981; AGS42395.1; -; mRNA.
DR EMBL; JQ957864; AFZ41786.1; -; mRNA.
DR EMBL; MH686200; QFS22625.1; -; mRNA.
DR SMR; K9Y6Y9; -.
DR BRENDA; 4.2.3.114; 12984.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid;
KW Transit peptide.
FT TRANSIT 1..46
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 47..596
FT /note="Putative terpene synthase 2, chloroplastic"
FT /id="PRO_0000453310"
FT REGION 355..361
FT /note="Homodimerization"
FT /evidence="ECO:0000250|UniProtKB:A0A0M3Q1Q3"
FT REGION 427..464
FT /note="Homodimerization"
FT /evidence="ECO:0000250|UniProtKB:A0A0M3Q1Q3"
FT MOTIF 349..353
FT /note="DDXXD motif"
FT /evidence="ECO:0000250|UniProtKB:Q9X839"
FT BINDING 349
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 349
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 353
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 353
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 493
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT BINDING 501
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT CONFLICT 3
FT /note="T -> S (in Ref. 2; AGS42395)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="T -> I (in Ref. 1; AFZ41787)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="E -> K (in Ref. 1; AFZ41787)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="F -> Y (in Ref. 2; AGS42395)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="F -> L (in Ref. 2; AGS42395)"
FT /evidence="ECO:0000305"
FT CONFLICT 523
FT /note="E -> Q (in Ref. 4; QFS22625)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 596 AA; 69023 MW; 06B5CE3F4EB93A98 CRC64;
MATLSMQVST LSKQVKNLNT FGMGSASKLP MVARRVSTTR LRPICSASLQ VEEETRRSGN
YQAPVWNNDF IQSFSTDKYK DEKFLKKKEE LIAQVKVLLN TKMEAVKQLE LIEDLRNLGL
TYYFEDEFKK ILTSIYNEHK GFKNEQVGDL YFTSLAFRLL RLHGFDVSED VFNFFKNEDG
SDFKASLGEN TKDVLELYEA SFLIRVGEVT LEQARVFSTK ILEKKVEEGI KDEKLLAWIQ
HSLALPLHWR IQRLEARWFL DAYKARKDMN PIIYELGKID FHIIQETQLQ EVQEVSQWWT
NTNLAEKLPF VRDRIVECYF WALGLFEPHE YGYQRKMAAI IITFVTIIDD VYDVYGTLDE
LQLFTDAIRK WDVESISTLP YYMQVCYLAV FTYASELAYD ILKDQGFNSI SYLQRSWLSL
VEGFFQEAKW YYAGYTPTLA EYLENAKVSI SSPTIISQVY FTLPNSTERT VVENVFGYHN
ILYLSGMILR LADDLGTTQF ELKRGDVQKA IQCYMNDNNA TEEEGTEHVK YLLREAWQEM
NSAMADPDCP LSEDLVFAAA NLGRASQFIY LDGDGHGVQH SEIHNQMGGL IFEPYV
