GTX3B_NEOCU
ID GTX3B_NEOCU Reviewed; 31 AA.
AC P0DV18;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 1.
DT 03-AUG-2022, entry version 3.
DE RecName: Full=M-poneritoxin-Nc3b {ECO:0000303|PubMed:34302796};
DE Short=M-PONTX-Nc3b {ECO:0000303|PubMed:34302796};
DE AltName: Full=Poneratoxin {ECO:0000305};
DE AltName: Full=Ponericin Nc3b {ECO:0000303|PubMed:34302796};
OS Neoponera commutata (Large hunting ant) (Pachycondyla commutata).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Ponerinae; Ponerini; Pachycondyla.
OX NCBI_TaxID=613619;
RN [1]
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, SYNTHESIS, TOXIC
RP DOSE, BIOASSAY, AND MUTAGENESIS OF ALA-1; THR-3; GLU-5; LEU-19; GLY-20;
RP MET-21; SER-23 AND LEU-30.
RC TISSUE=Venom;
RX PubMed=34302796; DOI=10.1016/j.bcp.2021.114693;
RA Nixon S.A., Robinson S.D., Agwa A.J., Walker A.A., Choudhary S.,
RA Touchard A., Undheim E.A.B., Robertson A., Vetter I., Schroeder C.I.,
RA Kotze A.C., Herzig V., King G.F.;
RT "Multipurpose peptides: the venoms of Amazonian stinging ants contain
RT anthelmintic ponericins with diverse predatory and defensive activities.";
RL Biochem. Pharmacol. 192:114693-114693(2021).
CC -!- FUNCTION: Membrane-perturbating peptide with a few moderate activities
CC (PubMed:34302796). It is insecticidal, since it induces reversible
CC paralysis in insects (L.cuprina) after 1 hour, but fails to kill them
CC (PubMed:34302796). It is also antiparasitic, since it moderately
CC inhibits the larval development of the major pathogenic nematode of
CC ruminants (H.contortus, IC(50)=23.2 uM) and reduces the motility of
CC adult males of the other nematode B.malayi (PubMed:34302796). It does
CC not show antibacterial activity (MIC>40 uM) (PubMed:34302796). It is
CC not cytotoxic to HEK293 cells and does not induce hemolysis in human
CC erythrocytes (PubMed:34302796). It does not cause an increase in
CC intracellular calcium concentration on neuronal and epithelial cell
CC lines (PubMed:34302796). {ECO:0000269|PubMed:34302796}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34302796}. Target
CC cell membrane {ECO:0000305|PubMed:34302796}. Note=Adopts an alpha-
CC helical conformation in membrane-mimetic environments.
CC {ECO:0000305|PubMed:34302796}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:34302796}.
CC -!- MASS SPECTROMETRY: Mass=3259.5; Method=MALDI; Note=Monoisotopic mass.;
CC Evidence={ECO:0000269|PubMed:34302796};
CC -!- TOXIC DOSE: PD(50) is 26.4 nmol/g 1 hour after injection into
CC L.cuprina. LD(50) is >100 nmol/g 24 hours after injection into
CC L.cuprina. {ECO:0000269|PubMed:34302796}.
CC -!- SIMILARITY: Belongs to the ponericin-G family. {ECO:0000305}.
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DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR InterPro; IPR010002; Poneritoxin.
DR Pfam; PF07442; Ponericin; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Membrane; Secreted;
KW Target cell membrane; Target membrane; Toxin.
FT PEPTIDE 1..31
FT /note="M-poneritoxin-Nc3b"
FT /evidence="ECO:0000269|PubMed:34302796"
FT /id="PRO_0000454519"
FT MUTAGEN 1
FT /note="Missing: No change in anthelmintic, insecticidal,
FT cytotoxic and hemolytic activities. No change in
FT intracellular calcium concentration on neuronal and
FT epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 3
FT /note="T->W: Moderate increase in anthelmintic,
FT insecticidal and cytotoxic activities. Gain of activity in
FT causing an increase in intracellular calcium concentration
FT on neuronal cell line but no change on epithelial cell
FT lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 5
FT /note="E->D: In (swap)Nc3b; No or weak change in
FT anthelmintic, insecticidal, cytotoxic and hemolytic
FT activities. No change in intracellular calcium
FT concentration on neuronal and epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 19
FT /note="L->P: No or weak change in anthelmintic,
FT insecticidal, cytotoxic and hemolytic activities. No change
FT in intracellular calcium concentration on neuronal and
FT epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 20
FT /note="G->E: No or weak change in anthelmintic,
FT insecticidal, cytotoxic and hemolytic activities. No change
FT in intracellular calcium concentration on neuronal and
FT epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 21
FT /note="M->I: In (swap)Nc3b; No or weak change in
FT anthelmintic, insecticidal, cytotoxic and hemolytic
FT activities. No change in intracellular calcium
FT concentration on neuronal and epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 23
FT /note="S->R: Moderate increase in anthelmintic, and
FT insecticidal activities. Gain of activity in causing an
FT increase in intracellular calcium concentration on neuronal
FT cell line but no change on epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
FT MUTAGEN 30
FT /note="L->I: In (swap)Nc3b; No or weak change in
FT anthelmintic, insecticidal, cytotoxic and hemolytic
FT activities. No change in intracellular calcium
FT concentration on neuronal and epithelial cell lines."
FT /evidence="ECO:0000269|PubMed:34302796"
SQ SEQUENCE 31 AA; 3262 MW; 36ED08090A46566A CRC64;
AGTKEWLNKA KDFIKEKGLG MLSAAANAAL N
