ID   GT_BPN15                Reviewed;         250 AA.
AC   O64329;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2015, sequence version 2.
DT   07-APR-2021, entry version 61.
DE   RecName: Full=Tail assembly protein GT {ECO:0000255|HAMAP-Rule:MF_04134, ECO:0000303|PubMed:10860722};
DE   AltName: Full=Gene product 15 {ECO:0000255|HAMAP-Rule:MF_04134};
DE            Short=gp15 {ECO:0000255|HAMAP-Rule:MF_04134};
DE   AltName: Full=Gene product GT {ECO:0000255|HAMAP-Rule:MF_04134};
DE            Short=gpGT {ECO:0000255|HAMAP-Rule:MF_04134};
DE   AltName: Full=Minor tail protein GT {ECO:0000255|HAMAP-Rule:MF_04134};
GN   Name=gene 15 {ECO:0000312|EMBL:AAC19051.1};
OS   Escherichia phage N15 (Bacteriophage N15).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Ravinvirus.
OX   NCBI_TaxID=40631 {ECO:0000312|Proteomes:UP000002132};
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RX   PubMed=10860722; DOI=10.1006/jmbi.2000.3731;
RA   Ravin V., Ravin N., Casjens S., Ford M.E., Hatfull G.F., Hendrix R.W.;
RT   "Genomic sequence and analysis of the atypical temperate bacteriophage
RT   N15.";
RL   J. Mol. Biol. 299:53-73(2000).
CC   -!- FUNCTION: Promotes tail assembly by creating a scaffold for the tail
CC       tube proteins. Tail assembly proteins G and GT probably wrap the linear
CC       tape measure protein to create a tail assembly scaffold. This allows
CC       the polymerization of the tail tube protein, during which G and GT are
CC       released, therefore they are absent in the mature virion. The tail
CC       assembly protein GT is produced by a rare -1 ribosomal frameshift. The
CC       ratio of translated G/GT is about 20, and this ratio is important for
CC       proper tail assembly. {ECO:0000255|HAMAP-Rule:MF_04134}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with tail tube protein. Interacts
CC       (via N-terminus) with the tail assembly protein G and the tape measure
CC       protein. {ECO:0000255|HAMAP-Rule:MF_04134}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04134}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC         Comment=The tail assembly protein GT is produced by a rare -1
CC         ribosomal frameshift. This expression strategy assures a fixed ration
CC         of G/GT. {ECO:0000250|UniProtKB:P03735};
CC       Name=Tail assembly protein GT;
CC         IsoId=O64329-1; Sequence=Displayed;
CC       Name=Tail assembly protein G;
CC         IsoId=O64328-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform Tail assembly protein GT]: Frameshifted product
CC       of the GT gene, representing 3-4% of the translated protein.
CC       {ECO:0000250|UniProtKB:P03735}.
CC   -!- SIMILARITY: Belongs to the lambda-like tail assembly protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04134}.
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DR   EMBL; AF064539; AAC19051.1; -; Genomic_DNA.
DR   PIR; T13101; T13101.
DR   RefSeq; NP_046910.1; NC_001901.1.
DR   GeneID; 1261654; -.
DR   KEGG; vg:1261654; -.
DR   Proteomes; UP000002132; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04134; GT_LAMBD; 2.
DR   InterPro; IPR009350; Phage_tail_T.
DR   InterPro; IPR010027; Tail_assembly_G.
DR   InterPro; IPR043704; Tail_assembly_GT.
DR   Pfam; PF06894; Phage_TAC_2; 1.
DR   Pfam; PF06223; Phage_tail_T; 1.
DR   TIGRFAMs; TIGR01715; phage_lam_T; 1.
DR   TIGRFAMs; TIGR01674; phage_lambda_G; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Late protein; Reference proteome; Ribosomal frameshifting;
KW   Viral release from host cell; Viral tail assembly.
FT   CHAIN           1..250
FT                   /note="Tail assembly protein GT"
FT                   /id="PRO_0000432906"
SQ   SEQUENCE   250 AA;  27393 MW;  2DC3B767A6C55E36 CRC64;
     MFLKSELLEC NGSSVTLFQL SALQRIEHLE YLKQLEAVEV GDFQAAITFT VKSGAYLVAM
     SLWHGHPLKG SQGENAAAEV AKIQDEVMQT WPTELVAEAE YKVKLLSGMI APVIDEPTSS
     GEERNEPAEP VTAGKALASE LKFAMKLARE FGRPDWRAML AGMSSTEYGD WKIFYQDNYF
     HDAQLDAHFS GLLYTISTLF FADPELTPDS FSILSPAPEA IDIDDPDDDT LMAKAAGISG
     GVRYGPDGSR