GTR12_DANRE
ID GTR12_DANRE Reviewed; 610 AA.
AC Q6NWF1; Q6PHV4;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Solute carrier family 2, facilitated glucose transporter member 12 {ECO:0000305};
DE AltName: Full=Glucose transporter type 12 {ECO:0000303|PubMed:25326603};
DE Short=GLUT-12 {ECO:0000303|PubMed:25326603};
GN Name=slc2a12 {ECO:0000250|UniProtKB:Q8TD20};
GN Synonyms=glut12 {ECO:0000303|PubMed:25326603};
GN ORFNames=zgc:66027 {ECO:0000303|Ref.1}, zgc:85776 {ECO:0000303|Ref.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=25326603; DOI=10.1530/joe-14-0539;
RA Jimenez-Amilburu V., Jong-Raadsen S., Bakkers J., Spaink H.P.,
RA Marin-Juez R.;
RT "GLUT12 deficiency during early development results in heart failure and a
RT diabetic phenotype in zebrafish.";
RL J. Endocrinol. 224:1-15(2015).
CC -!- FUNCTION: Insulin-regulated facilitative glucose transporter.
CC {ECO:0000269|PubMed:25326603}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose(out) = D-glucose(in); Xref=Rhea:RHEA:60376,
CC ChEBI:CHEBI:4167; Evidence={ECO:0000305|PubMed:25326603};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8BFW9};
CC Multi-pass membrane protein {ECO:0000255}. Endomembrane system
CC {ECO:0000250|UniProtKB:Q5J316}; Multi-pass membrane protein
CC {ECO:0000255}. Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q8TD20}. Note=Localizes primarily perinuclear
CC region in the absence of insulin. {ECO:0000250|UniProtKB:Q8TD20}.
CC -!- TISSUE SPECIFICITY: Expressed in the main insulin-sensitive tissues,
CC such as cardiac muscle, skeletal muscle and adipose tissue.
CC {ECO:0000269|PubMed:25326603}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein impairs the
CC development of the embryonic heart resulting in abnormal valve
CC formation (PubMed:25326603). Embryos also show poor glycemic control
CC (PubMed:25326603). {ECO:0000269|PubMed:25326603}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
CC {ECO:0000305}.
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DR EMBL; BC056306; AAH56306.1; -; mRNA.
DR EMBL; BC067616; AAH67616.2; -; mRNA.
DR RefSeq; NP_956832.1; NM_200538.1.
DR RefSeq; XP_005162361.1; XM_005162304.3.
DR AlphaFoldDB; Q6NWF1; -.
DR SMR; Q6NWF1; -.
DR STRING; 7955.ENSDARP00000053530; -.
DR PaxDb; Q6NWF1; -.
DR Ensembl; ENSDART00000053531; ENSDARP00000053530; ENSDARG00000036865.
DR GeneID; 393510; -.
DR KEGG; dre:393510; -.
DR CTD; 154091; -.
DR ZFIN; ZDB-GENE-040426-1513; slc2a12.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000159614; -.
DR InParanoid; Q6NWF1; -.
DR OMA; SCHEQEM; -.
DR OrthoDB; 430696at2759; -.
DR PhylomeDB; Q6NWF1; -.
DR TreeFam; TF332408; -.
DR Reactome; R-DRE-189200; Cellular hexose transport.
DR PRO; PR:Q6NWF1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 23.
DR Bgee; ENSDARG00000036865; Expressed in bone element and 20 other tissues.
DR ExpressionAtlas; Q6NWF1; baseline and differential.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:0072359; P:circulatory system development; IBA:GO_Central.
DR GO; GO:0044381; P:glucose import in response to insulin stimulus; IMP:ZFIN.
DR GO; GO:1904659; P:glucose transmembrane transport; IBA:GO_Central.
DR GO; GO:0060047; P:heart contraction; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0003179; P:heart valve morphogenesis; IMP:ZFIN.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 2.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Glycoprotein; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..610
FT /note="Solute carrier family 2, facilitated glucose
FT transporter member 12"
FT /id="PRO_0000292017"
FT TOPO_DOM 1..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..84
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..325
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..475
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 497..511
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 512..532
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 533..542
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 543..563
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 564..610
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 392
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 429
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 438
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 32
FT /note="G -> S (in Ref. 1; AAH56306)"
FT /evidence="ECO:0000305"
FT CONFLICT 423
FT /note="T -> A (in Ref. 1; AAH56306)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="N -> D (in Ref. 1; AAH56306)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="M -> I (in Ref. 1; AAH56306)"
FT /evidence="ECO:0000305"
FT CONFLICT 597
FT /note="T -> I (in Ref. 1; AAH56306)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 610 AA; 65778 MW; E5F794C4805E757C CRC64;
MDAPEESIRM TSDPQSKIYV QNPDTHIHLE QGPSAKSGNG RALVLCSVSV ACLSGLLMGY
EMSLISGALL QLRDVLTLSC PEQEQVVGSL LLGAFLLSLG GGTILDHYGR RFTIILTALL
CVLGTLLSVC VVSFWALVVG RMLVGMSVAL SGTASCLYAA EVAPAAWRGR CVCVYELMVV
LGMLLGFGLS WAFAGVPDGW RFTFGGALLP ALLQAGVMPL LPDSPRFLLA QQREKEAHAT
LLRLRAGIKE VEPVEDELRA IRLAMGAERL HGFLDLFQSR DNMLQRLLVG AALVFLQQAT
GQPNILAYAS TVLSSVGFHG NEAATLASTG FGVVKVGGTI PAIFLVDKVG PKALLCVGVV
VMMLSTATLG AITMQSRTHV SSLCRGPGNT ANFTLFETGD ETDIQTNTPL GLYQPQNKLK
TNTFLTSIND TREHWILNHT YNHRTALMET AELSKKDSAK IALQSLHEVS PSLKWISLVS
LLVYVAGFSI SLGPMVHVVL SAIFPTGIRG KAVSVISAFN WATNLLISMT FLTLTERIGL
PTVIFSYSAM SFLLVVFVIV FVPETKGRSL EQISKELAMK NHLRGTLLCH RRKHKATAQP
SQEEKALATV
